Search Results - "King, Neil P"

Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding by Starr, Tyler N., Greaney, Allison J., Hilton, Sarah K., Ellis, Daniel, Crawford, Katharine H.D., Dingens, Adam S., Navarro, Mary Jane, Bowen, John E., Tortorici, M. Alejandra, Walls, Alexandra C., King, Neil P., Veesler, David, Bloom, Jesse D.

“…The receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein mediates viral attachment to ACE2 receptor and is a major determinant of host range and…”
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Accurate design of megadalton-scale two-component icosahedral protein complexes by Bale, Jacob B., Gonen, Shane, Liu, Yuxi, Sheffler, William, Ellis, Daniel, Thomas, Chantz, Cascio, Duilio, Yeates, Todd O., Gonen, Tamir, King, Neil P., Baker, David

“…Nature provides many examples of self- and co-assembling protein-based molecular machines, including icosahedral protein cages that serve as scaffolds,…”
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Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19 by Rodda, Lauren B., Netland, Jason, Shehata, Laila, Pruner, Kurt B., Morawski, Peter A., Thouvenel, Christopher D., Takehara, Kennidy K., Eggenberger, Julie, Hemann, Emily A., Waterman, Hayley R., Fahning, Mitchell L., Chen, Yu, Hale, Malika, Rathe, Jennifer, Stokes, Caleb, Wrenn, Samuel, Fiala, Brooke, Carter, Lauren, Hamerman, Jessica A., King, Neil P., Gale, Michael, Campbell, Daniel J., Rawlings, David J., Pepper, Marion

“…The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus is causing a global pandemic, and cases continue to rise. Most infected individuals…”
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Dynamics of Neutralizing Antibody Titers in the Months After Severe Acute Respiratory Syndrome Coronavirus 2 Infection by Crawford, Katharine H D, Dingens, Adam S, Eguia, Rachel, Wolf, Caitlin R, Wilcox, Naomi, Logue, Jennifer K, Shuey, Kiel, Casto, Amanda M, Fiala, Brooke, Wrenn, Samuel, Pettie, Deleah, King, Neil P, Greninger, Alexander L, Chu, Helen Y, Bloom, Jesse D

“…Abstract Most individuals infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) develop neutralizing antibodies that target the viral…”
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Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays by Crawford, Katharine H D, Eguia, Rachel, Dingens, Adam S, Loes, Andrea N, Malone, Keara D, Wolf, Caitlin R, Chu, Helen Y, Tortorici, M Alejandra, Veesler, David, Murphy, Michael, Pettie, Deleah, King, Neil P, Balazs, Alejandro B, Bloom, Jesse D

“…SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera…”
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Hallmarks of icosahedral virus capsids emerged during laboratory evolution of a bacterial enzyme by Olshefsky, Audrey, King, Neil P.

“…Viruses are fascinating molecular machines that inspire many therapeutic design efforts. Tetter, Terasaka, Steinauer et al. recently reported the laboratory…”
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Induction of Potent Neutralizing Antibody Responses by a Designed Protein Nanoparticle Vaccine for Respiratory Syncytial Virus by Marcandalli, Jessica, Fiala, Brooke, Ols, Sebastian, Perotti, Michela, de van der Schueren, Willem, Snijder, Joost, Hodge, Edgar, Benhaim, Mark, Ravichandran, Rashmi, Carter, Lauren, Sheffler, Will, Brunner, Livia, Lawrenz, Maria, Dubois, Patrice, Lanzavecchia, Antonio, Sallusto, Federica, Lee, Kelly K., Veesler, David, Correnti, Colin E., Stewart, Lance J., Baker, David, Loré, Karin, Perez, Laurent, King, Neil P.

“…Respiratory syncytial virus (RSV) is a worldwide public health concern for which no vaccine is available. Elucidation of the prefusion structure of the RSV F…”
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Designed proteins induce the formation of nanocage-containing extracellular vesicles by Votteler, Jörg, Ogohara, Cassandra, Yi, Sue, Hsia, Yang, Nattermann, Una, Belnap, David M., King, Neil P., Sundquist, Wesley I.

