Search Results - "Knotts, Thomas A"

A coarse grain model for DNA by Knotts, 4th, Thomas A, Rathore, Nitin, Schwartz, David C, de Pablo, Juan J

“…Understanding the behavior of DNA at the molecular level is of considerable fundamental and engineering importance. While adequate representations of DNA exist…”
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A coarse grain model for protein-surface interactions by Wei, Shuai, Knotts, 4th, Thomas A

“…The interaction of proteins with surfaces is important in numerous applications in many fields-such as biotechnology, proteomics, sensors, and medicine--but…”
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Probing the effects of surface hydrophobicity and tether orientation on antibody-antigen binding by Bush, Derek B, Knotts, 4th, Thomas A

“…Antibody microarrays have the potential to revolutionize molecular detection for many applications, but their current use is limited by poor reliability, and…”
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The effects of antigen size, binding site valency, and flexibility on fab-antigen binding near solid surfaces by Bush, Derek B, Knotts, 4th, Thomas A

“…Next generation antibody microarray devices have the potential to outperform current molecular detection methods and realize new applications in medicine,…”
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Assessing site-specific PEGylation of TEM-1 β-lactamase with cell-free protein synthesis and coarse-grained simulation by Zhao, Emily Long, Soltani, Mehran, Smith, Addison K., Hunt, J. Porter, Knotts, Thomas A., Bundy, Bradley C.

“…PEGylation is a broadly used strategy to enhance the pharmacokinetic properties of therapeutic proteins. It is well established that the location and extent of…”
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Communication: Antibody stability and behavior on surfaces by Bush, Derek B, Knotts, 4th, Thomas A

“…Antibody microarrays have the potential to revolutionize molecular detection in scientific, medical, and other biosensor applications, but their current use is…”
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The Effects of p‑Azidophenylalanine Incorporation on Protein Structure and Stability by Wilkerson, Joshua W, Smith, Addison K, Wilding, Kristen M, Bundy, Bradley C, Knotts, Thomas A

“…Functionalization is often needed to harness the power of proteins for beneficial use but can cause losses to stability and/or activity. State of the art…”
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Thermodynamics of DNA hybridization on surfaces by Schmitt, Terry J, Knotts, 4th, Thomas A

“…Hybridization of single-stranded DNA (ssDNA) targets to surface-tethered ssDNA probes was simulated using an advanced coarse-grain model to identify key…”
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Predicting stability of alpha-helical, orthogonal-bundle proteins on surfaces by Wei, Shuai, Knotts, 4th, Thomas A

“…The interaction of proteins with surfaces is a key phenomenon in many applications, but current understanding of the biophysics involved is lacking. At…”
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Exploring the mechanisms of DNA hybridization on a surface by Schmitt, Terry J, Rogers, J Brandon, Knotts, 4th, Thomas A

“…DNA microarrays are a potentially disruptive technology in the medical field, but their use in such settings is limited by poor reliability. Microarrays work…”
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Assessing the predictive capabilities of design heuristics and coarse‐grain simulation toward understanding and optimizing site‐specific covalent immobilization of β‐lactamase by Soltani, Mehran, Hunt, John Porter, Smith, Addison K., Zhao, Emily Long, Knotts, Thomas A., Bundy, Bradley C.

“…For industrial applications, covalent immobilization of enzymes provides minimum leakage, recoverability, reusability, and high stability. Yet, the suitability…”
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An Entropic Perspective of Protein Stability on Surfaces by Knotts, Thomas A., Rathore, Nitin, de Pablo, Juan J.

“…The interaction of proteins with surfaces regulates numerous processes in nature, science, and technology. In many applications, it is desirable to place…”
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The effects of multiple probes on the hybridization of target DNA on surfaces by Welling, Ryan C, Knotts, 4th, Thomas A

“…DNA microarrays have disruptive potential in many fields including genetics and medicine, but the technology has yet to find widespread clinical use due to…”
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Communication: Using multiple tethers to stabilize proteins on surfaces by Loong, Brandon K, Knotts, 4th, Thomas A

“…Protein surface interactions are important in many applications in biotechnology including protein arrays, but these technologies have not lived up to their…”
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The Locational Impact of Site-Specific PEGylation: Streamlined Screening with Cell-Free Protein Expression and Coarse-Grain Simulation by Wilding, Kristen M, Smith, Addison K, Wilkerson, Joshua W, Bush, Derek B, Knotts, Thomas A, Bundy, Bradley C

“…Although polyethylene glycol (PEG) is commonly used to improve protein stability and therapeutic efficacy, the optimal location for attaching PEG onto proteins…”
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Effects of tethering a multistate folding protein to a surface by Wei, Shuai, Knotts, 4th, Thomas A

“…Protein/surface interactions are important in a variety of fields and devices, yet fundamental understanding of the relevant phenomena remains fragmented due…”
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Multiple molecule effects on the cooperativity of protein folding transitions in simulations by Lewis, Jacob I, Moss, Devin J, Knotts, 4th, Thomas A

“…Though molecular simulation of proteins has made notable contributions to the study of protein folding and kinetics, disagreement between simulation and…”
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Confinement Effects on the Thermodynamics of Protein Folding: Monte Carlo Simulations by Rathore, Nitin, Knotts, Thomas A., de Pablo, Juan J.

“…The effects of chaperonin-like cage-induced confinement on protein stability have been studied for molecules of varying sizes and topologies. Minimalist models…”
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Effects of Chain Grafting Positions and Surface Coverage on Conformations of Model Reversed-Phase Liquid Chromatography Stationary Phases by Mansfield, Eric R, Mansfield, Danielle S, Patterson, James E, Knotts, Thomas A

“…Chemical separations in reversed-phase liquid chromatography (RPLC) are made possible by the detailed molecular-level interactions that take place between…”
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Thermal and mechanical multistate folding of ribonuclease H by Schmitt, Terry J, Clark, Jonathan E, Knotts, 4th, Thomas A

“…Two different classes of experimental techniques exist by which protein folding mechanisms are ascertained. The first class, of which circular dichroism is an…”
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