Search Results - "Kohn, L.D."

Thyroglobulin gene expression is regulated by insulin and insulin-like growth factor I, as well as thyrotropin, in FRTL-5 thyroid cells by Santisteban, P., Kohn, L.D., Di Lauro, R.

“…In FRTL-5 thyroid cells depleted of thyrotropin, insulin, and serum for 3 days, growth and synthesis of thyroglobulin are inhibited. Readdition of insulin or…”
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Identification of a novel insulin-responsive element in the rat thyrotropin receptor promoter by Shimura, Y, Shimura, H, Ohmori, M, Ikuyama, S, Kohn, L.D.

“…By transfecting TSH receptor (TSHR)-chloramphenicol acetyltransferase (CAT) chimeras into FRTL-5 thyroid cells in the presence or absence of insulin, we…”
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Characterization of monoclonal antibodies derived from lymphocytes from Graves' disease patients in a cytochemical bioassay for thyroid stimulators by Ealey, P A, Kohn, L D, Ekins, R P, Marshall, N J

“…Monoclonal antibodies 208F7 and 307H6, derived from Graves' lymphocytes, were previously shown to stimulate thyroid function. We characterized these antibodies…”
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Binding of thyroglobulin to bovine thyroid membranes. Role of specific amino acids in receptor recognition by Shifrin, S, Kohn, L D

“…Bovine thyroglobulin was treated with increasing ratios of succinic anhydride, trinitrobenzene sulfonic acid, tetranitromethane, and N-acetylimidazole in an…”
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Cloning, Chromosomal Assignment, and Regulation of the Rat Thyrotropin Receptor: Expression of the Gene is Regulated by Thyrotropin, Agents that Increase cAMP Levels, and Thyroid Autoantibodies by Akamizu, Takashi, Ikuyama, Shoichiro, Saji, Motoyasu, Kosugi, Shinji, Kozak, Christine, McBride, O. Wesley, Kohn, Leonard D.

“…A rat thyrotropin (thyroid-stimulating hormone, TSH) receptor cDNA was isolated that encoded a protein of 764 amino acids, Mr86,528. Transfection of the cDNA…”
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Mutation of alanine 623 in the third cytoplasmic loop of the rat thyrotropin (TSH) receptor results in a loss in the phosphoinositide but not cAMP signal induced by TSH and receptor autoantibodies by KOSUGI, S, OKAJIMA, F, BAN, T, HIDAKA, A, SHENKER, A, KOHN, L. D

“…Thyrotropin (TSH) and IgG preparations from patients with Graves' disease increase inositol phosphate as well as cAMP formation in Cos-7 cells transfected with…”
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Characterization of thyroid-stimulating blocking antibodies that appeared during transient hypothyroidism after radioactive iodine therapy by Kung, A W, Lau, K S, Kohn, L D

“…Hypothyroidism after radioactive iodine (RAI) therapy for Graves' disease can be transient or permanent. The cause for early transient hypothyroidism is…”
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Site-directed mutagenesis of a portion of the extracellular domain of the rat thyrotropin receptor important in autoimmune thyroid disease and nonhomologous with gonadotropin receptors. Relationship of functional and immunogenic domains by KOSUGI, S, BAN, T, AKAMIZU, T, KOHN, L. D

“…Residues 287 to 404 of the rat thyrotropin (TSH) receptor exhibit little homology to gonadotropin receptors. A large segment of this region, residues 303-382,…”
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Glycosylation variants of human TSH selectively activate signal transduction pathways by Schaaf, L., Leiprecht, A., Saji, M., Hübner, U., Usadel, K.H., Kohn, L.D.

“…The oligosaccharide chains of pituitary glycoprotein hormones such as human thyroid-stimulating hormone (hTSH) have been shown to be important in biosynthesis,…”
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Transcriptional repression of the MHC class 1 ge4nes by thyroid stimulating hormone (TSH) involves the PKA induced repressor of transcription ICER & multiple promotor response by Kirshner, S.L., Palmer, L.A., Bodor, J., Saji, M., Kohn, L.D., Singer, D.S.