“…Autonomously produced hybrid biological nanomaterials termed ‘enveloped protein nanocages’ incorporate features for membrane binding, self-assembly, and ESCRT…”
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Complete and cooperative in vitro assembly of computationally designed self-assembling protein nanomaterials by Wargacki, Adam J., Wörner, Tobias P., van de Waterbeemd, Michiel, Ellis, Daniel, Heck, Albert J. R., King, Neil P.

“…Recent advances in computational methods have enabled the predictive design of self-assembling protein nanomaterials with atomic-level accuracy. These design…”
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Practical approaches to designing novel protein assemblies by King, Neil P, Lai, Yen-Ting

“…•Recent studies focusing on designing highly ordered protein assemblies are reviewed.•Principles related to the spatial organization of subunits are…”
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Enhancing and shaping the immunogenicity of native-like HIV-1 envelope trimers with a two-component protein nanoparticle by Brouwer, Philip J. M., Antanasijevic, Aleksandar, Berndsen, Zachary, Yasmeen, Anila, Fiala, Brooke, Bijl, Tom P. L., Bontjer, Ilja, Bale, Jacob B., Sheffler, William, Allen, Joel D., Schorcht, Anna, Burger, Judith A., Camacho, Miguel, Ellis, Daniel, Cottrell, Christopher A., Behrens, Anna-Janina, Catalano, Marco, del Moral-Sánchez, Iván, Ketas, Thomas J., LaBranche, Celia, van Gils, Marit J., Sliepen, Kwinten, Stewart, Lance J., Crispin, Max, Montefiori, David C., Baker, David, Moore, John P., Klasse, Per Johan, Ward, Andrew B., King, Neil P., Sanders, Rogier W.

“…The development of native-like HIV-1 envelope (Env) trimer antigens has enabled the induction of neutralizing antibody (NAb) responses against…”
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De novo design of tunable, pH-driven conformational changes by Boyken, Scott E, Benhaim, Mark A, Busch, Florian, Jia, Mengxuan, Bick, Matthew J, Choi, Heejun, Klima, Jason C, Chen, Zibo, Walkey, Carl, Mileant, Alexander, Sahasrabuddhe, Aniruddha, Wei, Kathy Y, Hodge, Edgar A, Byron, Sarah, Quijano-Rubio, Alfredo, Sankaran, Banumathi, King, Neil P, Lippincott-Schwartz, Jennifer, Wysocki, Vicki H, Lee, Kelly K, Baker, David

“…The ability of naturally occurring proteins to change conformation in response to environmental changes is critical to biological function. Although there have…”
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Tailored design of protein nanoparticle scaffolds for multivalent presentation of viral glycoprotein antigens by Ueda, George, Antanasijevic, Aleksandar, Fallas, Jorge A, Sheffler, William, Copps, Jeffrey, Ellis, Daniel, Hutchinson, Geoffrey B, Moyer, Adam, Yasmeen, Anila, Tsybovsky, Yaroslav, Park, Young-Jun, Bick, Matthew J, Sankaran, Banumathi, Gillespie, Rebecca A, Brouwer, Philip Jm, Zwart, Peter H, Veesler, David, Kanekiyo, Masaru, Graham, Barney S, Sanders, Rogier W, Moore, John P, Klasse, Per Johan, Ward, Andrew B, King, Neil P, Baker, David

“…Multivalent presentation of viral glycoproteins can substantially increase the elicitation of antigen-specific antibodies. To enable a new generation of…”
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Serological identification of SARS-CoV-2 infections among children visiting a hospital during the initial Seattle outbreak by Dingens, Adam S., Crawford, Katharine H. D., Adler, Amanda, Steele, Sarah L., Lacombe, Kirsten, Eguia, Rachel, Amanat, Fatima, Walls, Alexandra C., Wolf, Caitlin R., Murphy, Michael, Pettie, Deleah, Carter, Lauren, Qin, Xuan, King, Neil P., Veesler, David, Krammer, Florian, Dickerson, Jane A., Chu, Helen Y., Englund, Janet A., Bloom, Jesse D.

