A novel metal-tolerant, solvent and surfactant stable protease from a new strain of Bacillus megaterium

This study reported the discovery of a novel protease from a new strain of Bacillus megaterium isolated from marine sediment in the Gulf of Thailand. Maximum protease production was achieved after 15h at 30°C and pH 5.0, 250rpm. The enzyme was 48.92-fold purified to homogeneity using acetone precipi...

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Bibliographic Details
Published in:	Biocatalysis and agricultural biotechnology Vol. 12; pp. 228 - 235
Main Authors:	Uttatree, Sasithorn, Kobtrakool, Khwanlada, Ketsuk, Apassara, Kaenngam, Wanaree, Thakolprajak, Prachawee, Charoenpanich, Jittima
Format:	Journal Article
Language:	English
Published:	Elsevier Ltd 01-10-2017
Subjects:	Bacillus megaterium Metal-tolerant protease Solvent stable protease Surfactant stable protease Metal-tolerant protease Surfactant stable protease Solvent stable protease Bacillus megaterium
Online Access:	Get full text
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Summary:	This study reported the discovery of a novel protease from a new strain of Bacillus megaterium isolated from marine sediment in the Gulf of Thailand. Maximum protease production was achieved after 15h at 30°C and pH 5.0, 250rpm. The enzyme was 48.92-fold purified to homogeneity using acetone precipitation and Hi-Trap phenyl FF column chromatography. It possesses a relative molecular mass of 60kDa on SDS-PAGE. The enzyme was highly active over a broad range of pH (pH 3.0–12.0) and temperature (10–80°C), and displayed the optimal activity at pH 10.0 and 50°C. It was stable between 60 and 80°C for 168h, showing remarkable stability towards a wide range of pHs and hydrophobic solvents. Metal ions and inhibitors as well as detergents and bleaching agents do not significantly affect the enzyme activity. These promising properties make this enzyme a candidate biocatalyst for future use in harsh industrial processes.
ISSN:	1878-8181 1878-8181
DOI:	10.1016/j.bcab.2017.10.012