Search Results - "Klumb, Lisa"

Biomimicry as a route to new materials: what kinds of lessons are useful? by Reed, Emily J, Klumb, Lisa, Koobatian, Maxwell, Viney, Christopher

“…We consider the attributes of a successful engineered material, acknowledging the contributions of composition and processing to properties and performance. We…”
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Molecular Recognition between Genetically Engineered Streptavidin and Surface-Bound Biotin by Pérez-Luna, Víctor H, O'Brien, Michael J, Opperman, Kimberly A, Hampton, Philip D, López, Gabriel P, Klumb, Lisa A, Stayton, Patrick S

“…This study examines the binding of wild-type streptavidin and streptavidin mutants to biotin-terminated self-assembled monolayers (SAMs) as a model of…”
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Energetic Roles of Hydrogen Bonds at the Ureido Oxygen Binding Pocket in the Streptavidin−Biotin Complex by Klumb, Lisa A, Chu, Vano, Stayton, Patrick S

“…The high-affinity streptavidin−biotin complex is characterized by an extensive hydrogen-bonding network. A study of hydrogen-bonding energetics at the ureido…”
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Structural studies of the streptavidin binding loop by Stenkamp, Ronald E., Trong, Isolde Le, Klumb, Lisa, Stayton, Patrick S., Freitag, Stefanie

“…The streptavidin‐biotin complex provides the basis for many important biotechnological applications and is an interesting model system for studying…”
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Poly(lactide-co-glycolide) microsphere formulations of darbepoetin alfa: spray drying is an alternative to encapsulation by spray-freeze drying by Burke, Paul A, Klumb, Lisa A, Herberger, John D, Nguyen, Xichdao C, Harrell, Roy A, Zordich, Monica

“…The purpose of this work was to evaluate spray-freeze drying and spray drying processes for encapsulation of darbepoetin alfa (NESP, Aranesp). Darbepoetin alfa…”
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Molecular engineering of proteins and polymers for targeting and intracellular delivery of therapeutics by Stayton, Patrick S, Hoffman, Allan S, Murthy, Niren, Lackey, Chantal, Cheung, Charles, Tan, Philip, Klumb, Lisa A, Chilkoti, Ashutosh, Wilbur, F.Scott, Press, Oliver W

“…There are many protein and DNA based therapeutics under development in the biotechnology and pharmaceutical industries. Key delivery challenges remain before…”
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A Structural Snapshot of an Intermediate on the Streptavidin-Biotin Dissociation Pathway by Freitag, Stefanie, Chu, Vano, Penzotti, Julie E., Klumb, Lisa A., To, Richard, Hyre, David, Le Trong, Isolde, Lybrand, Terry P., Stenkamp, Ronald E., Stayton, Patrick S.

“…It is currently unclear whether small molecules dissociate from a protein binding site along a defined pathway or through a collection of dissociation…”
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Theoretical and Experimental Studies of Biotin Analogues That Bind Almost as Tightly to Streptavidin as Biotin by Dixon, Richard W, Radmer, Randall J, Kuhn, Bernd, Kollman, Peter A, Yang, Jaemoon, Raposo, Cesar, Wilcox, Craig S, Klumb, Lisa A, Stayton, Patrick S, Behnke, Craig, Le Trong, Isolde, Stenkamp, Ronald

“…We have used a newly developed qualitative computational approach, PROFEC (Pictorial Representation of Free Energy Changes), to visualize the areas of the…”
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Antibody Fragments in Tumor Pretargeting. Evaluation of Biotinylated Fab‘ Colocalization with Recombinant Streptavidin and Avidin by Wilbur, D. Scott, Hamlin, Donald K, Vessella, Robert L, Stray, James E, Buhler, Kent R, Stayton, Patrick S, Klumb, Lisa A, Pathare, Pradip M, Weerawarna, S. Ananda

“…An evaluation of the use of a biotinylated monoclonal antibody Fab‘ fragment in tumor pretargeting was conducted. As a model system, tumor colocalization of…”
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Effects of Interfacial Binding Kinetics on Two-Dimensional Streptavidin Crystallization by Koppenol, Sandy, Klumb, Lisa A, Vogel, Viola, Stayton, Patrick S

“…The two-dimensional crystallization of streptavidin at biotinylated interfaces provides a model system for elucidating the role of interfacial binding dynamics…”
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Structural studies of hydrogen bonds in the high-affinity streptavidin-biotin complex: mutations of amino acids interacting with the ureido oxygen of biotin by Le Trong, Isolde, Freitag, Stefanie, Klumb, Lisa A., Chu, Vano, Stayton, Patrick S., Stenkamp, Ronald E.

“…An elaborate hydrogen‐bonding network contributes to the tight binding of biotin to streptavidin. The specific energetic contributions of hydrogen bonds to the…”
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Streptavidin in Antibody Pretargeting. Comparison of a Recombinant Streptavidin with Two Streptavidin Mutant Proteins and Two Commercially Available Streptavidin Proteins by Wilbur, D. Scott, Stayton, Patrick S, To, Richard, Buhler, Kent R, Klumb, Lisa A, Hamlin, Donald K, Stray, James E, Vessella, Robert L

“…In this investigation, a comparison of wild type recombinant streptavidin (r-SAv) with two genetically engineered mutant r-SAv proteins was undertaken. The…”
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Streptavidin–biotin binding energetics by Stayton, Patrick S, Freitag, Stefanie, Klumb, Lisa A, Chilkoti, Ashutosh, Chu, Vano, Penzotti, Julie E, To, Richard, Hyre, David, Le Trong, Isolde, Lybrand, Terry P, Stenkamp, Ronald E

“…The high affinity energetics in the streptavidin–biotin system provide an excellent model system for studying how proteins balance enthalpic and entropic…”
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Development of new biotin/streptavidin reagents for pretargeting by Scott Wilbur, D, Pathare, Pradip M, Hamlin, Donald K, Stayton, Patrick S, To, Richard, Klumb, Lisa A, Buhler, Kent R, Vessella, Robert L

“…The high affinity of biotin for streptavidin has made this pair of molecules very useful for in vivo applications. To optimize reagents for one potential in…”
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X-ray crystallographic studies of streptavidin mutants binding to biotin by Freitag, Stefanie, Le Trong, Isolde, Klumb, Lisa A, Chu, Vano, Chilkoti, Ashutosh, Stayton, Patrick S, Stenkamp, Ronald E

“…On the basis of high resolution crystallographic studies ot streptavidin and its biotin complex, three principal binding motifs have been identified that…”
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Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex by Freitag, S, Le Trong, I, Chilkoti, A, Klumb, L A, Stayton, P S, Stenkamp, R E

“…Previous thermodynamic and computational studies have pointed to the important energetic role of aromatic contacts in generating the exceptional binding free…”
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Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex 1 1Edited by I. A. Wilson by Freitag, Stefanie, Le Trong, Isolde, Chilkoti, Ashutosh, Klumb, Lisa A, Stayton, Patrick S, Stenkamp, Ronald E

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Design of a glucose-sensitive insulin delivery system by Klumb, Lisa A

“…In this research, the glucose sensitive membrane was examined in different design configurations for use as an insulin delivery system. The glucose sensitive…”
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Atomic resolution structure of biotin-free Tyr43Phe streptavidin: what is in the binding site? by Freitag, Stefanie, Le Trong, Isolde, Klumb, Lisa A., Stayton, Patrick S., Stenkamp, Ronald E.

“…The streptavidin–biotin system is an example of a high‐affinity protein–ligand pair (Ka≃ 1013 mol−1). The thermodynamic and structural properties have been…”
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