Development of Erythroid Progenitors under Erythropoietin Stimulation in Xenopus laevis Larval Liver

Development of Erythroid Progenitors under Erythropoietin Stimulation in Xenopus laevis Larval Liver

Erythroid progenitors that respond to erythropoietin (Epo) are present in the liver of adult Xenopus laevis. However, cells responding to Epo in the larval liver and through the metamorphosis period under hepatic remodeling have not been characterized. In this study, tadpoles were staged using the t...

Full description

Saved in:
Bibliographic Details
Published in:Zoological science Vol. 33; no. 6; p. 575
Main Authors: Okui, Takehito, Hosozawa, Sakiko, Kohama, Satoka, Fujiyama, Shingo, Maekawa, Shun, Muto, Hiroshi, Kato, Takashi
Format: Journal Article
Language:English
Published: Japan 01-12-2016
Subjects:
Animals
Erythroid Precursor Cells - metabolism
Erythropoiesis
Erythropoietin - pharmacology
Gene Expression Regulation, Developmental - physiology
Larva
Liver - embryology
Liver - metabolism
Xenopus - embryology
tadpole
hematopoiesis
liver
erythropoiesis
metamorphosis
African clawed frog
erythropoietin
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Erythroid progenitors that respond to erythropoietin (Epo) are present in the liver of adult Xenopus laevis. However, cells responding to Epo in the larval liver and through the metamorphosis period under hepatic remodeling have not been characterized. In this study, tadpoles were staged using the tables of Nieuwkoop and Faber (NF). Liver cells from pre- (NF56) or post- (NF66) metamorphic stage were cultured in the presence of Epo. β2-globin mRNA expression peaked at day 7 after the start of culture. Larval β2-globin was highly expressed in NF56-derived cells, while adult β2-globinwas detected in those of NF66. In both NF56- and NF66-derived cells, mRNA expression of eporand gata2 peaked at day 5 and days 3-4, respectively. In contrast, gata1 expression peaked at day 6 in NF56 cells and at day 5 in NF66 cells. Half maximal proliferation of erythrocytic blast cells derived from the liver at NF66 was observed at day 3, which was earlier than that of NF56. These results indicate that erythroid progenitors that respond to Xenopus laevis Epo are maintained in pre- and post-metamorphic liver, although the tissue architecture changes dramatically during metamorphosis. Additionally, the globin switching occurred, and/or the erythroid progenitors for larval erythrocytes were replaced by those for adult erythrocytes in the metamorphic liver.
ISSN:0289-0003
DOI:10.2108/zs160040