Phylogenetic relationships of the G gene sequence of bovine ephemeral fever virus isolated in Japan, Taiwan and Australia

The G gene encoding the neutralization antigen of bovine ephemeral fever virus (BEFV) was characterized in order to define the virus's molecular epidemiology in Japan and the genetic relationships among the Japanese, Taiwanese and Australian isolates. The nucleotide and amino acid sequences of...

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Bibliographic Details
Published in:	Veterinary microbiology Vol. 137; no. 3; pp. 217 - 223
Main Authors:	Kato, Tomoko, Aizawa, Maki, Takayoshi, Katsunori, Kokuba, Tamotsu, Yanase, Tohru, Shirafuji, Hiroaki, Tsuda, Tomoyuki, Yamakawa, Makoto
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 12-06-2009 Amsterdam; New York: Elsevier Elsevier
Subjects:	Amino Acid Sequence amino acid sequences Animals antigenic variation Antigenicity Antigens, Viral - genetics Australia - epidemiology Base Sequence Biological and medical sciences bovine ephemeral fever Bovine ephemeral fever virus Cattle cross reaction disease outbreaks Ephemeral Fever - epidemiology Ephemeral Fever - virology Ephemeral Fever Virus, Bovine - genetics epidemiological studies epitopes etiology Fundamental and applied biological sciences. Psychology G gene genes genetic variation genotype glycoproteins Japan - epidemiology Microbiology Miscellaneous Molecular epidemiology Molecular Sequence Data nucleotide sequences pathogen identification Phylogeny protective effect Rabbits RNA, Viral - genetics strains Taiwan - epidemiology Time Factors vaccines viral antigens Virology Australia Japan Taiwan Asia Oceania Bovine ephemeral fever virus Molecular epidemiology Phylogeny Antigenicity G gene Microbiology Nucleotide sequence Ephemerovirus Genotype Virus Mononegavirales Gene Rhabdoviridae Veterinary
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Summary:	The G gene encoding the neutralization antigen of bovine ephemeral fever virus (BEFV) was characterized in order to define the virus's molecular epidemiology in Japan and the genetic relationships among the Japanese, Taiwanese and Australian isolates. The nucleotide and amino acid sequences of the gene were highly conserved among the Japanese strains, regardless of the year of isolation, and were closely related to the Taiwanese strains. By phylogenetic analysis, the Japanese and Taiwanese strains were classified clearly into three chronological clusters: 1966, 1984–1989 and 1996–2004, indicating that the epidemics of bovine ephemeral fever may occur almost simultaneously in both countries by the same genotype. On the other hand, the Australian strains were distantly related to these East Asian strains and placed in the independent fourth cluster of the phylogenetic tree. It is suggested that three amino acid substitutions at residues 224, 271 and 499 in the neutralizing epitopes, of which two generate new glycosylation sequences, are responsible for antigenic variations of bovine ephemeral fever virus. The cross-neutralization test using the bovine ephemeral fever virus isolated in Japan demonstrated that the vaccine developed based on the oldest Japanese strain, YHL, appears to still be effective for controlling bovine ephemeral fever in Japan.
Bibliography:	http://dx.doi.org/10.1016/j.vetmic.2009.01.021 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0378-1135 1873-2542
DOI:	10.1016/j.vetmic.2009.01.021