Uptake of Indium-111 in the Liver of Mice Following Administration of Indium-111-DTPA-Labeled Monoclonal Antibodies: Influence of Labeling Parameters, Physiologic Parameters, and Antibody Dose

Liver uptake of indium-111 (111In) in mice was investigated following administration of 111In-DTPA murine monoclonal antibodies (111In-DTPA-MAbs) labeled by the cyclic anhydride method. Biodistribution of HPLC-purified 111In-DTPA-MAb preparations was checked with a low (0.2 micrograms) and a high (8...

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Bibliographic Details
Published in:	The Journal of nuclear medicine (1978) Vol. 31; no. 6; pp. 1084 - 1093
Main Authors:	Schuhmacher, Jochen, Klivenyi, Gabor, Matys, Ronald, Kirchgebner, Henning, Hauser, Harald, Maier-Borst, Wolfgang, Matzku, Siegfried
Format:	Journal Article
Language:	English
Published:	Reston, VA Soc Nuclear Med 01-06-1990 Society of Nuclear Medicine
Subjects:	550601 - Medicine- Unsealed Radionuclides in Diagnostics 550604 - Medicine- Unsealed Radionuclides in Therapy- (1980-) AMINO ACIDS ANIMALS ANTIBODIES Antibodies, Monoclonal - administration & dosage BETA DECAY RADIOISOTOPES Biological and medical sciences BIOLOGICAL VARIABILITY BODY CARBOXYLIC ACIDS CHELATING AGENTS Contrast media. Radiopharmaceuticals DAYS LIVING RADIOISOTOPES DIGESTIVE SYSTEM DISTRIBUTION DOSES DRUGS DTPA ELECTRON CAPTURE RADIOISOTOPES EXPERIMENTAL NEOPLASMS GENETIC VARIABILITY GLANDS INDIUM 111 INDIUM ISOTOPES Indium Radioisotopes - administration & dosage Indium Radioisotopes - pharmacokinetics INTERMEDIATE MASS NUCLEI ISOMERIC TRANSITION ISOTOPES Isotope Labeling ISOTOPES LABELLING LIVER Liver - metabolism MAMMALS Medical sciences MICE MINUTES LIVING RADIOISOTOPES MONOCLONAL ANTIBODIES NUCLEI ODD-EVEN NUCLEI ORGANIC ACIDS ORGANIC COMPOUNDS ORGANS Pentetic Acid - administration & dosage Pharmacology. Drug treatments RADIATION DOSES RADIOISOTOPES RADIOLOGY AND NUCLEAR MEDICINE RADIOPROTECTIVE SUBSTANCES RODENTS TISSUE DISTRIBUTION VERTEBRATES Radiolabelling Animal Liver Monoclonal antibody Review Radiopharmaceuticals Uptake
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Summary:	Liver uptake of indium-111 (111In) in mice was investigated following administration of 111In-DTPA murine monoclonal antibodies (111In-DTPA-MAbs) labeled by the cyclic anhydride method. Biodistribution of HPLC-purified 111In-DTPA-MAb preparations was checked with a low (0.2 micrograms) and a high (8.0 micrograms) MAb dose. Using Bio Gel P-30 for desalting the MAb-conjugates, 111In uptake in the liver amounted to 8%-9% of the injected dose (ID) and was independent from the MAb dose, the DTPA-to-MAb molar ratio, tumor growth and biologic variability (different MAbs and different strains of mice). Using Sephadex G-25 for desalting, 0.2 micrograms doses from 7 out of 26 preparations showed increased liver accumulation of 111In in non-tumor mice ranging from 15%-25% of ID. Corresponding high doses led to a "normal" value of 8%-9%. Increased liver uptake of the low dose could not be reduced by coadministration of the unconjugated MAb, but was normal after reinjection of "in vivo filtered" material. An inverse intracellular distribution of 111In activity between sediment and supernatant of liver homogenates, following the administration of the low and the high MAb dose, indicated an artifact of the labeling procedure rather than an inherent biological property of labeled MAbs.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0161-5505 1535-5667