Outbreak of acute hemorrhagic conjunctivitis in Maharashtra and Gujarat states of India, caused by coxsackie virus A-24 variant

Acute hemorrhagic conjunctivitis is associated with enteroviruses. Among these, Coxsackie A‐24 variant (CA‐24) and Enterovirus‐70 (EV‐70) are known to cause epidemics and pandemics. An outbreak of acute hemorrhagic conjunctivitis occurred in August–September 2003 in Maharashtra and Gujarat states of...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of medical virology Vol. 79; no. 6; pp. 748 - 753
Main Authors:	Gopalkrishna, V., Patil, P.R., Kolhapure, R.M., Bilaiya, H., Fulmali, P.V., Deolankar, R.P.
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-06-2007 Wiley-Liss
Subjects:	acute hemorrhagic conjunctivitis Adolescent Adult Aged Aged, 80 and over Animals Biological and medical sciences Cell Line, Tumor Child Child, Preschool Conjunctivitis, Acute Hemorrhagic - epidemiology Conjunctivitis, Acute Hemorrhagic - virology Coxsackie virus (CA-24) Coxsackievirus Coxsackievirus Infections - epidemiology Coxsackievirus Infections - virology Disease Models, Animal Disease Outbreaks Enterovirus Enterovirus C, Human - classification Enterovirus C, Human - genetics Enterovirus C, Human - isolation & purification Enterovirus C, Human - pathogenicity Enterovirus-70 (EV-70) Eye - virology Female Fundamental and applied biological sciences. Psychology Human viral diseases Humans India - epidemiology Infectious diseases Male Medical sciences Mice Microbiology Middle Aged Miscellaneous Phylogeny Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - genetics Sequence Analysis, DNA Serotyping Viral diseases Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye Virology Gujarat Maharashtra Asia India Enterovirus-70 (EV-70) Coxsackie virus (CA-24) Picornaviridae Acute Enterovirus 70 Coxsackievirus A acute hemorrhagic conjunctivitis Enterovirus Conjunctiva disease Infection Virus Eye disease Hemorrhagic conjunctivitis Viral disease
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Acute hemorrhagic conjunctivitis is associated with enteroviruses. Among these, Coxsackie A‐24 variant (CA‐24) and Enterovirus‐70 (EV‐70) are known to cause epidemics and pandemics. An outbreak of acute hemorrhagic conjunctivitis occurred in August–September 2003 in Maharashtra and Gujarat states of India. The present investigation was carried out to determine the viral etiological agent associated with the epidemic. Virus isolates were obtained from 11 eye swabs of conjunctivitis patients using HeLa/ Hep‐2 cell lines. The isolates were characterized by serological and mouse pathogenecity tests, RT‐PCR using enterovirus common primers (VP4‐VP2), CA‐24 specific primers (3C‐proteinase region), EV‐70 primers (VP‐3) followed by sequencing, and phylogenetic analysis. The virus was characterized as a Coxsackie A‐24 variant (CA‐24v) and none of the isolates were found to be positive for EV‐70. Sequencing of the PCR products derived from all the 11 isolates revealed 98.4% (SE 0.20) nucleotide identity within the Indian strains and 98.6% (0.50) and 94.4% (0.30) nucleotide identity respectively with the West Indies and Asian strains reported worldwide. The findings suggest that the outbreak of acute hemorrhagic conjunctivitis that occurred in Maharashtra and Gujarat states of India during August–September 2003 was caused by the Coxsackie A‐24 variant (CA‐24v). J. Med. Virol. 79: 748–753, 2007. © 2007 Wiley‐Liss, Inc.
Bibliography:	ark:/67375/WNG-M4K33JG4-W istex:AB6EE6A0E70A65105E24D48E574C56D48095A834 ArticleID:JMV20886 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0146-6615 1096-9071
DOI:	10.1002/jmv.20886