An Outbreak of Group C Rotavirus Gastroenteritis among Adults Living in Valentim Gentil, São Paulo State, Brazil

An Outbreak of Group C Rotavirus Gastroenteritis among Adults Living in Valentim Gentil, São Paulo State, Brazil

An outbreak of gastroenteritis affecting adults and children occurred in the small city of Valentim Gentil, São Paulo, Brazil, in 1993. Nineteen faecal samples (from 10 cases and 9 contacts) were examined by direct electron microscopy (DEM), immune electron microscopy (IEM), polyacrylamide gel elect...

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Bibliographic Details
Published in:Journal of diarrhoeal diseases research Vol. 16; no. 2; pp. 59 - 65
Main Authors: Souza, Denise FC, Kisielius, Jonas J, Ueda, Marli, Gabbay, Yvone B, Carmona, Rita CC, Timenetsky, Maria do Carmo ST, Mascarenhas, Joana DP, Takimoto, Sueko, Tanaka, Hatune
Format: Journal Article
Language:English
Published: Bangladesh International Centre for Diarrhoeal Disease Research 01-06-1998
Subjects:
Adolescent
Adult
Adults
Brazil - epidemiology
Child
Child, Preschool
Children
Diarrhea
Disease Outbreaks
Electron microscopy
Enzyme linked immunosorbent assay
Epidemics
Female
Gastroenteritis
Gastroenteritis - epidemiology
Humans
Infant
Male
Middle Aged
Rotavirus
Viruses
Brazil
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Summary:An outbreak of gastroenteritis affecting adults and children occurred in the small city of Valentim Gentil, São Paulo, Brazil, in 1993. Nineteen faecal samples (from 10 cases and 9 contacts) were examined by direct electron microscopy (DEM), immune electron microscopy (IEM), polyacrylamide gel electrophoresis (PAGE), and enzyme-linked immunosorbent assay (ELISA) for group A and C rotaviruses. DEM detected rotavirus in 6 of the 10 cases and in none of the contacts. All of the samples were negative for group A rotavirus by ELISA. Analysis by PAGE showed an electrophoretic profile suggestive of group C rotavirus in two cases. Group C rotavirus was identified by IEM in 4 of the cases and in 1 of the contacts. All of the samples were submitted to ELISA for group C rotavirus. This resulted in a total of 10 positives — 7 for diarrhoeal cases and 3 for contacts. This outbreak was strongly associated with group C rotavirus. The importance of combining different diagnostic methods is emphasised.
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ISSN:0253-8768
2311-8512