Search Results - "Klösgen, R.B."

new type of signal peptide: central role of a twin-arginine motif in transfer signals for the delta pH-dependent thylakoidal protein translocase by Chaddock, A. M, Mant, A, Karnauchov, I, Brink, S, Herrmann, R.G, Klosgen, R.B, Robinson, C

“…The delta pH-driven and Sec-related thylakoidal protein translocases recognise distinct types of thylakoid transfer signal, yet all transfer signals resemble…”
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Protein import into chloroplasts. The hydrophilic lumenal proteins exhibit unexpected import and sorting specificities in spite of structurally conserved transit peptides by CLAUSMEYER, S, KLÖSGEN, R. B, HERRMANN, R. G

“…Plastocyanin and the 16-, 23-, and 33-kDa polypeptides of the oxygen-evolving complex associated with photosystem II are hydrophilic, nuclear encoded…”
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Spm (En) transposable element controls the excision of a 2-kb DNA insert at the wxm-8 allele of Zea mays by Schwarz-Sommer, Zs, Gierl, A, Klosgen, R.B, Wienand, U, Peterson, P.A

“…The waxy (Wx) locus of Zea mays was cloned from strains carrying the wild‐type and wxm‐8 mutant alleles. The receptor component of the Suppressor‐Mutator (Spm)…”
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Mutations at the stromal processing peptidase cleavage site of a thylakoid lumen protein precursor affect the rate of processing but not the fidelity by Bassham, D.C, Creighton, A.M, Karnauchov, I, Herrmann, R.G, Klosgen, R.B, Robinson, C

“…Nuclear-encoded stromal proteins are imported into the chloroplast by means of presequences, or transit peptides, which are removed after import by a stromal…”
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Electron transfer from plastocyanin to photosystem I by Haehnel, W., Jansen, T., Gause, K., Klösgen, R.B., Stahl, B., Michl, D., Huvermann, B., Karas, M., Herrmann, R.G.

“…Mutant plastocyanins with Leu at position 10, 90 or 83 (Gly, Ala and Tyr respectively in wildtype) were constructed by site‐specific mutagenesis of the spinach…”
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Targeting of proteins to the thylakoids by bipartite presequences: CFoII is imported by a novel, third pathway by Michl, D., Robinson, C., Shackleton, J.B., Herrmann, R.G., Klösgen, R.B.

“…The CFoII subunit of the ATP synthase is an integral component of the thylakoid membrane which is synthesized in the cytosol with a bipartite, lumen‐targeting…”
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The presequence of a chimeric construct dictates which of two mechanisms are utilized for translocation across the thylakoid membrane: evidence for the existence of two distinct translocation systems by Robinson, C., Cai, D., Hulford, A., Brock, I.W., Michl, D., Hazell, L., Schmidt, I., Herrmann, R.G., Klösgen, R.B.

“…The translocation of plastocyanin across the thylakoid membrane in Pisum sativum has been studied in reconstitution assays and using chimeric constructs. The…”
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Toc, Tic, Tat et al.: structure and function of protein transport machineries in chloroplasts by Gutensohn, Michael, Fan, Enguo, Frielingsdorf, Stefan, Hanner, Peter, Hou, Bo, Hust, Bianca, Klösgen, Ralf Bernd

“…The chloroplast is an organelle of prokaryotic origin that is situated in an eukaryotic cellular environment. As a result of this formerly endosymbiotic…”
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Protein translocation across the thylakoid membrane-a tale of two mechanisms (FEBS 125020) by Robinson, C., Klösgen, R.B., Herrmann, R.G., Shackleton, J.B.

“…In vitro reconstitution assays have been used in recent years to probe the mechanisms by which a variety of cytosolically synthesised proteins are transported…”
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Targeting of proteins into and across the thylakoid membrane--a multitude of mechanisms by Robinson, C, Klosgen, R.B

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In vivo transport of folded EGFP by the deltapH/TAT-dependent pathway in chloroplasts of Arabidopsis thaliana by Marques, J.P, Schattat, M.H, Hause, G, Dudeck, I, Klosgen, R.B

“…Among the protein translocation pathways of the thylakoid membrane in chloroplasts, the deltapH/TAT pathway is unique in several aspects. In vitro transport…”
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The Spm (En) transposable element controls the excision of a 2-kb DNA insert at the wx m-8 allele of Zea mays by Schwarz-Sommer, Zs, Gierl, A., Klösgen, R.B., Wienand, U., Peterson, P.A., Saedler, H.

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Protease-sensitive thylakoidal import machinery for the Sec-, deltapH- and signal recognition particle-dependent protein targeting pathways, but not for CFoII integration by Robinson, D, Karnauchov, I, Herrmann, R.G, Klosgen, R.B, Robinson, C

“…Nuclear-encoded proteins are targeted into and across the thylakoid membrane by a surprising variety of mechanisms. Distinct Sec- and delta pH-dependent…”
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Sorting of Nuclear-encoded Chloroplast Membrane Proteins to the Envelope and the Thylakoid Membrane (∗) by Brink, Susanne, Fischer, Karsten, Klösgen, Ralf-Bernd, Flügge, Ulf-Ingo

“…The spinach triose phosphate/phosphate translocator and the 37-kDa protein are both integral components of the chloroplast inner envelope membrane. They are…”
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The role of plastids in the expression of nuclear genes for thylakoid proteins studied with chimeric beta-glucuronidase gene fusions by Bolle, C. (Botanisches Institut der Ludwig-Maximilians-Universitat, Munchen, Germany.), Sopory, S, Lubberstedt, T, Klosgen, R.B, Herrmann, R.G, Oelmuller, R

“…We have analyzed plastid and nuclear gene expression in tobacco seedlings using the carotenoid biosynthesis inhibitor norflurazon. mRNA levels for three…”
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Isolation and characterization of a cDNA encoding the SecA protein from spinach chloroplasts. Evidence for azide resistance of Sec-dependent protein translocation across thylakoid membranes in spinach by Berghofer, J. (Ludwig-Maximilians-Universitat, Munchen, Germany.), Karnauchov, I, Herrmann, R.G, Klosgen, R.B

“…Thylakoid membranes of chloroplasts in higher plants harbor different pathways for the translocation of proteins. One of these routes is related to the…”
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Targeting of proteins to the thylakoids by bipartite presequn eces: CFoll is imported by a novel, third pathway by Michl, D, Robinson, C, Shackleton, J.B, Herrmann, R.G, Klosgen, R.B

“…The CFoII subunit of the ATP synthase is an integral component of the thylakoid membrane which is synthesized in the cytosol with a bipartite, lumen-targeting…”
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20 kDa apoprotein of the CP24 complex of photosystem II: an alternative model to study import and intra-organellar routing of nuclear-encoded thylakoid proteins by Cai, D, Herrmann, G, Klosgen, R.B

“…Summary The 20 kDa polypeptide, the apoprotein of the chlorophyll a/b antenna complex CP24 associated with photosystem II, is a remote relative of…”
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Proton gradient-driven import of the 16kDa oxygen-evolving complex protein as the full precursor protein by isolated thylakoids by Klosgen, R.B, Brock, I.W, Herrmann, R.G, Robinson, C

“…The biogenesis of the lumenal 16 kDa protein of the photosynthetic oxygen-evolving complex was analysed using an assay for the import of proteins by isolated…”
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Transport of CptA protein from the cyanobacterium Synechocystis 6803 across the thylakoid membrane in chloroplasts by Karnauchov, I, Herrmann, R.G, Pakrasi, H.B, Klosgen, R.B

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