The comprehensive in vitro evaluation of eight different calcium phosphates: Significant parameters for cell behavior

The comprehensive in vitro evaluation of eight different calcium phosphates: Significant parameters for cell behavior

Eight different calcium phosphate nanoparticles, namely bovine bone bioapatite calcined at 500, 600, and 700°C, Mg‐doped brushite, fluorinated calcium phosphate, Ca‐deficient hydroxyapatite, hydroxyapatite, and tricalcium phosphate, were characterized employing physico‐chemical methods. Their cytoco...

Full description

Saved in:
Bibliographic Details
Published in:Journal of the American Ceramic Society Vol. 102; no. 5; pp. 2882 - 2904
Main Authors: Šupová, Monika, Suchý, Tomáš, Sucharda, Zbyněk, Filová, Elena, Kinderen, Job N. L. M., Steinerová, Marie, Bačáková, Lucie, Martynková, Gražyna Simha
Format: Journal Article
Language:English
Published: Columbus Wiley Subscription Services, Inc 01-05-2019
Subjects:
Apatite
Biocompatibility
Biomedical materials
Calcium ions
Calcium phosphates
Chemical composition
Correlation analysis
Elution
Fluorination
Hydroxyapatite
Ion concentration
Magnesium
Nanoparticles
Organic chemistry
Parameter identification
Surgical implants
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Eight different calcium phosphate nanoparticles, namely bovine bone bioapatite calcined at 500, 600, and 700°C, Mg‐doped brushite, fluorinated calcium phosphate, Ca‐deficient hydroxyapatite, hydroxyapatite, and tricalcium phosphate, were characterized employing physico‐chemical methods. Their cytocompatibility was evaluated under human osteoblast‐like cell line MG‐63 culture conditions in elution media and via the direct interaction of cells with calcium phosphate nanoparticles. The main objective was to determine the correlation of the cell indices with the differently determined physical and chemical parameters of the calcium phosphates. Chemical composition, which contributes toward pH changes, and the calcium ion concentration in the medium appear to make up particularly significant factors; moreover, it was proved that the number of material types represents a further important aspect. In the case of a large number of material types, almost no correlation was determined between the analyzed parameters; however, in the case of a small number of apatite types, several positive correlations were found. It can be concluded that it is not possible to identify any monitored parameters that had a major impact on cell behavior or, at least, such an effect which can be generalized. It appears more likely that cell behavior is affected by the interplay of various parameters.
ISSN:0002-7820
1551-2916
DOI:10.1111/jace.16110