A molecular and serologic survey of Ehrlichia canis, E. chaffeensis, and E. ewingii in dogs and ticks from Oklahoma

Polymerase chain reaction and Southern hybridization were used to survey for the presence of Ehrlichia canis, Ehrlichia chaffeensis, and Ehrlichia ewingii in blood samples of 65 dogs that harbored ticks from northcentral and northeastern Oklahoma. Dog blood samples were also examined for antibodies...

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Bibliographic Details
Published in:	Veterinary parasitology Vol. 79; no. 4; pp. 325 - 339
Main Authors:	Murphy, George L, Ewing, S.A, Whitworth, Lisa C, Fox, J.Carl, Kocan, A.Alan
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 27-11-1998
Subjects:	Amblyomma americanum Animals Base Sequence Blotting, Southern Canine granulocytic ehrlichiosis Canine monocytic ehrlichiosis Dermacentor variabilis DNA - blood DNA, Bacterial - blood Dog Diseases - epidemiology Dog Diseases - microbiology Dogs Ehrlichia - classification Ehrlichia - genetics Ehrlichia - isolation & purification Ehrlichia canis Ehrlichia chaffeensis Ehrlichia chaffeensis - genetics Ehrlichia chaffeensis - isolation & purification Ehrlichia ewingii Ehrlichiosis - epidemiology Ehrlichiosis - veterinary Geography Human monocytic ehrlichiosis Humans Ixodidae Oklahoma - epidemiology Polymerase chain reaction Polymerase Chain Reaction - methods Rhipicephalus sanguineus Seroepidemiologic Studies Serology Southern hybridization Ticks Ticks - microbiology Oklahoma Dermacentor variabilis Ticks Ehrlichia chaffeensis Ehrlichia ewingii Southern hybridization Serology Canine monocytic ehrlichiosis Canine granulocytic ehrlichiosis Polymerase chain reaction Human monocytic ehrlichiosis Dogs Amblyomma americanum Rhipicephalus sanguineus Ehrlichia canis
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Summary:	Polymerase chain reaction and Southern hybridization were used to survey for the presence of Ehrlichia canis, Ehrlichia chaffeensis, and Ehrlichia ewingii in blood samples of 65 dogs that harbored ticks from northcentral and northeastern Oklahoma. Dog blood samples were also examined for antibodies against E. canis and E. chaffeensis, using an immunofluorescent antibody test. Ten of 65 dogs (15.4%) examined were positive for Ehrlichia spp. by PCR. Four (6.2%) were positive for E. ewingii, 2 (3.1%) for E. canis, and 4 (6.2%) for E. chaffeensis. Seven dogs (10.8%) were seropositive for E. canis or E. chaffeensis. Ticks collected from PCR-positive dogs were examined by PCR for the presence of Ehrlichia DNA. Several groups of ticks were PCR-positive for E. ewingii or E. canis. E. canis was detected in Rhipicephalus sanguineus, which is considered the major vector for that organism. E. ewingii was detected in a larger variety of ticks, including the only known vector Amblyomma americanum, as well as in Dermacentor variabilis and R. sanguineus. Results suggest that Ehrlichia spp. which are canine and human pathogens circulate in dogs in Oklahoma and in several tick species that feed on dogs.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0304-4017 1873-2550
DOI:	10.1016/S0304-4017(98)00179-4