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Thyrotropin, like luteinizing hormone (LH) and chorionic gonadotropin (CG), increases cAMP and inositol phosphate levels in cells with recombinant human LH/CG receptor by Hidaka, A, Minegishi, T, Kohn, L D

“…Glycoprotein hormones and their receptors are each structurally related; thus, ligand-receptor cross reactivity may exist in pathologic situations, i.e., high…”
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The cAMP response element in the rat thyrotropin receptor promoter. Regulation by each decanucleotide of a flanking tandem repeat uses different, additive, and novel mechanisms by SHIMURA, H, IKUYAMA, S, SHIMURA, Y, KOHN, L. D

“…A decanucleotide tandem repeat (TR) sequence, between -162 and -140 base pairs (bp) of the minimal thyrotropin receptor promoter, decreases gene expression by…”
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Control of c-fos and c-myc proto-oncogene induction in rat thyroid cells in culture by Isozaki, O, Kohn, L D

“…Removal of TSH, insulin, and cortisol from the medium, and decreasing the serum content to 0.2%, abolishes both the proliferate and differentiated state of…”
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Peptide sequences from the hypervariable regions of two monoclonal anti-idiotypic antibodies against the thyrotropin (TSH) receptor are similar to TSH and inhibit TSH-increased cAMP production in FRTL-5 thyroid cells by TAUB, R, JUI-CHOU HSU, GARSKY, V. M, HILL, B. L, ERLANGER, B. F, KOHN, L. D

“…Monoclonal antibodies, D2 and 4G11, selected by the autoantiidiotypic approach following injection of thyrotropin (TSH) into mice, mimic TSH in binding to…”
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Calcium transport mechanisms in membrane vesicles from guinea pig brain synaptosomes by Gill, D L, Grollman, E F, Kohn, L D

“…Ca2+ transport mechanisms were investigated using membrane vesicles prepared from guinea pig brain synaptosomes by hypotonic lysis. Two major mechanisms of…”
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Thyroglobulin interactions with thyroid membranes. Relationship between receptor recognition of N-acetylglucosamine residues and the iodine content of thyroglobulin preparations by Consiglio, E, Shifrin, S, Yavin, Z, Ambesi-Impiombato, F S, Rall, J E, Salvatore, G, Kohn, L D

“…Bovine thyroglobulin has been subjected to sequential glycohydrolase treatment in order to define further the components of the carbohydrate chain which are…”
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Tetanus toxin association with developing neuronal cell cultures. Kinetic parameters and evidence for ganglioside-mediated internalization by Yavin, E, Yavin, Z, Habig, W H, Hardegree, M C, Kohn, L D

“…Rat cerebral neurons maintained in monolayer culture accumulate 125I-labeled tetanus toxin. Accumulation is receptor-mediated; i.e. it can be prevented by…”
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Interaction of fragments B and C of tetanus toxin with neural and thyroid membranes and with gangliosides by Morris, N P, Consiglio, E, Kohn, L D, Habig, W H, Hardegree, M C, Helting, T B

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Thyroid membrane ADP ribosyltransferase activity. Stimulation by thyrotropin and activity in functioning and nonfunctioning rat thyroid cells in culture by De Wolf, M J, Vitti, P, Ambesi-Impiombato, F S, Kohn, L D

“…Bovine thyroid membranes possess both ADP ribosyltransferase and NAD glycohydrolase activities with the same Km values for NAD and the same pH optima. In…”
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Tryptophan residues of cholera toxin and its A and B protomers. Intrinsic fluorescence and solute quenching upon interacting with the ganglioside GM1, oligo-GM1, or dansylated oligo-GM1 by De Wolf, M J, Fridkin, M, Kohn, L D

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