Genetic effects of space hadrons on bacteriophage under Alpine conditions

A dried film culture of bacteriophage T4Br + was kept in a lead bioblock for 366 days under Alpine conditions at an altitude of 6100 m above sea level to study the genetic effect of space hadrons. In the gelatin-like film under study we discovered some film plots with markedly reduced bacteriophage...

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Bibliographic Details
Published in:	Life sciences and space research Vol. 18; p. 159
Main Authors:	Yurov, S S, Belkin, V S, Leont'eva, G A, Knjaseva, I N, Mozgovoy, E G, Kuzin, A M, Akoev, I G
Format:	Journal Article
Language:	English
Published:	Netherlands 1980
Subjects:	Altitude Bacteriophage T4 - genetics Bacteriophage T4 - growth & development Bacteriophage T4 - radiation effects Cold Temperature Cosmic Radiation DNA Damage DNA, Viral Dose-Response Relationship, Radiation Mutation Relative Biological Effectiveness
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Summary:	A dried film culture of bacteriophage T4Br + was kept in a lead bioblock for 366 days under Alpine conditions at an altitude of 6100 m above sea level to study the genetic effect of space hadrons. In the gelatin-like film under study we discovered some film plots with markedly reduced bacteriophage survival. In such plots, the mutation frequency exceeded the spontaneous background mutation rate 60-100 times. The spectrum of r mutations as classified into standard groups rI, rII and rIII differed from that found for other model radiation systems such as gamma-ray radiation in buffer or nutrient broth, and hadron and HZE particle radiation under space flight conditions. Reversion analysis of 159 rII mutants showed that 54.4% had small and elongated deletions, 23.16% had point mutations, and 22.5% of all the mutants had both small deletion and point mutations.
ISSN:	0075-9422
DOI:	10.1016/B978-0-08-024436-5.50021-4