Expression of a unique plastid-localized heat-shock protein is genetically linked to acquired thermotolerance in wheat

We have used a combination of molecular and classical genetic approaches to delineate the relationship between a specific HSP member and cell viability under heat stress. Using recombinant inbred lines (RILs) of wheat, derived from a cross of the thermotolerant cultivar 'Mustang' and the t...

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Bibliographic Details
Published in:	Theoretical and applied genetics Vol. 95; no. 5/6; pp. 834 - 841
Main Authors:	Joshi, C.P, Kleuva, N.Y, Morrow, K.J, Nguyen, H.T
Format:	Journal Article
Language:	English
Published:	Heidelberg Springer 01-10-1997 Berlin Springer Nature B.V
Subjects:	acquired characters amino acid sequences Antisense DNA Biological and medical sciences Classical genetics, quantitative genetics, hybrids complementary DNA cultivars Fundamental and applied biological sciences. Psychology genbank/l41503 genbank/l41504 gene expression Genetic aspects Genetics Genetics of eukaryotes. Biological and molecular evolution Heat shock proteins heat stress heat tolerance Identification and classification inbred lines leaves linkage (genetics) messenger RNA molecular sequence data nucleotide sequences Plant defenses Plant genetics plastids protein composition protein synthesis Proteins Pteridophyta, spermatophyta recombinant inbred lines Triticum aestivum Vegetals United States Heat resistance Monocotyledones Nucleotide sequence Homology Gene expression Cereal crop Gene Gramineae Thermal shock Angiospermae Heat shock protein Spermatophyta Chloroplast Triticum aestivum
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Summary:	We have used a combination of molecular and classical genetic approaches to delineate the relationship between a specific HSP member and cell viability under heat stress. Using recombinant inbred lines (RILs) of wheat, derived from a cross of the thermotolerant cultivar 'Mustang' and the thermosusceptible cultivar 'Sturdy,' we have identified a unique HSP and a differentially expressed cDNA sequence, both related to the plastic-localized HSP26 gene family. that are closely associated with acquired thermotolerance in wheat. An isoform of HSP26 was synthesized under heat stress in all examined thermotolerant RILs and 'Mustang', and was absent in all examined thermosusceptible RILs and 'Sturdy.' Using a modified differential-display method, we have also identified a gene-specific cDNA sequence that is similar to other known members of the wheat HSP26 gene family and is selectively expressed in 'Mustang' and most of the examined thermotolerant RILs, but not expressed in 'Sturdy' and all the thermosusceptible RILs. These results suggest a genetic linkage between the acquired thermotolerance trait and the differential expression of a unique member of the HSP26 gene family.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0040-5752 1432-2242
DOI:	10.1007/s001220050633