Rice RCN1/OsABCG5 mutation alters accumulation of essential and nonessential minerals and causes a high Na/K ratio, resulting in a salt-sensitive phenotype

Mineral balance and salt stress are major factors affecting plant growth and yield. Here, we characterized the effects of rice (Oryza sativa L.) reduced culm number1 (rcn1), encoding a G subfamily ABC transporter (OsABCG5) involved in accumulation of essential and nonessential minerals, the Na/K rat...

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Published in:	Plant science (Limerick) Vol. 224; pp. 103 - 111
Main Authors:	Matsuda, Shuichi, Nagasawa, Hidetaka, Yamashiro, Nobuhiro, Yasuno, Naoko, Watanabe, Toshihiro, Kitazawa, Hideyuki, Takano, Sho, Tokuji, Yoshihiko, Tani, Masayuki, Takamure, Itsuro, Kato, Kiyoaki
Format:	Journal Article
Language:	English
Published:	Ireland 01-07-2014
Subjects:	Adaptation, Physiological - genetics ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - metabolism Cation Transport Proteins Gene Expression Gene Expression Regulation, Plant Genes, Plant Homeostasis Minerals - metabolism Mutation Oryza - genetics Oryza - metabolism Oryza sativa Phenotype Plant Proteins - genetics Plant Proteins - metabolism Plant Roots - metabolism Plant Shoots - metabolism Potassium - metabolism Sodium - metabolism Sodium Chloride - metabolism Stress, Physiological - genetics Symporters Salt tolerance Na/K ratio Mineral balance ABC transporter SKC1/OsHKT1 ;5
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Summary:	Mineral balance and salt stress are major factors affecting plant growth and yield. Here, we characterized the effects of rice (Oryza sativa L.) reduced culm number1 (rcn1), encoding a G subfamily ABC transporter (OsABCG5) involved in accumulation of essential and nonessential minerals, the Na/K ratio, and salt tolerance. Reduced potassium and elevated sodium in field-grown plants were evident in rcn1 compared to original line 'Shiokari' and four independent rcn mutants, rcn2, rcn4, rcn5 and rcn6. A high Na/K ratio was evident in the shoots and roots of rcn1 under K starvation and salt stress in hydroponically cultured plants. Downregulation of SKC1/OsHKT1;5 in rcn1 shoots under salt stress demonstrated that normal function of RCN1/OsABCG5 is essential for upregulation of SKC1/OsHKT1;5 under salt stress. The accumulation of various minerals in shoots and roots was also altered in the rcn1 mutant compared to 'Shiokari' under control conditions, potassium starvation, and salt and d-sorbitol treatments. The rcn1 mutation resulted in a salt-sensitive phenotype. We concluded that RCN1/OsABCG5 is a salt tolerance factor that acts via Na/K homeostasis, at least partly by regulation of SKC1/OsHKT1;5 in shoots.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0168-9452 1873-2259
DOI:	10.1016/j.plantsci.2014.04.011