Search Results - "Knauf, P."

Development of an spFRET method to measure structure changes in ion exchange proteins by Lesoine, J. F., Holmberg, B., Maloney, P., Wang, X., Novotny, L., Knauf, P. A.

“…Aim:  Understanding the mechanism of tightly coupled ion exchange proteins, important effectors of cell volume regulation and other physiologically important…”
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Acute cell volume changes in anisotonic media affect F-actin content of HL-60 cells by Hallows, K R, Packman, C H, Knauf, P A

“…To investigate the possible role of the cytoskeleton in volume regulatory responses of human promyelocytic leukemic (HL-60) cells, we monitored and modulated…”
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Swelling-activated amino acid efflux in the human neuroblastoma cell line CHP-100 by Basavappa, S, Huang, C C, Mangel, A W, Lebedev, D V, Knauf, P A, Ellory, J C

“…1. The effects of hypoosmotic stress on cell volume and amino acid efflux were evaluated in the human neuroblastoma cell line CHP-100 with the Coulter Counter…”
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Volume-activated chloride channels in HL-60 cells: potent inhibition by an oxonol dye by Arreola, J, Hallows, K R, Knauf, P A

“…When swollen in hypotonic media, HL-60 cells exhibit a regulatory volume decrease (RVD) response as a result of net losses of K+ and Cl-. This is primarily…”
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An oxonol dye is the most potent known inhibitor of band 3-mediated anion exchange by Knauf, P A, Law, F Y, Hahn, K

“…When cells are acutely exposed to the oxonol dye, bis(1,3-dibutylbarbituric acid)pentamethine oxonol (diBA), at 0 degrees C, the concentration that gives half…”
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Regulatory volume decrease in HL-60 cells: importance of rapid changes in permeability of Cl- and organic solutes by Hallows, K R, Knauf, P A

“…Results obtained through the use of inhibitors and isotope flux and equilibration techniques indicate that the regulatory volume decrease (RVD) response of…”
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Lys-430, site of irreversible inhibition of band 3 Cl- flux by eosin-5-maleimide, is not at the transport site by Liu, S Q, Knauf, P A

“…Although eosin-5-maleimide (EM) covalently labels band 3 and has been thought to react at the external-facing anion transport site, EM reversibly inhibits Cl-…”
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Susceptibility of corneal and conjunctival pathogens to ciprofloxacin by Knauf, H P, Silvany, R, Southern, Jr, P M, Risser, R C, Wilson, S E

“…Ciprofloxacin 0.3% ophthalmic solution has been shown to be effective in the treatment of bacterial keratitis and conjunctivitis, and many physicians use…”
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NIP- and NAP-taurine bind to external modifier site of AE1 (band 3), at which iodide inhibits anion exchange by Knauf, P A, Spinelli, L J

“…External iodide (I-o) inhibits AE1 (band 3)-mediated anion exchange in human red blood cells by binding to a noncompetitive inhibitory site, the external…”
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Control of intracellular pH during regulatory volume decrease in HL-60 cells by Hallows, K R, Restrepo, D, Knauf, P A

“…Intracellular pH (pHi) homeostasis was investigated in human promyelocytic leukemic HL-60 cells as they undergo regulatory volume decrease (RVD) in hypotonic…”
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Functional expression of the rat anion exchanger AE2 in insect cells by a recombinant baculovirus by He, X, Wu, X, Knauf, P A, Tabak, L A, Melvin, J E

“…We used baculovirus to transiently express a rat anion exchanger (AE2) in Spodoptera frugiperda (Sf9) insect cells. No detectable Cl(-)-HCO3- exchange activity…”
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35Cl nuclear magnetic resonance line broadening shows that eosin-5-maleimide does not block the external anion access channel of band 3 by Liu, D., Kennedy, S.D., Knauf, P.A.

“…It has been suggested that Lys-430 of band 3, with which eosin-5-maleimide (EM) reacts, is located in the external channel through which anions gain access to…”
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Fuchs' Corneal Endothelial Cells Transduced with the Human Papilloma Virus E6/E7 Oncogenes by HE, YU-GUANG, WENG, JIAN, LI, QIAN, KNAUF, HERBERT P., WILSON, STEVEN E.

“…We sought to develop corneal endothelial cell cultures with extended lifespan from corneas with Fuchs' dystrophy. Descemet's-endothelial cell explants from…”
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Hypotonicity induces L-selectin shedding in human neutrophils by Kaba, N K, Knauf, P A

“…Expression levels of adhesion molecules on neutrophils are affected under various conditions, including ischemia, possibly because of associated increases in…”
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Eosin-5-maleimide inhibits red cell Cl- exchange at a noncompetitive site that senses band 3 conformation by Knauf, P A, Strong, N M, Penikas, J, Wheeler, Jr, R B, Liu, S Q

“…Eosin-5-maleimide (EM) has been used as a fluorescent probe for the external-facing transport site of the human erythrocyte band 3 protein. Changes in chloride…”
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WW-781, a potent reversible inhibitor of red cell Cl- flux, binds to band 3 by a two-step mechanism by Raha, N M, Spinelli, L J, Knauf, P A

“…WW-781 ([3-methyl-1-p-sulfophenyl-5-pyrazolone-(4)]-[1,3-dibutylbarbit uric acid]-pentamethine oxonol), a fluorescent dye that has been used for measuring…”
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Changes in cytoskeletal actin content, F-actin distribution, and surface morphology during HL-60 cell volume regulation by Hallows, Kenneth R., Law, Foon-Yee, Packman, Charles H., Knauf, Philip A.

“…Cell volume regulation occurs via the regulated fluxes of ions and solutes across the cell membrane in response to cell volume perturbations under anisotonic…”
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Synthetic Diacylglycerols Trigger an Increase of Intracellular Free Calcium in Promyelocytic HL60 Cells by Restrepo, D, Kozody, D J, Knauf, P A

“…Phosphoinositide turnover is known to play an important role in intracellular free calcium homeostasis through the inositol trisphosphate-mediated release of…”
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The asymmetry of chloride transport at 38 degrees C in human red blood cell membranes by Knauf, P A, Gasbjerg, P K, Brahm, J

“…Band 3-mediated Cl- exchange in human red blood cells and resealed ghosts was measured at 38 degrees C by the continuous flow tube method. When external Cl-…”
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Flufenamic acid senses conformation and asymmetry of human erythrocyte band 3 anion transport protein by Knauf, P A, Spinelli, L J, Mann, N A

“…With Cl as substrate, the human red blood cell anion transport (band 3) protein can exist in four conformations: Ei, with the transport site facing the…”
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