Search Results - "Kinoshita, Emiko"

Improved Phos-tag SDS-PAGE under neutral pH conditions for advanced protein phosphorylation profiling by Kinoshita, Eiji, Kinoshita-Kikuta, Emiko

“…We describe an improved Phos-tag SDS-PAGE (Zn²⁺-Phos-tag SDS-PAGE) using a dizinc(II) complex of Phos-tag acrylamide in conjunction with a Bis-tris-buffered…”
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Anti-Influenza Virus Effects of Elderberry Juice and Its Fractions by KINOSHITA, Emiko, HAYASHI, Kyoko, KATAYAMA, Hiroshi, HAYASHI, Toshimitsu, OBATA, Akio

“…Elderberry (Sambucus nigra L.) has traditionally been used for treating influenza and colds. We evaluated the antiviral effect of concentrated juice of…”
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Evaluation of four phosphopeptide enrichment strategies for mass spectrometry‐based proteomic analysis by Ino, Yoko, Kinoshita, Eiji, Kinoshita‐Kikuta, Emiko, Akiyama, Tomoko, Nakai, Yusuke, Nishino, Kohei, Osada, Makoto, Ryo, Akihide, Hirano, Hisashi, Koike, Tohru, Kimura, Yayoi

“…Information about phosphorylation status can be used to prioritize and characterize biological processes in the cell. Various analytical strategies have been…”
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Inhibition of Glucose Transport by Tomatoside A, a Tomato Seed Steroidal Saponin, through the Suppression of GLUT2 Expression in Caco‑2 Cells by Li, Baorui, Terazono, Yusuke, Hirasaki, Naoto, Tatemichi, Yuki, Kinoshita, Emiko, Obata, Akio, Matsui, Toshiro

“…We investigated whether tomatoside A (5α-furostane-3β,22,26-triol-3-[O-β-d-glucopyranosyl (1→2)-β-d-glucopyranosyl (1→4)-β-d-galactopyranoside]…”
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Label-free Kinase Profiling Using Phosphate Affinity Polyacrylamide Gel Electrophoresis by Kinoshita-Kikuta, Emiko, Aoki, Yuri, Kinoshita, Eiji, Koike, Tohru

“…Herein we describe three applications of label-free kinase profiling using a novel type of phosphate affinity polyacrylamide gel electrophoresis. The phosphate…”
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Characterization of zinc(II) complex of 1,4,7,10-tetrazacyclododecane and deprotonated 5-fluorouracil (FU) in crystalline/solution states and evaluation of anticancer activity: Approach for improving the anticancer activity of FU by Ichimaru, Yoshimi, Kato, Koichi, Nakatani, Rina, Sugiura, Kirara, Mizutani, Hideki, Kinoshita-Kikuta, Emiko, Koike, Tohru, Jin, Wanchun, Imai, Masanori, Kurosaki, Hiromasa

“…[Display omitted] •FU–-bound Zn(II)–cyclen complex was characterized in the crystalline/solution states.•X-ray crystallography revealed the complex structure…”
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Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during intracellular protein traffic by Kinoshita-Kikuta, Emiko, Utsumi, Toshihiko, Miyazaki, Aya, Tokumoto, Chiharu, Doi, Kyosuke, Harada, Haruna, Kinoshita, Eiji, Koike, Tohru

“…Protein N-myristoylation of Src-family kinases (SFKs) is a critical co-translational modification to anchor the enzymes in the plasma membrane. Phosphorylation…”
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Tips on improving the efficiency of electrotransfer of target proteins from Phos-tag SDS-PAGE gel by Kinoshita-Kikuta, Emiko, Kinoshita, Eiji, Matsuda, Ayumi, Koike, Tohru

“…The sensitivity of Western blotting analysis after Phos‐tag SDS‐PAGE is occasionally inferior to that after normal (Phos‐tag‐free) SDS‐PAGE under similar…”
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Quantitative monitoring of His and Asp phosphorylation in a bacterial signaling system by using Phos‐tag Magenta/Cyan fluorescent dyes by Kinoshita‐Kikuta, Emiko, Kusamoto, Hiroshi, Ono, Syogo, Akayama, Keisuke, Eguchi, Yoko, Igarashi, Masayuki, Okajima, Toshihide, Utsumi, Ryutaro, Kinoshita, Eiji, Koike, Tohru

“…In the bacterial signaling mechanisms known as two‐component systems (TCSs), signals are generally conveyed by means of a His–Asp phosphorelay. Each system…”
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Separation and detection of large phosphoproteins using Phos-tag SDS-PAGE by Kinoshita, Eiji, Kinoshita-Kikuta, Emiko, Koike, Tohru

