Search Results - "Kingsman, A.J"

Symmetry, flexibility and permeability in the structure of yeast retrotransposon virus-like particles by Burns, N.R, Saibil, H.R, White, N.S, Pardon, J.F, Timmins, P.A, Richardson, S.M.H, Richards, B.M, Adams, S.E, Kingsman, S.M, Kingsman, A.J

“…The virus-like particles (VLPs) of the yeast retrotransposon Ty are genetically, structurally and functionally analogous to retroviral nucleocapsids or cores…”
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Factors affecting heterologous gene expression in Saccharomyces cerevisiae by Mellor, J, Dobson, M J, Roberts, N A, Kingsman, A J, Kingsman, S M

“…The 'promoter' fragment from the yeast phosphoglycerate kinase (PGK) gene has been used to direct the expression of human interferon-alpha-2 (IFN alpha 2) on a…”
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Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae by Mellor, J, Dobson, M J, Roberts, N A, Tuite, M F, Emtage, J S, White, S, Lowe, P A, Patel, T, Kingsman, A J, Kingsman, S M

“…We have constructed a high-efficiency expression vector to direct the synthesis of heterologous polypeptides in yeast. The vector is termed a sandwich…”
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Polyvalent recombinant antigens: a new vaccine strategy by Kingsman, S M, Kingsman, A J

“…Recombinant DNA technology can be used to produce individual proteins from infectious organisms and these have the potential to be used as subunit vaccines. In…”
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Production and purification of hybrid ty-VLPs by Burns, N.R. (British Bio-Technology Ltd., Oxford, UK.), Gilmour, J.E.M, Kingsman, S.M, Kingsman, A.J, Adams, S.E

“…This article describes how pure Ty-VLPs (virus-like particles) can be prepared from hybrid Ty-VLPs. Many different hybrid Ty-VLPs have been produced and may be…”
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Regulated high efficiency expression of human interferon‐alpha in Saccharomyces cerevisiae by Tuite, M.F., Dobson, M.J., Roberts, N.A., King, R.M., Burke, D.C., Kingsman, S.M., Kingsman, A.J.

“…The 5′ control region of the yeast phosphoglycerate kinase gene (PGK) was fused to the coding sequence of a human interferon‐alpha. This PGK‐interferon fusion…”
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Expression vectors for the construction of hybrid ty-VLPs by ADAMS, S. E, RICHARDSON, S. M. H, KINGSMAN, S. M, KINGSMAN, A. J

“…Purification of expressed proteins can be facilitated by expressing the recombinant protein as a fusion with a carrier protein that assembles into particulate…”
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Sequence variation in the LEU2 region of the saccharomyces cerevisiae genome by Dobson, M J, Kingsman, S M, Kingsman, A J

“…The LEU2 regions present on yeast plasmid vectors come from two sources, a series of strains derived from S288c and strain M127. The LEU2 region from the S288c…”
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A Physiologically Regulated Adenoviral Vector for the Treatment of Ischaemic Disease by Kingsman, S.M., Binley, K., Griffiths, L., Iqball, S., Spearman, H., Kingsman, A.J., Naylor, S.

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Preclinical Evaluation of Human Cytochrome P450 2b6 (Cyp2b6) as a Therapeutic Gene Delivered by a Retroviral Vehicle for Gene-Directed Enzyme Prodrug Therapy (GDEPT) of Cancer by Kingsman, S.M., Kan, O., Griffiths, L., Iqball, S., Baban, D., Uden, M., Spearman, H., Slade, A., Kingsman, A.J., Naylor, S.

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Cryo-electron microscopy structure of yeast Ty retrotransposon virus-like particles by Palmer, K.J. (Birkbeck College, London.), Tichelaar, W, Myers, N, Burns, N.R, Butcher, S.J, Kingsman, A.J, Fuller, S.D, Saibil, H.R

“…The virus-like particles (VLPs) produced by the yeast retrotransposon Ty1 are functionally related to retroviral cores. These particles are unusual in that…”
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Promoter control of translation in Xenopus oocytes by Gunkel, N., Braddock, M., Thorburn, A.M., Muckenthaler, M., Kingsman, A.J., Kingsman, S.M.

“…The HIV-1 promoter directs the high level production of transcripts in Xenopus oocytes. However, despite being exported to the cytoplasm, the transcripts are…”
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Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae by Dobson, M.J., Tuite, M.F., Roberts, N.A., Kingsman, A.J., Kingsman, S.M., Perkins, R.E., Conroy, S.C., Dunbar, B., Fothergill, L.A.

“…The position of the yeast phosphoglycerate kinase (PGK) gene has been mapped on a 2.95kb Hind III fragment. We have determined the nucleotide sequence of the…”
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Identification of proteolytic cleavage sites within the gag-analogue protein of Ty1 virus-like particles by Martin-Rendon, E, Hurd, D W, Marfany, G, Kingsman, S M, Kingsman, A J

“…Like retroviruses, the yeast retrotransposon Ty1 produces its proteins as precursors that are subsequently cleaved by a protease encoded by the element. These…”
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High-efficiency yeast expression vectors based on the promoter of the phosphoglycerate kinase gene by Kingsman, S M, Cousens, D, Stanway, C A, Chambers, A, Wilson, M, Kingsman, A J

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The effects of adjuvants on CTL induction by V3:Ty-virus-like particles (V3-VLPs) in mice by Harris, S.J., Woodrow, S.A., Gearing, A.J.H., Adams, S.E., Kingsman, A.J., Layton, G.T.

“…We have previously described the generation of HIV-1 V3-specific cytotoxic T-lymphocytes (CTL) responses in BALB c (H-2 d) mice following immunization with…”
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Phosphorylation influences the binding of the yeast RAP1 protein to the upstream activating sequence of the PGK gene by Tsang, J.S.H, Henry, Y.A.L, Chambers, A, Kingsman, A.J, Kingsman, S.M

“…Yeast repressor activator protein 1 (RAP1) binds in vitro to specific DNA sequences that are found in diverse genetic elements. Expression of the yeast…”
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Multiple cooperative interactions constrain BPV-1 E2 dependent activation of transcription by SOWDEN, M, HARRISON, S, ASHFIELD, R, KINGSMAN, A. J, KINGSMAN, S. M

“…Transcription directed by the BPV-1 long control region (LCR) is conditional upon activation by the virally encoded E2 protein. Within the 1.0 kb LCR there are…”
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Characterisation of the DNA binding domain of the yeast RAP1 protein by Henry, Y.L., Chambers, A., Tsang, J.S.H., Kingsman, A.J., Kingsman, S.M.

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Retroelement particles as purification, presentation and targeting vehicles by Kingsman, A J, Adams, S E, Burns, N R, Kingsman, S M

“…The manipulation of retrotransposon and retroviral particles to carry biologically active molecules is becoming feasible. In addition, recent experiments…”
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