Search Results - "Könning, Doreen"

Beyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface display by Könning, Doreen, Kolmar, Harald

“…Pioneered exactly 20 years ago, yeast surface display (YSD) continues to take a major role in protein engineering among the high-throughput display…”
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Site-Specific Conjugation of Native Antibodies Using Engineered Microbial Transglutaminases by Dickgiesser, Stephan, Rieker, Marcel, Mueller-Pompalla, Dirk, Schröter, Christian, Tonillo, Jason, Warszawski, Shira, Raab-Westphal, Sabine, Kühn, Stefanie, Knehans, Tim, Könning, Doreen, Dotterweich, Julia, Betz, Ulrich A. K, Anderl, Jan, Hecht, Stefan, Rasche, Nicolas

“…Site-specific bioconjugation technologies are frequently employed to generate homogeneous antibody–drug conjugates (ADCs) and are generally considered superior…”
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Generation and Characterization of Iduronidase-Cleavable ADCs by Jäger, Sebastian, Könning, Doreen, Rasche, Nicolas, Hart, Felix, Sensbach, Janike, Krug, Carina, Raab-Westphal, Sabine, Richter, Konstantin, Unverzagt, Carlo, Hecht, Stefan, Anderl, Jan, Schröter, Christian

“…A crucial design feature for the therapeutic success of antibody-drug conjugates (ADCs) is the linker that connects the antibody with the drug. Linkers must be…”
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Effect of Conjugation Site and Technique on the Stability and Pharmacokinetics of Antibody-Drug Conjugates by Kaempffe, Anna, Dickgiesser, Stephan, Rasche, Nicolas, Paoletti, Andrea, Bertotti, Elisa, De Salve, Ilse, Sirtori, Federico Riccardi, Kellner, Roland, Könning, Doreen, Hecht, Stefan, Anderl, Jan, Kolmar, Harald, Schröter, Christian

“…Appropriate selection of conjugation sites and conjugation technologies is now widely accepted as crucial for the success of antibody-drug conjugates (ADCs)…”
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Structural insights and biomedical potential of IgNAR scaffolds from sharks by Zielonka, Stefan, Empting, Martin, Grzeschik, Julius, Könning, Doreen, Barelle, Caroline J, Kolmar, Harald

“…In addition to antibodies with the classical composition of heavy and light chains, the adaptive immune repertoire of sharks also includes a heavy-chain only…”
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A simplified procedure for antibody engineering by yeast surface display: Coupling display levels and target binding by ribosomal skipping by Grzeschik, Julius, Hinz, Steffen C., Könning, Doreen, Pirzer, Thomas, Becker, Stefan, Zielonka, Stefan, Kolmar, Harald

“…Yeast surface display is a valuable, widely used method for protein engineering. However, current yeast display applications rely on the staining of epitope…”
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Semi-synthetic vNAR libraries screened against therapeutic antibodies primarily deliver anti-idiotypic binders by Könning, Doreen, Rhiel, Laura, Empting, Martin, Grzeschik, Julius, Sellmann, Carolin, Schröter, Christian, Zielonka, Stefan, Dickgießer, Stephan, Pirzer, Thomas, Yanakieva, Desislava, Becker, Stefan, Kolmar, Harald

“…Anti-idiotypic binders which specifically recognize the variable region of monoclonal antibodies have proven to be robust tools for pharmacokinetic studies of…”
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Isolation of a pH-Sensitive IgNAR Variable Domain from a Yeast-Displayed, Histidine-Doped Master Library by Könning, Doreen, Zielonka, Stefan, Sellmann, Carolin, Schröter, Christian, Grzeschik, Julius, Becker, Stefan, Kolmar, Harald

“…In recent years, engineering of pH-sensitivity into antibodies as well as antibody-derived fragments has become more and more attractive for biomedical and…”
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Shark attack: Haiantikörper für Biomedizin und Biotechnologie by Könning, Doreen, Grzeschik, Julius, Hinz, Steffen C., Krah, Simon, Empting, Martin, Kolmar, Harald, Zielonka, Stefan

“…The adaptive immune system of sharks comprises a very interesting antibody isotype named immunoglobulin new antigen receptor (IgNAR). In contrast to classical…”
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Camelid and shark single domain antibodies: structural features and therapeutic potential by Könning, Doreen, Zielonka, Stefan, Grzeschik, Julius, Empting, Martin, Valldorf, Bernhard, Krah, Simon, Schröter, Christian, Sellmann, Carolin, Hock, Björn, Kolmar, Harald

“…•Camelids and sharks comprise heavy chain-only antibodies devoid of light chains.•Their variable domains are able to address cryptic and recessed…”
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Isolation of Tailor-Made Antibody Fragments from Yeast-Displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting by Kaempffe, Anna, Jäger, Sebastian, Könning, Doreen, Kolmar, Harald, Schröter, Christian

“…In the past decades, monoclonal antibodies have made an unprecedented transformation from research tools to a rapidly growing class of therapeutics…”
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Selection and Characterization of Anti-idiotypic Shark Antibody Domains by Könning, Doreen, Zielonka, Stefan, Kaempffe, Anna, Jäger, Sebastian, Kolmar, Harald, Schröter, Christian

“…The antibody repertoire of cartilaginous fish comprises an additional heavy-chain-only antibody isotype that is referred to as IgNAR (immunoglobulin novel…”
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Isolation of pH-Sensitive Antibody Fragments by Fluorescence-Activated Cell Sorting and Yeast Surface Display by Schröter, Christian, Krah, Simon, Beck, Jan, Könning, Doreen, Grzeschik, Julius, Valldorf, Bernhard, Zielonka, Stefan, Kolmar, Harald

“…Fluorescence-activated cell sorting (FACS) in combination with yeast surface display (YSD) has proven to be a valuable tool for the engineering of antibodies…”
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Generation of Semi-Synthetic Shark IgNAR Single-Domain Antibody Libraries by Grzeschik, Julius, Könning, Doreen, Hinz, Steffen C, Krah, Simon, Schröter, Christian, Empting, Martin, Kolmar, Harald, Zielonka, Stefan

“…Besides classical antibodies with the composition of heavy and light chains, sharks produce a unique heavy chain only isotype, termed Immunoglobulin New…”
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Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires by Jäger, Sebastian, Krah, Simon, Könning, Doreen, Rosskopf, Janis, Dickgiesser, Stephan, Rasche, Nicolas, Kolmar, Harald, Hecht, Stefan, Schröter, Christian

“…Anti-hapten antibodies are widely used as specific immunochemical detection tools in a variety of clinical and environmental analyses. The sensitivity,…”
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Shark Strikes Twice: Hypervariable Loop 2 of Shark IgNAR Antibody Variable Domains and Its Potential to Function as an Autonomous Paratope by Zielonka, Stefan, Empting, Martin, Könning, Doreen, Grzeschik, Julius, Krah, Simon, Becker, Stefan, Dickgießer, Stephan, Kolmar, Harald

“…In this present study, we engineered hypervariable loop 2 (HV2) of the IgNAR variable domain in a way that it solely facilitates antigen binding, potentially…”
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From pico to nano: biofunctionalization of cube-octameric silsesquioxanes by peptides and miniproteins by Fabritz, Sebastian, Hörner, Sebastian, Könning, Doreen, Empting, Martin, Reinwarth, Michael, Dietz, Christian, Glotzbach, Bernhard, Frauendorf, Holm, Kolmar, Harald, Avrutina, Olga

“…Polyhedral silsesquioxanes are considered valuable conjugation scaffolds. Nevertheless, only a few examples of silsesquioxane-assembled peptide oligomers have…”
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