“…We provide a standard phosphate-affinity SDS-PAGE (Mn 2+ –Phos-tag SDS-PAGE) protocol, in which Phos-tag is used to analyze large phosphoproteins with…”
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A Phos‐tag‐based micropipette‐tip method for rapid and selective enrichment of phosphopeptides by Yuan, Elena Tianfei, Ino, Yoko, Kawaguchi, Maho, Kimura, Yayoi, Hirano, Hisashi, Kinoshita‐Kikuta, Emiko, Kinoshita, Eiji, Koike, Tohru

“…Phosphorylated peptides are attractive targets in the study of the phosphoproteome. Here, we introduce a simple and convenient micropipette‐tip method for the…”
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History of Phos-tag technology for phosphoproteomics by Kinoshita, Eiji, Kinoshita-Kikuta, Emiko, Koike, Tohru

“…Phos-tag is a functional molecule that selectively captures a phosphate monoester dianion in neutral aqueous solutions. The affinity of Phos-tag for phosphate…”
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Separation and identification of four distinct serine-phosphorylation states of ovalbumin by Phos-tag affinity electrophoresis by Kinoshita-Kikuta, Emiko, Kinoshita, Eiji, Koike, Tohru

“…Ovalbumin (OVA) derived from egg white contains two residues that can be phosphorylated: Ser‐68 and Ser‐344. Native polyacrylamide gel electrophoresis(PAGE)…”
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Antihypertensive Effect of Peptide-Enriched Soy Sauce-Like Seasoning and Identification of Its Angiotensin I-Converting Enzyme Inhibitory Substances by Nakahara, Takeharu, Sano, Atsushi, Yamaguchi, Hitomi, Sugimoto, Katsutoshi, Chikata, Hiroyuki, Kinoshita, Emiko, Uchida, Riichiro

“…We have developed a peptide-enriched soy sauce-like seasoning termed Fermented Soybean Seasoning (FSS), by modifying the process of soy sauce brewing. The FSS…”
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Specific glutamic acid residues in targeted proteins induce exaggerated retardations in Phos‐tag SDS‐PAGE migration by Kinoshita, Eiji, Kinoshita‐Kikuta, Emiko, Karata, Kiyonobu, Kawano, Toshiki, Nishiyama, Atsuhiro, Yamato, Morihisa, Koike, Tohru

“…We describe two unique proteins, Escherichia coli ClpX and human histone H2A, that show extremely retarded migrations relative to their molecular weights in…”
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Highly sensitive detection of protein phosphorylation by using improved Phos-tag Biotin by Kinoshita, Eiji, Kinoshita-Kikuta, Emiko, Sugiyama, Yasunori, Fukada, Yoshitaka, Ozeki, Tomomitsu, Koike, Tohru

“…We have previously shown that the dinuclear zinc(II) complex Phos‐tag and its derivatives act as phosphate‐capture molecules in aqueous solution under…”
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Phosphate-binding Tag, a New Tool to Visualize Phosphorylated Proteins by Kinoshita, Eiji, Kinoshita-Kikuta, Emiko, Takiyama, Kei, Koike, Tohru

“…We introduce two methods for the visualization of phosphorylated proteins using alkoxide-bridged dinuclear metal (i.e. Zn2+ or Mn2+) complexes as novel…”
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Advances in Phos-tag-based methodologies for separation and detection of the phosphoproteome by Kinoshita, Eiji, Kinoshita-Kikuta, Emiko, Koike, Tohru

“…This review article describes analytical techniques based on the phosphate-binding tag molecule “Phos-tag”, which is an alkoxide-bridged dinuclear metal…”
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Profiling of protein thiophosphorylation by Phos-tag affinity electrophoresis: Evaluation of adenosine 5′-O-(3-thiotriphosphate) as a phosphoryl donor in protein kinase reactions by Kinoshita, Eiji, Kinoshita-Kikuta, Emiko, Shiba, Akio, Edahiro, Keisuke, Inoue, Yuki, Yamamoto, Kaneyoshi, Yoshida, Myu, Koike, Tohru

“…Adenosine 5′‐O‐(3‐thiotriphosphate) (ATPγS) has been widely used as a phosphoryl donor to trace protein kinase activities. However, the question remains…”
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Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE by Kinoshita, Eiji, Kinoshita-Kikuta, Emiko, Matsubara, Mamoru, Yamada, Seiji, Nakamura, Hiro, Shiro, Yoshitsugu, Aoki, Yuri, Okita, Kouki, Koike, Tohru

“…Herein, we demonstrate the separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE. The…”
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