Search Results - "Koji Ashizawa"

The effects of cysteine addition during in vitro maturation on the developmental competence, ROS, GSH and apoptosis level of bovine oocytes exposed to heat stress by Nabenishi, Hisashi, Ohta, Hiroshi, Nishimoto, Toshihumi, Morita, Tetsuo, Ashizawa, Koji, Tsuzuki, Yasuhiro

“…In the present study, we investigated the effects of various concentrations of cysteine (0.0, 0.6, 1.2 and 1.8 mM) added to the maturation medium on nuclear…”
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Effect of the Temperature-Humidity Index on Body Temperature and Conception Rate of Lactating Dairy Cows in Southwestern Japan by NABENISHI, Hisashi, OHTA, Hiroshi, NISHIMOTO, Toshihumi, MORITA, Tetsuo, ASHIZAWA, Koji, TSUZUKI, Yasuhiro

“…In the present study, we investigated the relationship between the temperature-humidity index (THI) and the conception rate of lactating dairy cows in…”
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The role of mitochondrial transition pores on bovine oocyte competence after heat stress, as determined by effects of cyclosporin A by Nabenishi, Hisashi, Takagi, Satoru, Kamata, Hiroshi, Nishimoto, Toshihumi, Morita, Tetsuo, Ashizawa, Koji, Tsuzuki, Yasuhiro

“…This study was undertaken to investigate the effects on the nuclear maturation and subsequent embryonic development of bovine oocytes exposed to heat stress…”
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Development of a repeated-dose liver micronucleus assay using adult rats: An investigation of diethylnitrosamine and 2,4-diaminotoluene by Narumi, Kazunori, Ashizawa, Koji, Takashima, Rie, Takasawa, Hironao, Katayama, Seiichi, Tsuzuki, Yasuhiro, Tatemoto, Hideki, Morita, Takeshi, Hayashi, Makoto, Hamada, Shuichi

“…► We developed a repeated-dose liver micronucleus (MN) assay using normal adult rats. ► Chemical hepatocarcinogens significantly induced micronucleated…”
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Persistence and accumulation of micronucleated hepatocytes in liver of rats after repeated administration of diethylnitrosamine by Narumi, Kazunori, Ashizawa, Koji, Fujiishi, Yohei, Tochinai, Ryota, Okada, Emiko, Tsuzuki, Yasuhiro, Tatemoto, Hideki, Hamada, Shuichi, Kaneko, Kimiyuki, Ohyama, Wakako

“…•Long-term persistence of MNHEPs was able to observe directly using EdU labeling analysis.•Cumulative model of MNHEP frequency over time was theoretically…”
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Role of Acidification Elicited by Sialylation and Sulfation of Zona Glycoproteins During Oocyte Maturation in Porcine Sperm-zona Pellucida Interactions by LAY, Khin Mar, OSHIRO, Ryuko, ARASAKI, Chiemi, ASHIZAWA, Koji, TATEMOTO, Hideki

“…The porcine zona pellucida (ZP) undergoes biochemical changes during the final phase of maturation prior to fertilization. The present study was conducted to…”
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Characteristics of Okinawan Native Agu Pig Spermatozoa After Addition of Low-Density Lipoprotein to Freezing Extender by YAMAUCHI, Shogo, NAKAMURA, Satoshi, LAY, Khin Mar, AZUMA, Toshiyuki, YAKABI, Tatsuro, MUTO, Norio, NAKADA, Tadashi, ASHIZAWA, Koji, TATEMOTO, Hideki

“…Technical refinement of boar sperm cryopreservation is indispensable for effective breeding of the rare Okinawan native pig, the Agu. The objective of the…”
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Intracellular signal transduction pathways in the regulation of fowl sperm motility: Evidence for the involvement of phosphatidylinositol 3-kinase (PI3-K) cascade by Ashizawa, Koji, Omura, Yusuke, Katayama, Seiichi, Tatemoto, Hideki, Narumi, Kazunori, Tsuzuki, Yasuhiro

“…The possible role of PI3‐K in the reversible temperature‐dependent immobilization of fowl sperm motility was investigated by using PI3‐K inhibitor (LY294002)…”
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Antihyaluronidase action of ellagic acid effectively prevents polyspermy as a result of suppression of the acrosome reaction induced by sperm-Zona interaction during in vitro fertilization of porcine oocytes by Tokeshi, I.(Ryukyu Univ., Nishihara, Okinawa (Japan). Faculty of Agriculture), Yoshimoto, T, Muto, N, Nakamura, S, Ashizawa, K, Nakada, T, Tatemoto, H

