Characterization of fungal pathogens (Diaporthe angelicae and D. eres) responsible for umbel browning and stem necrosis on carrot in France

Characterization of fungal pathogens (Diaporthe angelicae and D. eres) responsible for umbel browning and stem necrosis on carrot in France

A collection of 102 Diaporthe isolates was compiled from lesions on carrot, parsley and wild Apiaceae species in France from 2010 to 2014. Molecular typing based on ITS rDNA sequences resulted in the identification of 85 D. angelicae and 17 D. eres isolates. Based on sequences of the 3′ part of the...

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Bibliographic Details
Published in:Plant pathology Vol. 66; no. 2; pp. 239 - 253
Main Authors: Bastide, F., Sérandat, I., Gombert, J., Laurent, E., Morel, E., Kolopp, J., Guillermin, P. L., Hamon, B., Simoneau, P., Berruyer, R., Poupard, P.
Format: Journal Article
Language:English
Published: Oxford Wiley Subscription Services, Inc 01-02-2017
Wiley
Subjects:
Apiaceae
Daucus
Daucus carota subsp. sativus
Diaporthe
Fungicides
fungus detection on seed
Genetic diversity
Lesions
Life Sciences
necrotrophic fungi
Pathogens
Phomopsis dauci
Plant species
Plant tissues
seed production
Vegetables
Daucus carota subsp. sativus
necrotrophic fungi
Apiaceae
Phomopsis dauci
fungus detection on seed
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Summary:A collection of 102 Diaporthe isolates was compiled from lesions on carrot, parsley and wild Apiaceae species in France from 2010 to 2014. Molecular typing based on ITS rDNA sequences resulted in the identification of 85 D. angelicae and 17 D. eres isolates. Based on sequences of the 3′ part of the IGS rDNA, intraspecific variability was analysed for 17 D. angelicae and 13 D. eres isolates from diverse plant species, locations in France, and plant tissues. The genetic diversity was greater for D. angelicae isolates than D. eres isolates. In vitro sensitivity of five D. angelicae and four D. eres isolates to each of nine fungicides was similar for isolates of both species, with a marked variation in fungicide sensitivity depending on the active ingredient. To assess the pathogenicity of D. angelicae and D. eres isolates on carrot, one isolate of each species was inoculated onto umbels in a controlled environment. Typical lesions were observed for both isolates. Carrot crop debris collected from a seed production field in France and placed in controlled conditions produced perithecia and ascospores typical of Diaporthe, that were further characterized molecularly as belonging to D. angelicae. Detection of Diaporthe species on seed lots from three carrot production fields in France was investigated. Both species were detected on seeds by conventional PCR assay, with a greater frequency for D. angelicae than D. eres (67% vs 33%, respectively). Overall, the results highlighted that umbel browning in carrot seed crops in France was mainly caused by D. angelicae.
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content type line 23
ISSN:0032-0862
1365-3059
DOI:10.1111/ppa.12570