Search Results - "Kohman, Richie"

Light sensitization of DNA nanostructures via incorporation of photo-cleavable spacers by Kohman, Richie E, Han, Xue

“…Using light irradiation as a trigger, large-scale structural reconfiguration of DNA nanostructures is demonstrated. We incorporated photo-cleavable spacers at…”
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Light-Triggered Release of Bioactive Molecules from DNA Nanostructures by Kohman, Richie E, Cha, Susie S, Man, Heng-Ye, Han, Xue

“…Recent innovations in DNA nanofabrication allow the creation of intricately shaped nanostructures ideally suited for many biological applications. To advance…”
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Multiplexed in situ protein imaging using DNA-barcoded antibodies with extended hybridization chain reactions by Wang, Yu, Liu, Xiaoyu, Zeng, Yitian, Saka, Sinem K, Xie, Wenxin, Goldaracena, Isabel, Kohman, Richie E, Yin, Peng, Church, George M

“…Abstract Antibodies have long served as vital tools in biological and clinical laboratories for the specific detection of proteins. Conventional methods employ…”
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Photon-directed multiplexed enzymatic DNA synthesis for molecular digital data storage by Lee, Howon, Wiegand, Daniel J., Griswold, Kettner, Punthambaker, Sukanya, Chun, Honggu, Kohman, Richie E., Church, George M.

“…New storage technologies are needed to keep up with the global demands of data generation. DNA is an ideal storage medium due to its stability, information…”
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Biodegradable Polymer Crosslinker: Independent Control of Stiffness, Toughness, and Hydrogel Degradation Rate by Cha, Chaenyung, Kohman, Richie H., Kong, Hyunjoon

“…Hydrogels are being increasingly studied for use in various biomedical applications including drug delivery and tissue engineering. The successful use of a…”
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A comprehensive library of human transcription factors for cell fate engineering by Ng, Alex H. M., Khoshakhlagh, Parastoo, Rojo Arias, Jesus Eduardo, Pasquini, Giovanni, Wang, Kai, Swiersy, Anka, Shipman, Seth L., Appleton, Evan, Kiaee, Kiavash, Kohman, Richie E., Vernet, Andyna, Dysart, Matthew, Leeper, Kathleen, Saylor, Wren, Huang, Jeremy Y., Graveline, Amanda, Taipale, Jussi, Hill, David E., Vidal, Marc, Melero-Martin, Juan M., Busskamp, Volker, Church, George M.

“…Human pluripotent stem cells (hPSCs) offer an unprecedented opportunity to model diverse cell types and tissues. To enable systematic exploration of the…”
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Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression by Pierson Smela, Merrick D, Kramme, Christian C, Fortuna, Patrick R J, Adams, Jessica L, Su, Rui, Dong, Edward, Kobayashi, Mutsumi, Brixi, Garyk, Kavirayuni, Venkata Srikar, Tysinger, Emma, Kohman, Richie E, Shioda, Toshi, Chatterjee, Pranam, Church, George M

“…An in vitro model of human ovarian follicles would greatly benefit the study of female reproduction. Ovarian development requires the combination of germ cells…”
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Recovery of walking after paralysis by regenerating characterized neurons to their natural target region by Squair, Jordan W., Milano, Marco, de Coucy, Alexandra, Gautier, Matthieu, Skinnider, Michael A., James, Nicholas D., Cho, Newton, Lasne, Anna, Kathe, Claudia, Hutson, Thomas H., Ceto, Steven, Baud, Laetitia, Galan, Katia, Aureli, Viviana, Laskaratos, Achilleas, Barraud, Quentin, Deming, Timothy J., Kohman, Richie E., Schneider, Bernard L., He, Zhigang, Bloch, Jocelyne, Sofroniew, Michael V., Courtine, Gregoire, Anderson, Mark A.

“…Axon regeneration can be induced across anatomically complete spinal cord injury (SCI), but robust functional restoration has been elusive. Whether restoring…”
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From Designing the Molecules of Life to Designing Life: Future Applications Derived from Advances in DNA Technologies by Kohman, Richie E., Kunjapur, Aditya M., Hysolli, Eriona, Wang, Yu, Church, George M.

“…Since the elucidation of its structure, DNA has been at the forefront of biological research. In the past half century, an explosion of DNA‐based technology…”
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A SARS‐CoV‐2 Neutralization Assay Using Single Molecule Arrays by Gilboa, Tal, Cohen, Limor, Cheng, Chi‐An, Lazarovits, Roey, Uwamanzu‐Nna, Augusta, Han, Isaac, Griswold, Kettner, Barry, Nick, Thompson, David B., Kohman, Richie E., Woolley, Ann E., Karlson, Elizabeth W., Walt, David R.

