RNA synthesis in embryogenesis of Drosophila virilis

The synthesis of different RNA fractions labelled by 3H-uridine has been studied at different developmental stages in Drosophila virilis by means of thermal phenol extraction and chromatography on oligo-alphaT-cellulose. There are two periods of intensive synthesis of poly-A containing (messenger) R...

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Bibliographic Details
Published in:	Ontogenez Vol. 7; no. 3; p. 289
Main Authors:	Karasik, G I, Maksimovskiĭ, L F, Knizhnikova, T P, Korochkin, L I
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 1976
Subjects:	Animals Cell Differentiation Chromatography - methods Drosophila - embryology Embryonic Induction Female Phenols RNA - biosynthesis RNA, Messenger - biosynthesis Temperature Tritium Uridine Zygote - metabolism
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Summary:	The synthesis of different RNA fractions labelled by 3H-uridine has been studied at different developmental stages in Drosophila virilis by means of thermal phenol extraction and chromatography on oligo-alphaT-cellulose. There are two periods of intensive synthesis of poly-A containing (messenger) RNA in the course of embryogenesis. The former (6--12 hrs) is the period of gastrulation and stomodeum development and the latter (15--21 hrs) is the period of intensive differentiation of larval organs. The synthesis of poly-A free RNAs predominates at the stages of blastoderm (2--5 hrs) and mesoderm segmentation and embryo shortening (12--15 hrs).
ISSN:	0475-1450