New ionic liquids based on 5-fluorouracil: Tuning of BSA binding and cytotoxicity

In this work, we describe the synthesis, interactions with bovine serum albumin, and cytotoxicity of new ionic liquids based on 5-fluorouracil (API-ILs) with different cations (imidazolium, choline, isoquinolinium, guanidinium). The secondary and tertiary structure of BSA in solutions with different...

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Bibliographic Details
Published in:	International journal of biological macromolecules Vol. 257; no. Pt 1; p. 128642
Main Authors:	Khachatrian, Artashes A., Mukhametzyanov, Timur A., Salikhov, Ramazan Z., Klimova, Alexandra E., Gafurov, Zufar N., Kantyukov, Artyom O., Yakhvarov, Dmitry G., Garifullin, Bulat F., Mironova, Diana A., Voloshina, Alexandra D., Solomonov, Boris N.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-02-2024
Subjects:	Binding Cytotoxicity Ionic liquid Protein Structure Binding Cytotoxicity Structure Protein Ionic liquid
Online Access:	Get full text
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Summary:	In this work, we describe the synthesis, interactions with bovine serum albumin, and cytotoxicity of new ionic liquids based on 5-fluorouracil (API-ILs) with different cations (imidazolium, choline, isoquinolinium, guanidinium). The secondary and tertiary structure of BSA in solutions with different concentrations of API-ILs was monitored by the circular dichroism (CD) technique. The addition of API-ILs does not lead to structural changes in BSA. A quenching of fluorescence spectra intensity of BSA in presence of all API-ILs was observed, allowing the quantification of binding between API-ILs and BSA. The preferred localization of both ions in API-ILs differs significantly depending on the structure of the cation according to molecular docking. The aggregation of BSA in presence of API-ILs was analyzed by the dynamic light scattering (DLS) method, revealing a moderate increase in particle size. Cytotoxicity and selectivity of API-ILs on cancer and normal cell lines were estimated, showing a clear modification of the pharmaceutic activity of ionic liquid compared to 5-fluorouracil.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0141-8130 1879-0003
DOI:	10.1016/j.ijbiomac.2023.128642