NELL-1 promotes cell adhesion and differentiation via integrinβ1

NELL-1 promotes cell adhesion and differentiation via integrinβ1

NELL‐1 (Nel‐like molecule‐1) is a secreted osteogenic growth factor first identified in human craniosynostosis (CS) patients. NELL‐1 protein has been observed to promote bone and cartilage differentiation and to suppress adipogenesis in both in vitro and in vivo models. Despite these findings, the c...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cellular biochemistry Vol. 113; no. 12; pp. 3620 - 3628
Main Authors: Shen, Jia, James, Aaron W., Chung, Jonguk, Lee, Kiho, Zhang, James B., Ho, Stephanie, Lee, Kevin S., Kim, Toyong M., Niimi, Tomoaki, Kuroda, Shun'ichi, Ting, Kang, Soo, Chia
Format: Journal Article
Language:English
Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-12-2012
Subjects:
3T3 Cells
Amino Acid Sequence
Animals
Blotting, Western
CELL ADHERENCE
Cell Adhesion
Cell Differentiation
Cell Proliferation
FAK
Focal Adhesions
Humans
Immunohistochemistry
Integrin beta1 - genetics
Integrin beta1 - metabolism
INTEGRINβ1
Lactic Acid - metabolism
Mice
Molecular Sequence Data
NELL-1
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Osteogenesis
OSTEOGENIC DIFFERENTIATION
Polyglycolic Acid - metabolism
Protein Binding
Protein Interaction Mapping
RNA, Small Interfering
Sequence Alignment
ST2
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:NELL‐1 (Nel‐like molecule‐1) is a secreted osteogenic growth factor first identified in human craniosynostosis (CS) patients. NELL‐1 protein has been observed to promote bone and cartilage differentiation and to suppress adipogenesis in both in vitro and in vivo models. Despite these findings, the cell surface receptors of NELL‐1 have remained unknown. In this study, we observed for the first time that NELL‐1 promotes cell adherence in multiple cell lines, including ST2, C3H10T1/2, M2‐10B4, ATDC5, and MC3T3 cells. Additionally, we found that NELL‐1 binds to extracellular Integrinβ1 and induces cell focal adhesion. By utilizing siRNA methods, we determined that NELL‐1 cell surface binding and enhanced cell attachment were dependent on Integrinβ1 expression. Finally, we observed that pre‐coating of culture dishes or PLGA (polylactic‐co‐glycolic acid) scaffold with NELL‐1 resulted in a significant increase in both cell attachment and osteogenic differentiation. Our results identify for the first time a cell surface target of NELL‐1, Integrinβ1, and elucidate new functions of NELL‐1 in promoting cell adherence and osteogenic differentiation. J. Cell. Biochem. 113: 3620–3628, 2012. © 2012 Wiley Periodicals, Inc.
Bibliography:T32 Training Fellowships - No. 5T32DE007296-14
istex:E1885892210D1BA6700636FA8F9BBFB69ED0DFE5
ark:/67375/WNG-6T0RMJXF-H
UC Discovery Grant - No. 07-10677
NIH/NIDCR - No. R21 DE0177711; No. RO1 DE01607
ArticleID:JCB24253
Disclosures: Drs. K.T. and C.S. are inventors of Nell-1 related patents. Drs. K.T. and C.S. are founders of Bone Biologics, Inc., which sublicenses Nell-1 patents from the UC Regents.
CIRM Early Translation II Research Award - No. TR2-01821
Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA Innovation Award
Disclosures: Drs. K.T. and C.S. are inventors of Nell‐1 related patents. Drs. K.T. and C.S. are founders of Bone Biologics, Inc., which sublicenses Nell‐1 patents from the UC Regents.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.24253