Cells in human postmortem brain tissue slices remain alive for several weeks in culture

Animal models for human neurological and psychiatric diseases only partially mimic the underlying pathogenic processes. Therefore, we investigated the potential use of cultured postmortem brain tissue from adult neurological patients and controls. The present study shows that human brain tissue slic...

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Published in:	The FASEB journal Vol. 16; no. 1; pp. 54 - 60
Main Authors:	Verwer, Ronald W. H., Hermens, Wim T. J. M. C., Dijkhuizen, Paul A., Brake, Olivier Ter, Baker, Robert E., Salehi, Ahmad, Sluiter, Arja A., Kok, Marloes J. M., Müller, Linda J., Verhaagen, Joost, Swaab, Dick F.
Format:	Journal Article
Language:	English
Published:	United States 01-01-2002
Subjects:	Aged aging Alzheimer's disease beta-Galactosidase - genetics Brain - cytology Cell Count Cell Survival Cells, Cultured Culture Techniques - methods Dependovirus - genetics Electron Transport Complex IV - metabolism Genetic Vectors human brain Humans Kinetics Middle Aged Motor Cortex - cytology Neurodegenerative Diseases - pathology Neuroglia - metabolism Neurons - drug effects Neurons - metabolism Postmortem Changes Pyruvic Acid - pharmacology tissue culture Transduction, Genetic transgene expression
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Summary:	Animal models for human neurological and psychiatric diseases only partially mimic the underlying pathogenic processes. Therefore, we investigated the potential use of cultured postmortem brain tissue from adult neurological patients and controls. The present study shows that human brain tissue slices obtained by autopsy within 8 h after death can be maintained in vitro for extended periods (up to 78 days) and can be manipulated experimentally. We report for the first time that 1) neurons and glia in such cultures could be induced to express the reporter gene LacZ after transduction with adeno‐associated viral vectors and 2) cytochrome oxidase activity could be enhanced by the addition of pyruvate to the medium. These slice cultures offer new opportunities to study the cellular and molecular mechanisms of neurological and psychiatric diseases and new therapeutic strategies.— Verwer, R. W. H., Hermens, W. T. J. M. C. Dijkhuizen, P. A., ter Brake, O., Baker, R. E., Salehi, A., Sluiter, A. A., Kok, M. J. M., Müller, L. J., Verhaagen, J., Swaab, D. F. Cells in human postmortem brain tissue slices remain alive for several weeks in culture. FASEB J. 16, 54–60 (2002)
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0892-6638 1530-6860
DOI:	10.1096/fj.01-0504com