cDNA and structural organization of the gene Pole1 for the mouse DNA polymerase ϵ catalytic subunit

The cDNA and the gene for the mouse DNA polymerase ϵ catalytic subunit were cloned. The deduced protein sequence shows remarkable evolutionary conservation in DNA polymerase ϵ family. However, several conserved elements involved in template–primer binding differ from those of other class B polymeras...

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Bibliographic Details
Published in:	Biochimica et biophysica acta Vol. 1445; no. 3; pp. 363 - 371
Main Authors:	Huang, Deqi, Knuuti, Riikka, Palosaari, Heidi, Pospiech, Helmut, Syväoja, Juhani E.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 09-06-1999
Subjects:	Amino Acid Sequence Animals Binding Sites Catalysis cDNA cloning Chromosomal mapping DNA polymerase epsilon DNA Polymerase II - chemistry DNA Polymerase II - genetics DNA, Complementary - chemistry Exons Gene structure Introns Mice Molecular Sequence Data Non-conforming splice site Poly-ADP-Ribose Binding Proteins Non-conforming splice site cDNA cloning Chromosomal mapping DNA polymerase epsilon Gene structure
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Summary:	The cDNA and the gene for the mouse DNA polymerase ϵ catalytic subunit were cloned. The deduced protein sequence shows remarkable evolutionary conservation in DNA polymerase ϵ family. However, several conserved elements involved in template–primer binding differ from those of other class B polymerases. This is likely to reflect a distinctive function of the enzyme. The gene that was assigned to chromosome 5 region E3–E5, consists of 49 exons and has a non-conforming splice site in the junction of exon and intron 13. A CpG island covers the promoter region which contains several putative consensus elements critical for S phase upregulated and serum responsive promoters.
ISSN:	0167-4781 0006-3002 1879-2634
DOI:	10.1016/S0167-4781(99)00058-5