Search Results - "Knauf, P. A."

Development of an spFRET method to measure structure changes in ion exchange proteins by Lesoine, J. F., Holmberg, B., Maloney, P., Wang, X., Novotny, L., Knauf, P. A.

“…Aim:  Understanding the mechanism of tightly coupled ion exchange proteins, important effectors of cell volume regulation and other physiologically important…”
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Acute cell volume changes in anisotonic media affect F-actin content of HL-60 cells by Hallows, K R, Packman, C H, Knauf, P A

“…To investigate the possible role of the cytoskeleton in volume regulatory responses of human promyelocytic leukemic (HL-60) cells, we monitored and modulated…”
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Swelling-activated amino acid efflux in the human neuroblastoma cell line CHP-100 by Basavappa, S, Huang, C C, Mangel, A W, Lebedev, D V, Knauf, P A, Ellory, J C

“…1. The effects of hypoosmotic stress on cell volume and amino acid efflux were evaluated in the human neuroblastoma cell line CHP-100 with the Coulter Counter…”
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Volume-activated chloride channels in HL-60 cells: potent inhibition by an oxonol dye by Arreola, J, Hallows, K R, Knauf, P A

“…When swollen in hypotonic media, HL-60 cells exhibit a regulatory volume decrease (RVD) response as a result of net losses of K+ and Cl-. This is primarily…”
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An oxonol dye is the most potent known inhibitor of band 3-mediated anion exchange by Knauf, P A, Law, F Y, Hahn, K

“…When cells are acutely exposed to the oxonol dye, bis(1,3-dibutylbarbituric acid)pentamethine oxonol (diBA), at 0 degrees C, the concentration that gives half…”
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Regulatory volume decrease in HL-60 cells: importance of rapid changes in permeability of Cl- and organic solutes by Hallows, K R, Knauf, P A

“…Results obtained through the use of inhibitors and isotope flux and equilibration techniques indicate that the regulatory volume decrease (RVD) response of…”
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Lys-430, site of irreversible inhibition of band 3 Cl- flux by eosin-5-maleimide, is not at the transport site by Liu, S Q, Knauf, P A

“…Although eosin-5-maleimide (EM) covalently labels band 3 and has been thought to react at the external-facing anion transport site, EM reversibly inhibits Cl-…”
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NIP- and NAP-taurine bind to external modifier site of AE1 (band 3), at which iodide inhibits anion exchange by Knauf, P A, Spinelli, L J

“…External iodide (I-o) inhibits AE1 (band 3)-mediated anion exchange in human red blood cells by binding to a noncompetitive inhibitory site, the external…”
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Control of intracellular pH during regulatory volume decrease in HL-60 cells by Hallows, K R, Restrepo, D, Knauf, P A

“…Intracellular pH (pHi) homeostasis was investigated in human promyelocytic leukemic HL-60 cells as they undergo regulatory volume decrease (RVD) in hypotonic…”
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Functional expression of the rat anion exchanger AE2 in insect cells by a recombinant baculovirus by He, X, Wu, X, Knauf, P A, Tabak, L A, Melvin, J E

“…We used baculovirus to transiently express a rat anion exchanger (AE2) in Spodoptera frugiperda (Sf9) insect cells. No detectable Cl(-)-HCO3- exchange activity…”
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Eosin-5-maleimide inhibits red cell Cl- exchange at a noncompetitive site that senses band 3 conformation by Knauf, P A, Strong, N M, Penikas, J, Wheeler, Jr, R B, Liu, S Q

“…Eosin-5-maleimide (EM) has been used as a fluorescent probe for the external-facing transport site of the human erythrocyte band 3 protein. Changes in chloride…”
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Synthetic Diacylglycerols Trigger an Increase of Intracellular Free Calcium in Promyelocytic HL60 Cells by Restrepo, D, Kozody, D J, Knauf, P A

“…Phosphoinositide turnover is known to play an important role in intracellular free calcium homeostasis through the inositol trisphosphate-mediated release of…”
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WW-781, a potent reversible inhibitor of red cell Cl- flux, binds to band 3 by a two-step mechanism by Raha, N M, Spinelli, L J, Knauf, P A

“…WW-781 ([3-methyl-1-p-sulfophenyl-5-pyrazolone-(4)]-[1,3-dibutylbarbit uric acid]-pentamethine oxonol), a fluorescent dye that has been used for measuring…”
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Location of the chloride self-inhibitory site of the human erythrocyte anion exchange system by Knauf, P A, Mann, N A

“…Experiments were performed with intact human red blood cells to determine whether the inhibitory effects of high Cl- concentrations on Cl- exchange are…”
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Flufenamic acid senses conformation and asymmetry of human erythrocyte band 3 anion transport protein by Knauf, P A, Spinelli, L J, Mann, N A

“…With Cl as substrate, the human red blood cell anion transport (band 3) protein can exist in four conformations: Ei, with the transport site facing the…”
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Kinetics of DIDS inhibition of HL-60 cell anion exchange rules out ping-pong model with slippage by Restrepo, D, Cronise, B L, Snyder, R B, Spinelli, L J, Knauf, P A

“…According to the ping-pong model of band 3-mediated anion exchange, the transport protein has a single transport site, which can exist in either an…”
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Decreased intracellular Na+ concentration is an early event in murine erythroleukemic cell differentiation by Lannigan, D A, Knauf, P A

“…A decrease in Na+/K+-pump activity is an early event of Friend murine erythroleukemic (MEL) cell differentiation along the erythroid pathway. This decreased…”
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The relationship between anion exchange and net anion flow across the human red blood cell membrane by Knauf, P A, Fuhrmann, G F, Rothstein, S, Rothstein, A

“…The conductive (net) anion permeability of human red blood cells was determined from net KCl or K2SO4 effluxes into low K+ media at high valinomycin…”
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Cl-Cl exchange in promyelocytic HL-60 cells follows simultaneous rather than ping-pong kinetics by Restrepo, D, Kozody, D J, Spinelli, L J, Knauf, P A

“…The intra- and extracellular chloride concentration dependencies of the rate of Cl-Cl exchange in human promyelocytic leukemic HL-60 cells were studied by…”
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Changes in Na+-H+ exchange regulation upon granulocytic differentiation of HL60 cells by Restrepo, D, Kozody, D J, Knauf, P A

“…The changes in activation of Na+-H+ exchange on granulocytic differentiation of human leukemic promyelocytic HL60 cells have been studied by measuring changes…”
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