“…Children are strikingly underrepresented in COVID-19 case counts. In the United States, children represent 22% of the population but only 1.7% of confirmed…”
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A broadly generalizable stabilization strategy for sarbecovirus fusion machinery vaccines by Lee, Jimin, Stewart, Cameron, Schäfer, Alexandra, Leaf, Elizabeth M., Park, Young-Jun, Asarnow, Daniel, Powers, John M., Treichel, Catherine, Sprouse, Kaitlin R., Corti, Davide, Baric, Ralph, King, Neil P., Veesler, David

“…Evolution of SARS-CoV-2 alters the antigenicity of the immunodominant spike (S) receptor-binding domain and N-terminal domain, undermining the efficacy of…”
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Structural and functional evaluation of de novo-designed, two-component nanoparticle carriers for HIV Env trimer immunogens by Antanasijevic, Aleksandar, Ueda, George, Brouwer, Philip J M, Copps, Jeffrey, Huang, Deli, Allen, Joel D, Cottrell, Christopher A, Yasmeen, Anila, Sewall, Leigh M, Bontjer, Ilja, Ketas, Thomas J, Turner, Hannah L, Berndsen, Zachary T, Montefiori, David C, Klasse, Per Johan, Crispin, Max, Nemazee, David, Moore, John P, Sanders, Rogier W, King, Neil P, Baker, David, Ward, Andrew B

“…Two-component, self-assembling nanoparticles represent a versatile platform for multivalent presentation of viral antigens. Computational design of protein…”
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Induction of cross-neutralizing antibodies by a permuted hepatitis C virus glycoprotein nanoparticle vaccine candidate by Sliepen, Kwinten, Radić, Laura, Capella-Pujol, Joan, Watanabe, Yasunori, Zon, Ian, Chumbe, Ana, Lee, Wen-Hsin, de Gast, Marlon, Koopsen, Jelle, Koekkoek, Sylvie, del Moral-Sánchez, Iván, Brouwer, Philip J. M., Ravichandran, Rashmi, Ozorowski, Gabriel, King, Neil P., Ward, Andrew B., van Gils, Marit J., Crispin, Max, Schinkel, Janke, Sanders, Rogier W.

“…Hepatitis C virus (HCV) infection affects approximately 58 million people and causes ~300,000 deaths yearly. The only target for HCV neutralizing antibodies is…”
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Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM by Antanasijevic, Aleksandar, Sewall, Leigh M., Cottrell, Christopher A., Carnathan, Diane G., Jimenez, Luis E., Ngo, Julia T., Silverman, Jennifer B., Groschel, Bettina, Georgeson, Erik, Bhiman, Jinal, Bastidas, Raiza, LaBranche, Celia, Allen, Joel D., Copps, Jeffrey, Perrett, Hailee R., Rantalainen, Kimmo, Cannac, Fabien, Yang, Yuhe R., de la Peña, Alba Torrents, Rocha, Rebeca Froes, Berndsen, Zachary T., Baker, David, King, Neil P., Sanders, Rogier W., Moore, John P., Crotty, Shane, Crispin, Max, Montefiori, David C., Burton, Dennis R., Schief, William R., Silvestri, Guido, Ward, Andrew B.

“…Engineered ectodomain trimer immunogens based on BG505 envelope glycoprotein are widely utilized as components of HIV vaccine development platforms. In this…”
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Structure-based design of stabilized recombinant influenza neuraminidase tetramers by Ellis, Daniel, Lederhofer, Julia, Acton, Oliver J., Tsybovsky, Yaroslav, Kephart, Sally, Yap, Christina, Gillespie, Rebecca A., Creanga, Adrian, Olshefsky, Audrey, Stephens, Tyler, Pettie, Deleah, Murphy, Michael, Sydeman, Claire, Ahlrichs, Maggie, Chan, Sidney, Borst, Andrew J., Park, Young-Jun, Lee, Kelly K., Graham, Barney S., Veesler, David, King, Neil P., Kanekiyo, Masaru

“…Influenza virus neuraminidase (NA) is a major antiviral drug target and has recently reemerged as a key target of antibody-mediated protective immunity. Here…”
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Fast and versatile sequence-independent protein docking for nanomaterials design using RPXDock by Sheffler, William, Yang, Erin C, Dowling, Quinton, Hsia, Yang, Fries, Chelsea N, Stanislaw, Jenna, Langowski, Mark D, Brandys, Marisa, Li, Zhe, Skotheim, Rebecca, Borst, Andrew J, Khmelinskaia, Alena, King, Neil P, Baker, David

“…Computationally designed multi-subunit assemblies have shown considerable promise for a variety of applications, including a new generation of potent vaccines…”
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