Search Results - "Koay, Melissa S T"
-
1
Assembling Enzymatic Cascade Pathways inside Virus-Based Nanocages Using Dual-Tasking Nucleic Acid Tags
Published in Journal of the American Chemical Society (01-02-2017)“…The packaging of proteins into discrete compartments is an essential feature for cellular efficiency. Inspired by Nature, we harness virus-like assemblies as…”
Get full text
Journal Article -
2
Structural Characterization of Native and Modified Encapsulins as Nanoplatforms for in Vitro Catalysis and Cellular Uptake
Published in ACS nano (26-12-2017)“…Recent years have witnessed the emergence of bacterial semiorganelle encapsulins as promising platforms for bio-nanotechnology. To advance the development of…”
Get full text
Journal Article -
3
Self-Sorting of Foreign Proteins in a Bacterial Nanocompartment
Published in Journal of the American Chemical Society (12-03-2014)“…Nature uses bottom-up approaches for the controlled assembly of highly ordered hierarchical structures with defined functionality, such as organelles,…”
Get full text
Journal Article -
4
Assembly and Mechanical Properties of the Cargo-Free and Cargo-Loaded Bacterial Nanocompartment Encapsulin
Published in Biomacromolecules (08-08-2016)“…Prokaryotes mostly lack membranous compartments that are typical of eukaryotic cells, but instead, they have various protein-based organelles. These include…”
Get full text
Journal Article -
5
Predicting the Loading of Virus-Like Particles with Fluorescent Proteins
Published in Biomacromolecules (10-02-2014)“…The virus-like particle (VLP) of the Cowpea Chlorotic Mottle Virus (CCMV) has often been used to encapsulate foreign cargo. Here we show two different rational…”
Get full text
Journal Article -
6
Generation-Dependent Templated Self-Assembly of Biohybrid Protein Nanoparticles around Photosensitizer Dendrimers
Published in Nano letters (11-02-2015)“…In this article, we show the great potential of dendrimers for driving the self-assembly of biohybrid protein nanoparticles. Dendrimers are periodically…”
Get full text
Journal Article -
7
Role of electrostatics in the assembly pathway of a single-stranded RNA virus
Published in Journal of virology (01-09-2014)“…We have recently discovered (R. D. Cadena-Nava et al., J. Virol. 86:3318-3326, 2012, doi:10.1128/JVI.06566-11) that the in vitro packaging of RNA by the capsid…”
Get full text
Journal Article -
8
Chemotherapy pro-drug activation by biocatalytic virus-like nanoparticles containing cytochrome P450
Published in Enzyme and microbial technology (10-06-2014)“…•A bacterial CYP has been encapsulated inside virus-like nanoparticles (VLPs).•CYP-VLPs are able to transform chemotherapy pro-drugs tamoxifen and…”
Get full text
Journal Article -
9
Photoprogramming Allostery in Human Serum Albumin
Published in Bioconjugate chemistry (18-07-2018)“…Developing strategies to interfere with allosteric interactions in proteins not only promises to deepen our understanding of vital cellular processes but also…”
Get full text
Journal Article -
10
A Non-hydrolyzable ATP Derivative Generates a Stable Complex in a Light-inducible Two-component System
Published in The Journal of biological chemistry (04-12-2009)“…Isothermal calorimetry (ITC) measurements yielded the binding constants during complex formation of light-inducible histidine kinases (HK) and their cognate…”
Get full text
Journal Article -
11
Labelling Bacterial Nanocages with Photo-switchable Fluorophores
Published in Chemphyschem (17-06-2016)“…The robustness and biocompatibility of bacterial nanocages holds promise for bio‐nanotechnologies. The propensity of these nano‐carriers to penetrate cells has…”
Get full text
Journal Article -
12
Self-assembly and characterization of small and monodisperse dye nanospheres in a protein cage
Published in Chemical science (Cambridge) (01-01-2014)“…Phthalocyanines (Pc) are dyes in widespread use in materials science and nanotechnology, with numerous applications in medicine, photonics, electronics and…”
Get full text
Journal Article -
13
Phototriggered cargo release from virus-like assemblies
Published in Faraday discussions (01-01-2013)“…There has been tremendous progress towards the development of responsive polymers that are programmed to respond to an external stimulus such as light, pH and…”
Get more information
Journal Article -
14
Self-Assembled Cage-Like Protein Structures
Published in Chemphyschem (07-04-2015)“…Proteins and protein‐based assemblies represent the most structurally and functionally diverse molecules found in nature. Protein cages, viruses and bacterial…”
Get full text
Journal Article -
15
Self-assembly triggered by self-assembly: Optically active, paramagnetic micelles encapsulated in protein cage nanoparticles
Published in Journal of inorganic biochemistry (01-07-2014)“…In this contribution, optically active and paramagnetic micelles of the ligand 1,4,7,10-tetraaza-1-(1-carboxymethylundecane)-4,7,10-triacetic acid…”
Get full text
Journal Article -
16
Inside Back Cover: Labelling Bacterial Nanocages with Photo-switchable Fluorophores (ChemPhysChem 12/2016)
Published in Chemphyschem (17-06-2016)“…The Inside Back Cover picture shows protein‐based nanocages of Brevibacterium linens labeled with photo‐switchable fluorophores to facilitate their imaging by…”
Get full text
Journal Article -
17
Recombinant expression and purification of "virus-like" bacterial encapsulin protein cages
Published in Methods in molecular biology (Clifton, N.J.) (01-01-2015)“…Ultracentrifugation, particularly the use of sucrose or cesium chloride density gradients, is a highly reliable and efficient technique for the purification of…”
Get more information
Journal Article