Search Results - "Knapp, H. F."

Precise tuning of gene expression levels in mammalian cells by Michaels, Yale S., Barnkob, Mike B., Barbosa, Hector, Baeumler, Toni A., Thompson, Mary K., Andre, Violaine, Colin-York, Huw, Fritzsche, Marco, Gileadi, Uzi, Sheppard, Hilary M., Knapp, David J. H. F., Milne, Thomas A., Cerundolo, Vincenzo, Fulga, Tudor A.

“…Precise, analogue regulation of gene expression is critical for cellular function in mammals. In contrast, widely employed experimental and therapeutic…”
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Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression by Knapp, David J. H. F., Michaels, Yale S., Jamilly, Max, Ferry, Quentin R. V., Barbosa, Hector, Milne, Thomas A., Fulga, Tudor A.

“…Spatial/temporal control of Cas9 guide RNA expression could considerably expand the utility of CRISPR-based technologies. Current approaches based on tRNA…”
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A multiplexable TALE-based binary expression system for in vivo cellular interaction studies by Toegel, Markus, Azzam, Ghows, Lee, Eunice Y., Knapp, David J. H. F., Tan, Ying, Fa, Ming, Fulga, Tudor A.

“…Binary expression systems have revolutionised genetic research by enabling delivery of loss-of-function and gain-of-function transgenes with precise…”
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Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells by Makarem, Maisam, Kannan, Nagarajan, Nguyen, Long V, Knapp, David J H F, Balani, Sneha, Prater, Michael D, Stingl, John, Raouf, Afshin, Nemirovsky, Oksana, Eirew, Peter, Eaves, Connie J

“…Many normal adult tissues contain rare stem cells with extensive self-maintaining regenerative potential. During development, the stem cells of the…”
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A blood transcriptome-based analysis of disease progression, immune regulation, and symptoms in coronavirus-infected patients by Sadanandam, Anguraj, Bopp, Tobias, Dixit, Santosh, Knapp, David J. H. F., Emperumal, Chitra Priya, Vergidis, Paschalis, Rajalingam, Krishnaraj, Melcher, Alan, Kannan, Nagarajan

“…COVID-19 patients show heterogeneity in clinical presentation and outcomes that makes pandemic control and strategy difficult; optimizing management requires a…”
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Correlates of Protective Cellular Immunity Revealed by Analysis of Population-Level Immune Escape Pathways in HIV-1 by CARLSON, Jonathan M, BRUMME, Chanson J, HAUBRICH, Richard, NELSON, George, PFEIFER, Nico, DEZIEL, Charles E, HECKERMAN, David, APPS, Richard, CARRINGTON, Mary, MALLAL, Simon, RICHARD HARRIGAN, P, JOHN, Mina, MARTIN, Eric, BRUMME, Zabrina L, LISTGARTEN, Jennifer, BROCKMAN, Mark A, LE, Anh Q, CHUI, Celia K. S, COTTON, Laura A, KNAPP, David J. H. F, RIDDLER, Sharon A

“…OA  Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+…”
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Polycomb contraction differentially regulates terminal human hematopoietic differentiation programs by Lorzadeh, A, Hammond, C, Wang, F, Knapp, D J H F, Wong, J Ch, Zhu, J Y A, Cao, Q, Heravi-Moussavi, A, Carles, A, Wong, M, Sharafian, Z, Steif, J, Moksa, M, Bilenky, M, Lavoie, P M, Eaves, C J, Hirst, M

“…Lifelong production of the many types of mature blood cells from less differentiated progenitors is a hierarchically ordered process that spans multiple cell…”
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Addendum: Precise tuning of gene expression levels in mammalian cells by Michaels, Yale S., Barnkob, Mike B., Barbosa, Hector, Baeumler, Toni A., Thompson, Mary K., Andre, Violaine, Colin-York, Huw, Fritzsche, Marco, Gileadi, Uzi, Sheppard, Hilary M., Knapp, David J. H. F., Milne, Thomas A., Cerundolo, Vincenzo, Fulga, Tudor A.

“…Following re-sequencing of the miSFIT constructs used in the paper, two of the construct variants inserted into the 3’UTR of PD-1, namely ‘12C’ and ‘17A, 18G’,…”
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The Lin28b–let-7–Hmga2 axis determines the higher self-renewal potential of fetal haematopoietic stem cells by Copley, Michael R., Babovic, Sonja, Benz, Claudia, Knapp, David J. H. F., Beer, Philip A., Kent, David G., Wohrer, Stefan, Treloar, David Q., Day, Christopher, Rowe, Keegan, Mader, Heidi, Kuchenbauer, Florian, Humphries, R. Keith, Eaves, Connie J.

“…Mouse haematopoietic stem cells (HSCs) undergo a postnatal transition in several properties, including a marked reduction in their self-renewal activity. We…”
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Discovery of a CD10-negative B-progenitor in human fetal life identifies unique ontogeny-related developmental programs by O'Byrne, Sorcha, Elliott, Natalina, Rice, Siobhan, Buck, Gemma, Fordham, Nicholas, Garnett, Catherine, Godfrey, Laura, Crump, Nicholas T., Wright, Gary, Inglott, Sarah, Hua, Peng, Psaila, Bethan, Povinelli, Benjamin, Knapp, David J.H.F., Agraz-Doblas, Antonio, Bueno, Clara, Varela, Ignacio, Bennett, Phillip, Koohy, Hashem, Watt, Suzanne M., Karadimitris, Anastasios, Mead, Adam J., Ancliff, Phillip, Vyas, Paresh, Menendez, Pablo, Milne, Thomas A., Roberts, Irene, Roy, Anindita

