Aberrant nodulation response of Vigna umbellata to a Bradyrhizobium japonicum NodZ mutant and nodulation signals

The (Brady)rhizobium nodulation gene products synthesize lipo-chitin oligosaccharide (LCO) signal molecules that induce nodule primordia on legume roots. In spot inoculation assays with roots of Vigna umbellata, Bradyrhizobium elkanii LCO and chemically synthesized LCO induced aberrant nodule struct...

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Bibliographic Details
Published in:	Molecular plant-microbe interactions Vol. 12; no. 9; pp. 766 - 773
Main Authors:	Cohn, J, Stokkermans, T, Kolli, V.K, Day, R.B, Dunlap, J, Carlson, R, Hughes, D, Peters, N.K, Stacey, G
Format:	Journal Article
Language:	English
Published:	St Paul, MN APS Press 01-09-1999 The American Phytopathological Society
Subjects:	Agronomy. Soil science and plant productions Bacterial Proteins Bacteriology biochemical pathways Biological and medical sciences Bradyrhizobium - genetics Bradyrhizobium - physiology Bradyrhizobium japonicum Carbohydrate Sequence colonization Economic plant physiology Fabaceae - microbiology Fucosyltransferases - genetics Fundamental and applied biological sciences. Psychology Genetics lipochitooligosaccharides Lipopolysaccharides - biosynthesis Lipopolysaccharides - chemistry Microbiology Molecular Sequence Data mutants Mutation nodulation NodZ gene oligosaccharides plant development Plant Roots - microbiology Plants, Medicinal root nodules roots Symbiosis Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) Vigna umbellata Symbiosis Oligosaccharide Bradyrhizobium japonicum Vegetal microorganism relation Nodulation Chitin Structure function relationship Vigna umbellata Grain legume Nod factor Leguminosae Dicotyledones Angiospermae Bacteria Spermatophyta Rhizobiaceae Mutation
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Summary:	The (Brady)rhizobium nodulation gene products synthesize lipo-chitin oligosaccharide (LCO) signal molecules that induce nodule primordia on legume roots. In spot inoculation assays with roots of Vigna umbellata, Bradyrhizobium elkanii LCO and chemically synthesized LCO induced aberrant nodule structures, similar to the activity of these LCOs on Glycine soja (soybean). LCOs containing a pentameric chitin backbone and a reducing-end 2-O-methyl fucosyl moiety were active on V. umbellata. In contrast, the synthetic LCO-IV(C(16:0)), which has previously been shown to be active on G. soja, was inactive on V. umbellata. A B. japonicum NodZ mutant, which produces LCO without 2-O-methyl fucose at the reducing end, was able to induce nodule structures on both plants. Surprisingly, the individual, purified, LCO molecules produced by this mutant were incapable of inducing nodule formation on V. umbellata roots. However, when applied in combination, the LCOs produced by the NodZ mutant acted cooperatively to produce nodulelike structures on V. umbellata roots.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0894-0282 1943-7706
DOI:	10.1094/mpmi.1999.12.9.766