Search Results - "Klösgen, Ralf Bernd"
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The exception proves the rule? Dual targeting of nuclear-encoded proteins into endosymbiotic organelles
Published in The New phytologist (01-01-2014)“…Plant cells harbor two types of endosymbiotic organelle: mitochondria and chloroplasts. As a consequence of endosymbiotic gene transfer, the majority of their…”
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2
Simultaneous isolation of intact mitochondria and chloroplasts from a single pulping of plant tissue
Published in Journal of plant physiology (15-05-2010)“…Isolated organelles are suitable tools for the investigation of organelle function. However, if the properties of different organelles are to be compared,…”
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3
A high‐definition native polyacrylamide gel electrophoresis system for the analysis of membrane complexes
Published in The Plant journal : for cell and molecular biology (01-07-2011)“…Summary Native polyacrylamide gel electrophoresis (PAGE) is an important technique for the analysis of membrane protein complexes. A major breakthrough was the…”
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Targeting specificity of nuclear-encoded organelle proteins with a self-assembling split-fluorescent protein toolkit
Published in Journal of cell science (01-06-2019)“…A large number of nuclear-encoded proteins are targeted to the organelles of endosymbiotic origin, namely mitochondria and plastids. To determine the targeting…”
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5
Inactivation of a Predicted Leader Peptidase Prevents Photoautotrophic Growth of Synechocystis sp. Strain PCC 6803
Published in Journal of Bacteriology (01-05-2005)“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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6
Preparation of physiologically active inside-out vesicles from plant inner mitochondrial membranes
Published in Frontiers in plant science (27-06-2023)“…For many metabolites, the major barrier between cytosol and mitochondrial matrix is the inner membrane of mitochondria, the site of the respiratory electron…”
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Dual or Not Dual?-Comparative Analysis of Fluorescence Microscopy-Based Approaches to Study Organelle Targeting Specificity of Nuclear-Encoded Plant Proteins
Published in Frontiers in plant science (19-09-2018)“…Plant cells are unique as they carry two organelles of endosymbiotic origin, namely mitochondria and chloroplasts (plastids) which have specific but partially…”
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Purification of rabbit serum histidine-proline-rich glycoprotein via preparative gel electrophoresis and characterization of its glycosylation patterns
Published in PloS one (21-09-2017)“…Histidine-Proline-rich Glycoprotein (HPRG) is a plasma protein of vertebrates and several marine bivalves. Due to its multidomain structure consisting of…”
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9
How to achieve Tat transport with alien TatA
Published in Scientific reports (18-08-2017)“…TatA is an essential and structurally conserved component of all known Twin-arginine transport (Tat) machineries which are able to catalyse membrane transport…”
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10
C-terminal truncation of a Tat passenger protein affects its membrane translocation by interfering with receptor binding
Published in Biological chemistry (01-04-2015)“…During thylakoid transport of the chimeric model twin-arginine translocation (Tat) substrate 16/23, two consecutive translocation intermediates with different…”
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The Tat pathway in bacteria and chloroplasts (review)
Published in Molecular membrane biology (01-01-2005)“…Both in prokaryotic organisms and in chloroplasts, a specialized protein transport pathway exists which is capable of translocating proteins in a fully folded…”
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12
Twin arginine translocation (Tat)-dependent protein transport: the passenger protein participates in the initial membrane binding step
Published in Biological chemistry (01-12-2010)“…The initial step in twin arginine translocation (Tat)-dependent thylakoid transport of the 16/23 chimera is the interaction of the protein with the lipid…”
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13
A Stromal Pool of TatA Promotes Tat-dependent Protein Transport across the Thylakoid Membrane
Published in The Journal of biological chemistry (05-12-2008)“…In chloroplasts and bacteria, the Tat (twin-arginine translocation) system is engaged in transporting folded passenger proteins across the thylakoid and…”
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14
Cellular localization of dual positional specific maize lipoxygenase-1 in transgenic rice and calcium-mediated membrane association
Published in Plant science (Limerick) (01-09-2011)“…[Display omitted] ▶ The maize LOX1 is localized in cytoplasm in TR rice. ▶ The plastid membrane association of maize LOX1 is mediated by Ca 2+ in vitro. ▶ The…”
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15
Dual Targeting of a Mitochondrial Protein: The Case Study of Cytochrome C1
Published in Molecular plant (01-07-2011)“…As a result of the endosymbiotic gene transfer, the majority of proteins of mitochondria and chloroplasts is encoded in the nucleus and synthesized in the…”
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16
New insights on stromules: Stroma filled tubules extended by independent plastids
Published in Plant signaling & behavior (01-09-2012)“…The recognition of stromules as sporadically extended stroma filled tubules from all kinds of plastids constitutes one of the major insights that resulted from…”
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17
Diversity in Degrees of Freedom of Mitochondrial Transit Peptides
Published in Molecular biology and evolution (01-08-2009)“…Most mitochondrial proteins are synthesized in the cytosol of eukaryotic cells as precursor proteins carrying N-terminal extensions called transit peptides or…”
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18
In vivo transport of folded EGFP by the ΔpH/TAT-dependent pathway in chloroplasts of Arabidopsis thaliana
Published in Journal of experimental botany (01-08-2004)“…Among the protein translocation pathways of the thylakoid membrane in chloroplasts, the ΔpH/TAT pathway is unique in several aspects. In vitro transport assays…”
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Toc, Tic, Tat et al.: structure and function of protein transport machineries in chloroplasts
Published in Journal of plant physiology (01-02-2006)“…The chloroplast is an organelle of prokaryotic origin that is situated in an eukaryotic cellular environment. As a result of this formerly endosymbiotic…”
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20
Functional reconstitution of TatB into the thylakoidal Tat translocase
Published in Biochimica et biophysica acta. Molecular cell research (01-02-2020)“…We have established an experimental system for the functional analysis of thylakoidal TatB, a component of the membrane-integral TatBC receptor complex of the…”
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