Identification of a unique group of human papillomavirus type 16 sequence variants among clinical isolates from Barbados

1 Human DNA Virus Laboratory, Department of Virology, Academic Medical Centre, University of Amsterdam, Amsterdam 2 Laboratory of Virology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands and 3 Department of Pathology and Surgery, University of West Indie...

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Bibliographic Details
Published in:	Journal of general virology Vol. 75; no. 9; pp. 2457 - 2462
Main Authors:	Smits, Henk L, Traanberg, Kristine F, Krul, Mark R. L, Prussia, Patsy R, Kuiken, Carla L, Jebbink, Maarten F, Kleyne, John A. F. W, van den Berg, Rocco H, Capone, Bruno, de Bruyn, Anna, ter Schegget, Jan
Format:	Journal Article
Language:	English
Published:	Reading Soc General Microbiol 01-09-1994 Society for General Microbiology
Subjects:	Asia Barbados Base Sequence Biological and medical sciences DNA Primers Ethnic Groups Europe Fundamental and applied biological sciences. Psychology Genetic Variation Humans Microbiology Molecular Sequence Data Mutation Netherlands Oncogene Proteins, Viral - genetics Open Reading Frames Papillomaviridae - genetics Papillomaviridae - isolation & purification Papillomavirus E7 Proteins Phylogeny Polymerase Chain Reaction - methods Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Virology Central America Barbados America West Indies Netherlands Asia Europe Papillomavirus Virus Human Human papillomavirus 16 Phylogenetic tree Genetic variant Molecular epidemiology Papovaviridae Mutation Clinical isolate
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Summary:	1 Human DNA Virus Laboratory, Department of Virology, Academic Medical Centre, University of Amsterdam, Amsterdam 2 Laboratory of Virology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands and 3 Department of Pathology and Surgery, University of West Indies, Cave Hill Campus, Barbados The naturally occurring sequence variation of human papillomavirus type 16 (HPV-16) was analysed by direct sequence analysis of the PCR products of the long control region (LCR), the E5 and E7 open reading frames (ORFs), a segment of the L2 ORF overlapping the early viral poly(A) signal and a small segment of the L1 ORF or clinical isolates from Barbados and The Netherlands. Despite the widely different geographical and ethnic origin of the two groups of specimens, sequence analysis revealed relatively few mutational differences. Analysis of the LCR and the E5 ORF appeared to be the minimum requirement for the correct positioning of these variants in the HPV-16 phylogenetic tree. Most of the Barbadian variants appeared to be located at a unique position in the HPV-16 phylogenetic tree, at the internal branch close to the point where the European and Asian branches diverge. In contrast, most of the Dutch samples were located on the European branch. Present address: Royal Tropical Institute, N. H. Swellengrebel Laboratory of Tropical Hygiene, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands. Received 26 January 1994; accepted 8 April 1994.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0022-1317 1465-2099
DOI:	10.1099/0022-1317-75-9-2457