“…Human lymphopoiesis is a dynamic lifelong process that starts in utero 6 weeks postconception. Although fetal B-lymphopoiesis remains poorly defined, it is key…”
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Limited evolution of inferred HIV-1 tropism while viremia is undetectable during standard HAART therapy by Lee, Guinevere Q, Dong, Winnie, Mo, Theresa, Knapp, David J H F, Brumme, Chanson J, Woods, Conan K, Kanters, Steve, Yip, Benita, Harrigan, P Richard

“…HIV patients on suppressive antiretroviral therapy have undetectable viremia making it impossible to screen plasma HIV tropism if regimen change is required…”
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High-throughput analysis of single hematopoietic stem cell proliferation in microfluidic cell culture arrays by Lecault, Véronique, VanInsberghe, Michael, Sekulovic, Sanja, Knapp, David J H F, Wohrer, Stefan, Bowden, William, Viel, Francis, McLaughlin, Thomas, Jarandehei, Asefeh, Miller, Michelle, Falconnet, Didier, White, Adam K, Kent, David G, Copley, Michael R, Taghipour, Fariborz, Eaves, Connie J, Humphries, R Keith, Piret, James M, Hansen, Carl L

“…A microfluidic setup for nanoliter-volume perfused clonal culture and imaging of thousands of nonadherent cells is applied to study signaling and proliferation…”
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Single-cell analysis identifies a CD33+ subset of human cord blood cells with high regenerative potential by Knapp, David J. H. F., Hammond, Colin A., Hui, Tony, van Loenhout, Marijn T. J., Wang, Fangwu, Aghaeepour, Nima, Miller, Paul H., Moksa, Michelle, Rabu, Gabrielle M., Beer, Philip A., Pellacani, Davide, Humphries, R. Keith, Hansen, Carl, Hirst, Martin, Eaves, Connie J.

“…Elucidation of the identity and diversity of mechanisms that sustain long-term human blood cell production remains an important challenge. Previous studies…”
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Analysis of the clonal growth and differentiation dynamics of primitive barcoded human cord blood cells in NSG mice by Cheung, Alice M.S., Nguyen, Long V., Carles, Annaick, Beer, Philip, Miller, Paul H., Knapp, David J.H.F., Dhillon, Kiran, Hirst, Martin, Eaves, Connie J.

“…Human cord blood (CB) offers an attractive source of cells for clinical transplants because of its rich content of cells with sustained repopulating ability in…”
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In Vitro Selection of Clinically Relevant Bevirimat Resistance Mutations Revealed by “Deep” Sequencing of Serially Passaged, Quasispecies-Containing Recombinant HIV-1 by KNAPP, David J. H. F, HARRIGAN, P. Richard, POON, Art F. Y, BRUMME, Zabrina L, BROCKMAN, Mark, CHEUNG, Peter K

“…Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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GateFinder: projection-based gating strategy optimization for flow and mass cytometry by Aghaeepour, Nima, Simonds, Erin F, Knapp, David J H F, Bruggner, Robert V, Sachs, Karen, Culos, Anthony, Gherardini, Pier Federico, Samusik, Nikolay, Fragiadakis, Gabriela K, Bendall, Sean C, Gaudilliere, Brice, Angst, Martin S, Eaves, Connie J, Weiss, William A, Fantl, Wendy J, Nolan, Garry P

“…Abstract Motivation High-parameter single-cell technologies can reveal novel cell populations of interest, but studying or validating these populations using…”
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H3K79me2/3 controls enhancer–promoter interactions and activation of the pan-cancer stem cell marker PROM1/CD133 in MLL-AF4 leukemia cells by Godfrey, Laura, Crump, Nicholas T., O’Byrne, Sorcha, Lau, I-Jun, Rice, Siobhan, Harman, Joe R., Jackson, Thomas, Elliott, Natalina, Buck, Gemma, Connor, Christopher, Thorne, Ross, Knapp, David J. H. F., Heidenreich, Olaf, Vyas, Paresh, Menendez, Pablo, Inglott, Sarah, Ancliff, Philip, Geng, Huimin, Roberts, Irene, Roy, Anindita, Milne, Thomas A.

“…MLL gene rearrangements (MLLr) are a common cause of aggressive, incurable acute lymphoblastic leukemias (ALL) in infants and children, most of which originate…”
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Disruption of IKAROS activity in primitive chronic-phase CML cells mimics myeloid disease progression by Beer, Philip A., Knapp, DavidJ.H.F., Miller, Paul H., Kannan, Nagarajan, Sloma, Ivan, Heel, Kathy, Babovic, Sonja, Bulaeva, Elizabeth, Rabu, Gabrielle, Terry, Jefferson, Druker, Brian J., Loriaux, Marc M., Loeb, Keith R., Radich, Jerald P., Erber, Wendy N., Eaves, Connie J.

“…Without effective therapy, chronic-phase chronic myeloid leukemia (CP-CML) evolves into an acute leukemia (blast crisis [BC]) that displays either myeloid or…”
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Surface potential mapping: A qualitative material contrast in SPM by Jacobs, H.O., Knapp, H.F., Müller, S., Stemmer, A.

“…Electric potential measurements on different metals and semiconductors have been performed using a scanning probe microscope. The measured potential shows a…”
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"Deep" sequencing accuracy and reproducibility using Roche/454 technology for inferring co-receptor usage in HIV-1 by Knapp, David J H F, McGovern, Rachel A, Poon, Art F Y, Zhong, Xiaoyin, Chan, Dennison, Swenson, Luke C, Dong, Winnie, Harrigan, P Richard

“…Next generation, "deep", sequencing has increasing applications both clinically and in disparate fields of research. This study investigates the accuracy and…”
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