“…Coronavirus disease 2019 (COVID‐19) manifests with high clinical variability and warrants sensitive and specific assays to analyze immune responses in infected…”
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Barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel in situ analyses by Liu, Songlei, Punthambaker, Sukanya, Iyer, Eswar P R, Ferrante, Thomas, Goodwin, Daniel, Fürth, Daniel, Pawlowski, Andrew C, Jindal, Kunal, Tam, Jenny M, Mifflin, Lauren, Alon, Shahar, Sinha, Anubhav, Wassie, Asmamaw T, Chen, Fei, Cheng, Anne, Willocq, Valerie, Meyer, Katharina, Ling, King-Hwa, Camplisson, Conor K, Kohman, Richie E, Aach, John, Lee, Je Hyuk, Yankner, Bruce A, Boyden, Edward S, Church, George M

“…Abstract We present barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel insitu analyses (BOLORAMIS), a reverse transcription-free…”
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Permeabilization of the blood-brain barrier via mucosal engrafting: implications for drug delivery to the brain by Bleier, Benjamin S, Kohman, Richie E, Feldman, Rachel E, Ramanlal, Shreshtha, Han, Xue

“…Utilization of neuropharmaceuticals for central nervous system(CNS) disease is highly limited due to the blood-brain barrier(BBB) which restricts molecules…”
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Minibrains: What's in a Name? by Lopes, Melissa, Kohman, Richie, Lunshof, Jeantine, Paulsen, Bruna, Pigoni, Martina, White, Alexandra, Aach, John, Hyun, Insoo

“…The human brain is incredibly complex and relies on inputs and other senses as well as outgoing connections to human tissues. One needs only to look at a baby…”
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How collaboration between bioethicists and neuroscientists can advance research by Hyun, Insoo, Scharf-Deering, J. C., Sullivan, Sarah, Aach, John Dennis, Arlotta, Paola, Baum, Matthew L., Church, George M., Goldenberg, Aaron, Greely, Henry T., Khoshakhlagh, Parastoo, Kohman, Richie E., Lopes, Melissa, Lowenthal, Caroline, Lu, Aric, Ng, Alex H. M., Pasca, Sergiu P., Paulsen, Bruna, Pigoni, Martina, Scott, Christopher T., Silbersweig, David A., Skylar-Scott, Mark A., Truog, Robert D., Lunshof, Jeantine E.

“…By integrating ongoing bioethical collaboration, neuroscientists can create a positive effect on their research and the knowledge it produces. To this end, we…”
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Leukocyte-Mimicking Stem Cell Delivery via in Situ Coating of Cells with a Bioactive Hyperbranched Polyglycerol by Jeong, Jae Hyun, Schmidt, John J, Kohman, Richie E, Zill, Andrew T, DeVolder, Ross J, Smith, Cartney E, Lai, Mei-Hsiu, Shkumatov, Artem, Jensen, Tor W, Schook, Lawrence G, Zimmerman, Steven C, Kong, Hyunjoon

“…Since stem cells emerged as a new generation of medicine, there are increasing efforts to deliver stem cells to a target tissue via intravascular injection…”
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Expanding homogeneous culture of human primordial germ cell-like cells maintaining germline features without serum or feeder layers by Kobayashi, Mutsumi, Kobayashi, Misato, Odajima, Junko, Shioda, Keiko, Hwang, Young Sun, Sasaki, Kotaro, Chatterjee, Pranam, Kramme, Christian, Kohman, Richie E., Church, George M., Loehr, Amanda R., Weiss, Robert S., Jüppner, Harald, Gell, Joanna J., Lau, Ching C., Shioda, Toshi

“…In vitro expansion of human primordial germ cell-like cells (hPGCLCs), a pluripotent stem cell-derived PGC model, has proved challenging due to rapid loss of…”
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Heterotopic Mucosal Grafting Enables the Delivery of Therapeutic Neuropeptides Across the Blood Brain Barrier by Bleier, Benjamin S., Kohman, Richie E., Guerra, Kevin, Nocera, Angela L., Ramanlal, Shreshtha, Kocharyan, Armine H., Curry, William T., Han, Xue

“…Abstract BACKGROUND: The blood-brain barrier represents a fundamental limitation in treating neurological disease because it prevents all neuropeptides from…”
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Clickable polyglycerol hyperbranched polymers and their application to gold nanoparticles and acid-labile nanocarriers by Zill, Andrew, Rutz, Alexandra L, Kohman, Richie E, Alkilany, Alaaldin M, Murphy, Catherine J, Kong, Hyunjoon, Zimmerman, Steven C

“…A one-step, large-scale preparation of alkyne-containing hyper-branched polyglycerols (HPG) is reported. The HPGs undergo click reactions to organic azides…”
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An integrated pipeline for mammalian genetic screening by Kramme, Christian, Plesa, Alexandru M., Wang, Helen H., Wolf, Bennett, Smela, Merrick Pierson, Guo, Xiaoge, Kohman, Richie E., Chatterjee, Pranam, Church, George M.

“…With the recent advancements in genome editing, next-generation sequencing (NGS), and scalable cloning techniques, scientists can now conduct genetic screens…”
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MegaGate: A toxin-less gateway molecular cloning tool by Kramme, Christian, Plesa, Alexandru M., Wang, Helen H., Wolf, Bennett, Smela, Merrick Pierson, Guo, Xiaoge, Kohman, Richie E., Chatterjee, Pranam, Church, George M.

“…Gateway cloning employs the use of the ccdb toxin and has low colony numbers, making it difficult to apply at scale to clone libraries of cDNA vectors. In this…”
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