“…The present study was conducted to examine the effects of three tannin relatives (tannic acid, TA; gallic acid, GA; and ellagic acid, EA) on antihyaluronidase…”
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In vitro formation of holes on the inner perivitelline layer of quail ovum by chicken spermatozoa by WIN, Mar Mar, MIYAGI, Yuki, ASHIZAWA, Koji, TATEMOTO, Hideki, NAKADA, Tadashi

“…ABSTRACT The aim of the present study was to examine whether chicken semen can be substituted for quail semen to conduct in vitro experiments on fertilization…”
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Effects of Diethylstilbestrol Administration on Sperm Penetration into the Inner Perivitelline Layer of Japanese Quail, Coturnix japonica by Mar M. Win, Hideki Tatemoto, Koji Ashizawa, Tadashi Nakada

“…The aim of this study was to examine the effects of diethylstilbestrol (DES) administration on sperm penetration into the inner perivitelline layer (IPL) of…”
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Determination of Acrosomal Proteolytic Activity in Spermatozoa Collected from Quail Treated with Diethylstilbestrol, Using Gelatin-Substrate Slide Technique by Mar M. Win, Hideki Tatemoto, Koji Ashizawa, Yasuhiro Kawamoto, Tadashi Nakada

“…The present experiment was designed to ascertain whether gelatin-substrate slide technique devised for detection of the proteolytic activity of acrosomal…”
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The Addition of Mitogen-Activated Protein Kinase and p34cdc2Kinase Substrate Peptides Inhibits the Flagellar Motility of Demembranated Fowl Spermatozoa by Ashizawa, Koji, Hashimoto, Kei, Higashio, Mari, Tsuzuki, Yasuhiro

“…The motility of demembranated fowl spermatozoa was vigorous at 30°C, but decreased markedly following the addition of mitogen-activated protein (MAP) kinase or…”
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Presence of protein phosphatase type 1 and its involvement in temperature-dependent flagellar movement of fowl spermatozoa by Ashizawa, Koji, Wishart, Graham J., Tomonaga, Hiroshi, Nishinakama, Kohfumi, Tsuzuki, Yasuhiro

“…Even in the presence of ATP, the motility of demembranated fowl spermatozoa was negligible at the avian body temperature of 40°C. Motility could be restored by…”
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Effects of temperature on the immobilization and the initiation of motility of spermatozoa in the male reproductive tract of the domestic fowl, Gallus domesticus by Ashizawa, K, Sano, R

“…1. The motility of undiluted fowl spermatozoa taken from testis, epididymis and ductus deferens was negligible at 40 degrees C, around the normal avian body…”
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Regulation of flagellar motility by temperature-dependent phosphorylation of a 43 kDa axonemal protein in fowl spermatozoa by Ashizawa, K, Katayama, S, Tsuzuki, Y

“…Phosphorylation of fowl sperm proteins was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) after incubating the demembranated…”
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The effects of extracellular ATP and its receptor antagonists on pig oocytes during in vitro maturation by Wakizoe, Erika, Ashizawa, Koji, Sakamoto, Shinsuke H, Hemmi, Koichiro, Kobayashi, Ikuo, Tsuzuki, Yasuhiro

“…We measured the ATP concentrations in the porcine follicular fluid derived from three sizes of follicles (small: 6 mm in diameter). Then, the effects of…”
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Improvement of the post-thaw qualities of Okinawan native Agu pig sperm frozen in an extender supplemented with antiapoptotic PTD-FNK protein by Shimokawa, Kazuki, Oshiro, Ryuko, Yamanaka, Kenichi, Ashizawa, Koji, Ohta, Shigeo, Tatemoto, Hideki

“…The technical establishment of boar sperm cryopreservation is indispensable for effective breeding of the scarce Okinawan native Agu pig. The objective was to…”
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Regulation of fowl sperm motility: Evidence for the indirect, but not direct, involvement of dynein-ATPase activity on the reversible temperature-dependent immobilization by Ashizawa, Koji, Oyama, Nao, Katayama, Seiichi, Narumi, Kazunori, Tatemoto, Hideki, Tsuzuki, Yasuhiro

“…Potential mechanisms of the reversible temperature-dependent immobilization of fowl sperm were investigated. At 30 °C, motility of demembranated fowl sperm was…”
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N-glycosylation of zona glycoproteins during meiotic maturation is involved in sperm-zona pellucida interactions of porcine oocytes by Lay, K.M., Ashizawa, K., Nakada, T., Tatemoto, H.

“…The objective was to determine whether N-glycosylation of zona pellucida (ZP) glycoproteins occurred during meiotic maturation of porcine oocytes, and whether…”
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