Search Results - "Kolb, V A"

Properties of intraribosomal part of nascent polypeptide by Kolb, V. A

“…This review analyzes the concept according to which the pathway of synthesized peptide from the ribosome peptidyl transferase center to the exit domain goes…”
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Ribosome-associated protein that inhibits translation at the aminoacyl-tRNA binding stage by Agafonov, Dmitry E, Kolb, Vyacheslav A, Spirin, Alexander S

“…We have recently isolated and characterized a novel protein associated with Escherichia coli ribosomes and named protein Y (pY). Here we show that the…”
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Modification of the 5' End of mRNA Leader Sequence Alters the Set of Initiation Factors Essential for Initiation of Translation by Sakharov, P A, Sogorin, E A, Agalarov, S Ch, Kolb, V A

“…The abundance of noncanonical mechanisms of eukaryotic initiation of translation indicates their involvement in the regulation of protein synthesis during key…”
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Modification of the 5' End of mRNA Leader Sequence Alters the Set of Initiation Factors Essential for Initiation of Translation by Sakharov, P. A., Sogorin, E. A., Agalarov, S. Ch, Kolb, V. A.

“…The abundance of noncanonical mechanisms of eukaryotic initiation of translation indicates their involvement in the regulation of protein synthesis during key…”
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Cotranslational Protein Folding by Kolb, V A

“…The review analyzes the research concerning the folding of proteins in the course of their synthesis on ribosomes. The experimental data obtained for various…”
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Trigger factor assists the refolding of heterodimeric but not monomeric luciferases by Melkina, O. E., Goryanin, I. I., Manukhov, I. V., Baranova, A. V., Kolb, V. A., Svetlov, M. S., Zavilgelsky, G. B.

“…The refolding of thermally inactivated protein by ATP-independent trigger factor (TF) and ATP-dependent DnaKJE chaperones was comparatively analyzed…”
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A novel stress-response protein that binds at the ribosomal subunit interface and arrests translation by Agafonov, D E, Kolb, V A, Spirin, A S

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Co-translational Folding of an Eukaryotic Multidomain Protein in a Prokaryotic Translation System by Kolb, Vyacheslav A., Makeyev, Eugeny V., Spirin, Alexander S.

“…Continuous monitoring of the enzymatic activity of newly synthesized firefly luciferase in Escherichia colicell-free translation system was performed to record…”
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Folding of the firefly luciferase polypeptide chain with the immobilized C terminus by Svetlov, M. S., Kolb, V. A., Spirin, A. S.

“…Refolding of firefly Photinus pyralis luciferase from a denatured state is a slow process; its rate and productivity depend on molecular chaperones of the…”
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Cotranslational folding of proteins by Kolb, V A, Makeyev, E V, Kommer, A, Spirin, A S

“…Many unfolded polypeptides are capable of refolding into their native structure upon the removal of the denaturant. However, the folding of the mature protein…”
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A Protein Residing at the Subunit Interface of the Bacterial Ribosome by Agafonov, Dmitry E., Kolb, Vyacheslav A., Nazimov, Igor V., Spirin, Alexander S.

“…Surface labeling of Escherichia coli ribosomes with the use of the tritium bombardment technique has revealed a minor unidentified ribosome-bound protein (spot…”
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Folding of firefly luciferase during translation in a cell‐free system by Kolb, V.A., Makeyev, E.V., Spirin, A.S.

“…In vitro synthesis of firefly luciferase and its folding into an enzymatically active conformation were studied in a wheat germ cell‐free translation system. A…”
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Enzymatic activity of the ribosome-bound nascent polypeptide by Makeyev, Eugeny V., Kolb, Vyacheslav A., Spirin, Alexander S.

“…Firefly luciferase was shown to be completely folded and thus enzymatically active immediately upon release from the ribosome [Kolb et al. (1994) EMBO J. 13,…”
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Cotranslational protein folding by Kolb, V A

“…The review analyzes the research concerning the folding of proteins in the course of their synthesis on ribosomes. The experimental data obtained for various…”
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Proteins on Ribosome Surface: Measurements of Protein Exposure by Hot Tritium Bombardment Technique by Agafonov, Dmitry E., Kolb, Vyacheslav A., Spirin, Alexander S.

“…The hot tritium bombardment technique [Goldanskii, V. I., Kashirin, I. A., Shishkov, A. V., Baratova, L. A. & Grebenshchikov, N. I. (1988) J. Mol. Biol. 201,…”
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Cell-free immunology: construction and in vitro expression of a PCR-based library encoding a single-chain antibody repertoire by Makeyev, Eugeny V., Kolb, Vyacheslav A., Spirin, Alexander S.

“…A novel cloning-independent strategy has been developed to generate a combinatorial library of PCR fragments encoding a murine single-chain antibody repertoire…”
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Synthesis and maturation of green fluorescent protein in a cell-free translation system by Kolb, Vyacheslav A., Makeyev, Eugeny V., Ward, William W., Spirin, Alexander S.

“…A cell-free translation system producing mature green fluorescent protein (GFP) can be a useful tool for studying the mechanism and kinetics of GFP chromophore…”
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Clinical and laboratory parameters in assessing the risk of exacerbations in chronic obstructive pulmonary disease by Kadushkin, A G, Shman, Т V, Goncharik, А V, Germenchuk, I А, Коlb, А V, Taganovich, А D

“…To estimate the significance of measuring the concentrations of cytokines and immunoglobulins and the relative counts of lymphocyte subpopulations in…”
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Folding of the firefly luciferase polypeptide chain with immobilized C-terminus by Svetlov, M S, Kolb, V A, Spirin, A S

“…Refolding of Photinus pyralis firefly luciferase from a denatured state is a slow process; its rate and productivity depend on molecular chaperones of the…”
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Effective cotranslational folding of firefly luciferase without chaperones of the Hsp70 family by Svetlov, Maxim S., Kommer, Aigar, Kolb, Vyacheslav A., Spirin, Alexander S.

“…Molecular chaperones of the Hsp70 family (bacterial DnaK, DnaJ, and GrpE) were shown to be strictly required for refolding of firefly luciferase from a…”
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