Search Results - "Kitson, Trevor M"
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Spectrophotometric and kinetic studies on the binding of the bioflavonoid quercetin to bovine serum albumin
Published in Bioscience, biotechnology, and biochemistry (01-10-2004)“…This work investigates the binding of the blofavonoid, quercetln, to bovine serum albumin (BSA) by spectrophotometric techniques involving both the…”
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Telling the Stories of Chemistry
Published in Journal of chemical education (01-10-2005)“…This article describes the author's long-established practice of including in every chemistry lecture an "interest interlude"—a few minutes of discussion about…”
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Interaction of sheep liver cytosolic aldehyde dehydrogenase with quercetin, resveratrol and diethylstilbestrol
Published in Chemico-biological interactions (30-01-2001)“…The effects of quercetin and resveratrol (substances found in red wine) on the activity of cytosolic aldehyde dehydrogenase in vitro are compared with those of…”
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Purple or Colorless - Which Way Up? An Entertaining Solubility Demonstration
Published in Journal of chemical education (01-08-2003)“…An entertaining demonstration is described that illustrates concepts of solubility and relies on the fact that solutions of potassium permanganate in water and…”
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Intermediates, Transition States, Butterflies, and Frogs
Published in Journal of chemical education (01-04-2001)“…An analogy that may be useful in the classroom is described. The changes that occur in typical simple SN1 and SN2 reactions are compared to the metamorphoses…”
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Comparison of Resorufin Acetate andp-Nitrophenyl Acetate as Substrates for Chymotrypsin
Published in Bioorganic chemistry (01-12-1996)“…Resorufin acetate is shown to be an attractive substrate to use with chymotrypsin since the absorbance of the product is several times more intense than that…”
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Studies on the chymotrypsin-catalysed hydrolysis of resorufin acetate and resorufin bromoacetate
Published in Biochimica et biophysica acta (11-06-1998)“…Resorufin bromoacetate is a substrate that is rapidly hydrolysed by chymotrypsin. The reaction shows a pre-steady-state burst phase that may be observed by…”
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Disulfide interchange reactions: An enzymic case study
Published in Journal of chemical education (01-09-1988)“…This article concerns the reaction known as "disulfide interchange" in which a thiol reacts with a disulfide to give a different thiol and disulfide pair as…”
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Effects of diethylstilbestrol, 2,2'-dithiodipyridine, and chloral hydrate on the esterase activity of sheep liver cytoplasmic aldehyde dehydrogenase
Published in Biochemistry (Easton) (12-08-1986)“…The binding of diethylstilbestrol (DES) to aldehyde dehydrogenase (ALDH) has a very similar effect on the dehydrogenase activity of the enzyme as has…”
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Sheep liver cytosolic aldehyde dehydrogenase: the structure reveals the basis for the retinal specificity of class 1 aldehyde dehydrogenases
Published in Structure (London) (15-12-1998)“…Background: Enzymes of the aldehyde dehydrogenase family are required for the clearance of potentially toxic aldehydes, and are essential for the production of…”
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Synthesis of methyl 2- and 4-pyridyl disulfide from 2- and 4-thiopyridone and methyl methanethiosulfonate
Published in Analytical biochemistry (01-05-1985)“…In 1982, methyl 2-pyridyl disulfide was reported as a new reagent for the titration of thiol groups in peptides and proteins and for their temporary blocking…”
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Studies with Potential Reporter Group Reagents for Enzymes: 3,4-Dihydro-3-(2-Hydroxyethyl)-6-Nitro-2H-1,3-Benzothiazin-2-Thione and 6-Nitrochromone—Hydrolysis and Interaction with Chymotrypsin
Published in Bioorganic chemistry (01-04-2000)“…3,4-Dihydro-3-(2-hydroxyethyl)-6-nitro-2H-1,3-benzothiazin-2-thione (II-HE), a cyclic dithiocarbamate, has the potential to react with an esterase to furnish…”
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The Oxidative Addition Reaction between Compounds of Resorufin (7-Hydroxy-3H-phenoxazin-3-one) and 2-Mercaptoethanol
Published in Bioorganic chemistry (01-08-1998)“…2-Mercaptoethanol reacts with the methanesulphonate, the dimethylcarbamate, and the ethyl ether of resorufin (7-hydroxy-3H-phenoxazin-3-one) at 25°C and pH 7.4…”
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Studies of the esterase activity of cytosolic aldehyde dehydrogenase with resorufin acetate as substrate
Published in Biochemical journal (15-03-1997)“…Resorufin acetate is a very good substrate for sheep liver cytosolic aldehyde dehydrogenase, both from the point of view of practical spectrophotometry and in…”
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3,4-Dihydro-3,4,6-trimethyl-2H,8H-pyrano-[3,2g]-1,3-benzoxazin-2,8-dione, a Potential Fluorogenic Reporter Group Reagent for Esterases—Synthesis and Interaction with Chymotrypsin
Published in Bioorganic chemistry (01-10-2000)“…The synthesis of a novel “reporter group” reagent—3,4-dihydro-3,4,6-trimethyl-2H,8H-pyrano-[3,2g]-1,3-benzoxazin-2,8-dione (DTPBD)—is described. This compound…”
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A comparison of nitrophenyl esters and lactones as substrates of cytosolic aldehyde dehydrogenase
Published in Biochemical journal (15-05-1996)“…1. p-Nitrophenyl (PNP) acetate and propionate show a burst of p-nitrophenoxide release when their hydrolysis is catalysed by sheep liver cytosolic aldehyde…”
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The action of cytosolic aldehyde dehydrogenase on resorufin acetate
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The effect of quercetin, a widely distributed flavonoid in food and drink, on cytosolic aldehyde dehydrogenase: a comparison with the effect of diethylstilboestrol
Published in Biochimica et biophysica acta (29-09-2000)“…Quercetin is a flavonoid found in red wine and many other dietary sources. Observations concerning the state of ionisation and the stability of the compound…”
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Covalent Modification Reactions Involving Cytosolic Aldehyde Dehydrogenase and a Variety of Resorufin Derivatives
Published in Bioorganic chemistry (01-08-1998)“…The bromoacetates of resorufin and ofp-nitrophenol act simultaneously as very rapidly hydrolyzed substrates and as potent irreversible inactivators of…”
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