Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, However, Their Intracellular Incorporation by Endocytosis Was Not Involved in This Effect on RAW264 Cells

Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, However, Their Intracellular Incorporation by Endocytosis Was Not Involved in This Effect on RAW264 Cells

The aim of this study was to evaluate the involvement of nanoparticles prepared from Allium cepa L. as anti-inflammatory agents. In the present study, we identified nanoparticles from Allium cepa L. using the ultracentrifugation exosome purification method. The nanoparticles were referred to as 17,0...

Full description

Saved in:
Bibliographic Details
Published in:Molecules (Basel, Switzerland) Vol. 26; no. 9; p. 2763
Main Authors: Yamasaki, Masao, Yamasaki, Yumi, Furusho, Rina, Kimura, Hayaka, Kamei, Ichiro, Sonoda, Hiroko, Ikeda, Masahiro, Oshima, Tatsuya, Ogawa, Kenjiro, Nishiyama, Kazuo
Format: Journal Article
Language:English
Published: Basel MDPI AG 07-05-2021
MDPI
Subjects:
Allium cepa
Allium cepa L
Anti-inflammatory agents
Caveolae
Cytotoxicity
Endocytosis
Flavonoids
Food
Inflammation
Lipids
lipopolysaccharide
Lipopolysaccharides
Nanoparticles
nanovesicles
Nitric oxide
Onions
Physiology
Precipitates
Proteins
Ultracentrifugation
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this study was to evaluate the involvement of nanoparticles prepared from Allium cepa L. as anti-inflammatory agents. In the present study, we identified nanoparticles from Allium cepa L. using the ultracentrifugation exosome purification method. The nanoparticles were referred to as 17,000× g and 200,000× g precipitates, and they contained quercetins, proteins, lipids, and small-sized RNA. The nanoparticles inhibited nitric oxide production from lipopolysaccharide (LPS)-stimulated RAW264 cells without cytotoxic properties. Cellular incorporation was confirmed by laser microscopic observation after PKH26 staining. The inhibition of caveolae-dependent endocytosis and macropinocytosis significantly prevented the incorporation of the nanoparticles but had no effect on the inhibition of nitric oxide in RAW264 cells. Collectively, the identified nanoparticles were capable of inhibiting the LPS response via extracellular mechanisms. Taken together, the way of consuming Allium cepa L. without collapsing the nanoparticles is expected to provide an efficient anti-inflammatory effect.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26092763