Search Results - "Knecht, David"

Self-Generated Chemoattractant Gradients: Attractant Depletion Extends the Range and Robustness of Chemotaxis by Tweedy, Luke, Knecht, David A, Mackay, Gillian M, Insall, Robert H

“…Chemotaxis is fundamentally important, but the sources of gradients in vivo are rarely well understood. Here, we analyse self-generated chemotaxis, in which…”
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Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum by Paschke, Peggy, Knecht, David A, Silale, Augustinas, Traynor, David, Williams, Thomas D, Thomason, Peter A, Insall, Robert H, Chubb, Jonathan R, Kay, Robert R, Veltman, Douwe M

“…Dictyostelium has a mature technology for molecular-genetic manipulation based around transfection using several different selectable markers, marker…”
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Chemotaxis: Under Agarose Assay by Brazill, Derrick, Knecht, David A

“…The unicellular eukaryotic amoeba, Dictyostelium discoideum, represents a superb model for examining the molecular mechanism of chemotaxis. Under vegetative…”
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Melanoma cells break down LPA to establish local gradients that drive chemotactic dispersal by Muinonen-Martin, Andrew J, Susanto, Olivia, Zhang, Qifeng, Smethurst, Elizabeth, Faller, William J, Veltman, Douwe M, Kalna, Gabriela, Lindsay, Colin, Bennett, Dorothy C, Sansom, Owen J, Herd, Robert, Jones, Robert, Machesky, Laura M, Wakelam, Michael J O, Knecht, David A, Insall, Robert H

“…The high mortality of melanoma is caused by rapid spread of cancer cells, which occurs unusually early in tumour evolution. Unlike most solid tumours,…”
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The phagocytosis and toxicity of amorphous silica by Costantini, Lindsey M, Gilberti, Renée M, Knecht, David A

“…Inhalation of crystalline silica is known to cause an inflammatory reaction and chronic exposure leads to lung fibrosis and can progress into the disease,…”
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PIP₃-dependent macropinocytosis is incompatible with chemotaxis by Veltman, Douwe M, Lemieux, Michael G, Knecht, David A, Insall, Robert H

“…In eukaryotic chemotaxis, the mechanisms connecting external signals to the motile apparatus remain unclear. The role of the lipid phosphatidylinositol…”
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Silica phagocytosis causes apoptosis and necrosis by different temporal and molecular pathways in alveolar macrophages by Joshi, Gaurav N., Knecht, David A.

“…Chronic inhalation of crystalline silica is an occupational hazard that results in silicosis due to the toxicity of silica particles to lung cells. Alveolar…”
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Silica particles cause NADPH oxidase-independent ROS generation and transient phagolysosomal leakage by Joshi, Gaurav N, Goetjen, Alexandra M, Knecht, David A

“…Chronic inhalation of silica particles causes lung fibrosis and silicosis. Silica taken up by alveolar macrophages causes phagolysosomal membrane damage and…”
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Macrophages phagocytose nonopsonized silica particles using a unique microtubule-dependent pathway by Gilberti, Renée M, Knecht, David A

“…Silica inhalation leads to the development of the chronic lung disease silicosis. Macrophages are killed by uptake of nonopsonized silica particles, and this…”
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Visualization of the actin cytoskeleton: Different F-actin-binding probes tell different stories by Lemieux, Michael G., Janzen, Dani, Hwang, Rander, Roldan, Jeannette, Jarchum, Irene, Knecht, David A.

“…The actin cytoskeleton is necessary for cell viability and plays crucial roles in cell motility, endocytosis, growth, and cytokinesis. Hence visualization of…”
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Micro-Acoustic-Trap (µAT) for microparticle assembly in 3D by Vyas, Varun, Lemieux, Michael, Knecht, David A., Kolosov, Oleg V., Huey, Bryan D.

“…[Display omitted] A true non-intrusive 3D Micro-Acoustic Trap for a controlled monolayer assembly of microparticles in aqueous environment. Modulation of…”
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Bacterial metallothionein, PmtA, a novel stress protein found on the bacterial surface of Pseudomonas aeruginosa and involved in management of oxidative stress and phagocytosis by Maltz-Matyschsyk, Michele, Melchiorre, Clare K, Knecht, David A, Lynes, Michael A

“…Metallothioneins (MTs) are small cysteine-rich proteins that play important roles in homeostasis and protection against heavy metal toxicity and oxidative…”
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Teaching a biology laboratory course using Dictyostelium by Knecht, David A, Cooper, Kate M, Moore, Jonathan E

“…The Dictyostelium discoideum model system is a powerful tool for undergraduate cell biology teaching laboratories. The cells are biologically safe, grow at…”
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Cucurbitacin I inhibits cell motility by indirectly interfering with actin dynamics by Knecht, David A, LaFleur, Rebecca A, Kahsai, Alem W, Argueta, Christian E, Beshir, Anwar B, Fenteany, Gabriel

“…Cucurbitacins are plant natural products that inhibit activation of the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3)…”
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Traction force microscopy in Dictyostelium reveals distinct roles for myosin II motor and actin-crosslinking activity in polarized cell movement by Lombardi, Maria L, Knecht, David A, Dembo, Micah, Lee, Juliet

“…Continuous cell movement requires the coordination of protrusive forces at the leading edge with contractile forces at the rear of the cell. Myosin II is…”
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Phosphatidylinositol-4,5-bisphosphate hydrolysis directs actin remodeling during phagocytosis by Scott, Cameron C, Dobson, Wendy, Botelho, Roberto J, Coady-Osberg, Natasha, Chavrier, Philippe, Knecht, David A, Heath, Colin, Stahl, Philip, Grinstein, Sergio

“…The Rho GTPases play a critical role in initiating actin polymerization during phagocytosis. In contrast, the factors directing the disassembly of F-actin…”
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Single Cell Analysis of Phagocytosis, Phagosome Maturation, Phagolysosomal Leakage, and Cell Death Following Exposure of Macrophages to Silica Particles by Joshi, Gaurav N, Gilberti, Renée M, Knecht, David A

“…Chronic inhalation of silica in various occupational settings results in the development of silicosis, a disease characterized by lung fibrosis. Uptake of…”
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The Phagocytosis of Crystalline Silica Particles by Macrophages by Gilberti, Renee M, Joshi, Gaurav N, Knecht, David A

“…Silicosis is a chronic lung disease induced by the inhalation of crystalline silica. Exposure of cultured macrophages to crystalline silica leads to cell…”
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Cross-linking of actin filaments by myosin II is a major contributor to cortical integrity and cell motility in restrictive environments by Laevsky, Gary, Knecht, David A

“…Cells are frequently required to move in a local environment that physically restricts locomotion, such as during extravasation or metastatic invasion. In…”
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Contribution of Filopodia to Cell Migration: A Mechanical Link between Protrusion and Contraction by Xue, Fei, Janzen, Deanna M., Knecht, David A.

“…Numerous F-actin containing structures are involved in regulating protrusion of membrane at the leading edge of motile cells. We have investigated the…”
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