Search Results - "Kjeldsen, Thomas B."

Enhanced disulphide bond stability contributes to the once-weekly profile of insulin icodec by Hubálek, František, Cramer, Christian N., Helleberg, Hans, Johansson, Eva, Nishimura, Erica, Schluckebier, Gerd, Steensgaard, Dorte Bjerre, Sturis, Jeppe, Kjeldsen, Thomas B.

“…Insulin icodec is a once-weekly insulin analogue that has a long half-life of approximately 7 days, making it suitable for once weekly dosing. The Insulin…”
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Molecular Engineering of Insulin Icodec, the First Acylated Insulin Analog for Once-Weekly Administration in Humans by Kjeldsen, Thomas B, Hubálek, František, Hjørringgaard, Claudia U, Tagmose, Tina M, Nishimura, Erica, Stidsen, Carsten E, Porsgaard, Trine, Fledelius, Christian, Refsgaard, Hanne H. F, Gram-Nielsen, Sanne, Naver, Helle, Pridal, Lone, Hoeg-Jensen, Thomas, Jeppesen, Claus Bekker, Manfè, Valentina, Ludvigsen, Svend, Lautrup-Larsen, Inger, Madsen, Peter

“…Here, we describe the molecular engineering of insulin icodec to achieve a plasma half-life of 196 h in humans, suitable for once-weekly subcutaneously…”
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Predicting human half-life for insulin analogs: An inter-drug approach by Bendtsen, Kristian M., Harder, Magnus W.H., Glendorf, Tine, Kjeldsen, Thomas B., Kristensen, Niels R., Refsgaard, Hanne H.F.

“…[Display omitted] •PK predictions.•Inter-drug approach.•Comparison of translation methods.•Single animal translation.•in vitro-in vivo. An inter-drug approach,…”
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Engineering of Orally Available, Ultralong-Acting Insulin Analogues: Discovery of OI338 and OI320 by Kjeldsen, Thomas B, Hubálek, František, Tagmose, Tina M, Pridal, Lone, Refsgaard, Hanne H F, Porsgaard, Trine, Gram-Nielsen, Sanne, Hovgaard, Lars, Valore, Henrik, Münzel, Martin, Hjørringgaard, Claudia U, Jeppesen, Claus Bekker, Manfè, Valentina, Hoeg-Jensen, Thomas, Ludvigsen, Svend, Nielsen, Peter Kresten, Lautrup-Larsen, Inger, Stidsen, Carsten E, Wulff, Erik M, Garibay, Patrick W, Kodra, János T, Nishimura, Erica, Madsen, Peter

“…Recently, the first basal oral insulin (OI338) was shown to provide similar treatment outcomes to insulin glargine in a phase 2a clinical trial. Here, we…”
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Intracellular Retention of Newly Synthesized Insulin in Yeast Is Caused by Endoproteolytic Processing in the Golgi Complex by Zhang, Bao-yan, Chang, Amy, Kjeldsen, Thomas B., Arvan, Peter

“…An insulin-containing fusion protein (ICFP, encoding the yeast prepro-α factor leader peptide fused via a lysine-arginine cleavage site to a single chain…”
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Molecular Engineering of Efficacious Mono-Valent Ultra-Long Acting Two-Chain Insulin-Fc Conjugates by Tagmose, Tina M, Pedersen, Karen-Margrethe, Pridal, Lone, Stidsen, Carsten E, Pedersen, Marie Ø, Lin, Zhaosheng, Zhang, Yuanyuan, Wan, Zhe, Ferreras, Mercedes, Naver, Helle, Nielsen, Peter K, Cao, Zheng, Wang, Yi, Lykke, Lennart, Christensen, Josefine L, Jensen, Victoria S, Manfè, Valentina, Pedersen, Thomas Å, Johansson, Eva, Madsen, Peter, Kodra, János T, Münzel, Martin, De Maria, Leonardo, Nishimura, Erica, Kjeldsen, Thomas B

“…Here, we describe molecular engineering of monovalent ultra-long acting two-chain insulin-Fc conjugates. Insulin-Fc conjugates were synthesized using…”
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Changes in glucose and fat metabolism in response to the administration of a hepato-preferential insulin analog by Edgerton, Dale S, Moore, Mary C, Winnick, Jason J, Scott, Melanie, Farmer, Ben, Naver, Helle, Jeppesen, Claus B, Madsen, Peter, Kjeldsen, Thomas B, Nishimura, Erica, Brand, Christian L, Cherrington, Alan D

“…Endogenous insulin secretion exposes the liver to three times higher insulin concentrations than the rest of the body. Because subcutaneous insulin delivery…”
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Peripherally delivered hepatopreferential insulin analog insulin‐406 mimics the hypoglycaemia‐sparing effect of portal vein human insulin infusion in dogs by Gregory, Justin M., Kraft, Guillaume, Scott, Melanie F., Neal, Doss W., Farmer, Ben, Smith, Marta S., Hastings, Jon R., Madsen, Peter, Kjeldsen, Thomas B., Hostrup, Susanne, Brand, Christian L., Fledelius, Christian, Nishimura, Erica, Cherrington, Alan D.

“…Aims We previously quantified the hypoglycaemia‐sparing effect of portal vs peripheral human insulin delivery. The current investigation aimed to determine…”
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The road to the first, fully active and more stable human insulin variant with an additional disulfide bond by Vinther, Tine N., Kjeldsen, Thomas B., Jensen, Knud J., Hubálek, František

“…Insulin, a small peptide hormone, is crucial in maintaining blood glucose homeostasis. The stability and activity of the protein is directed by an intricate…”
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Expression, Receptor Binding, and Biophysical Characterization of Guinea Pig Insulin desB30: A Monomeric Insulin Variant by Engholm, Ebbe, Hansen, Thomas H., Johansson, Eva, Strauss, Holger M., Vinther, Tine N., Jensen, Knud J., Hubálek, Frantisek, Kjeldsen, Thomas B.

“…Here we report, for the first time, the heterologous expression of desB30 guinea pig insulin (GI desB30) in the yeast Saccharomyces cerevisiae. The affinities…”
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Identification of Anchor Points for Chemical Modification of a Small Cysteine-Rich Protein by Using a Cysteine Scan by Vinther, Tine N., Ribel, Ulla, Åskov Pedersen, Thomas, Kjeldsen, Thomas B., Jensen, Knud J., Hubálek, František

“…Chemical modifications of proteins are increasingly important in the development of protein drugs with fine‐tuned properties. Regioselective modification, such…”
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Inside Cover: Identification of Anchor Points for Chemical Modification of a Small Cysteine-Rich Protein by Using a Cysteine Scan (ChemBioChem 16/2011) by Vinther, Tine N., Ribel, Ulla, Åskov Pedersen, Thomas, Kjeldsen, Thomas B., Jensen, Knud J., Hubálek, František

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Human-human hybridomas and human monoclonal antibodies obtained by fusion of lymph node lymphocytes from breast cancer patients by KJELDSEN, T. B, RASMUSSEN, B. B, ROSE, C, ZEUTHEN, J

“…Lymphocytes from lymph nodes obtained from breast cancer patients undergoing mastectomy were fused with the 0467.3, UC729HF2, or KR-12 human cell lines,…”
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Monoclonal antibodies reactive with components in serum-free conditioned medium from a human breast cancer cell line (MCF-7) by Kjeldsen, T B, Laursen, I, Lykkesfeldt, A, Briand, P, Zeuthen, J

“…Approximately 10,000 primary hybridomas were generated after immunization with either serum-free conditioned medium (SFCM) or extracts from the human breast…”
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