Role of the Inducible Adhesin CpAls7 in Binding of Candida parapsilosis to the Extracellular Matrix under Fluid Shear
The yeast is an increasingly common cause of systemic fungal infections among immunocompromised individuals, including premature infants. Adhesion to host surfaces is an important step in pathogenesis, but this process has not been extensively studied in this organism. A microfluidics assay was deve...
Saved in:
| Published in: | Infection and immunity Vol. 86; no. 4 |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
American Society for Microbiology
01-04-2018
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The yeast
is an increasingly common cause of systemic fungal infections among immunocompromised individuals, including premature infants. Adhesion to host surfaces is an important step in pathogenesis, but this process has not been extensively studied in this organism. A microfluidics assay was developed to test the ability of
to adhere to immobilized host extracellular matrix proteins under physiological fluid shear conditions. Growth in mammalian tissue culture medium at 37°C for 3 to 6 h led to the induction of an adhesive phenotype at shear forces of 1 to 5 dynes/cm
in some isolates of
Glutamic acid, proline, and calcium appeared to be the minimally necessary requirements for increased adhesion in these assays. To determine whether genes homologous to the
gene family of
were important for the adhesive phenotype, the expression levels of 5 homologous
genes were quantified by using quantitative PCR (qPCR) under conditions leading to increased adhesion.
(
) and
showed increased expression levels compared to those in control yeast. The extent of adhesion was variable among different isolates, and linear regression identified the expression of
but not
as having a strong positive correlation with adhesion. A homozygous
deletion strain was deficient in adhesion, whereas the expression of
in
resulted in increased adhesion. Together, these data provide strong evidence that CpAls7 aids in the adherence of
to the extracellular matrix under shear forces and support its previously reported role in virulence. |
|---|---|
| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 S.K.S. and J.M.B. contributed equally. Citation Neale MN, Glass KA, Longley SJ, Kim DJ, Laforce-Nesbitt SS, Wortzel JD, Shaw SK, Bliss JM. 2018. Role of the inducible adhesin CpAls7 in binding of Candida parapsilosis to the extracellular matrix under fluid shear. Infect Immun 86:e00892-17. https://doi.org/10.1128/IAI.00892-17. Present address: Kyle A. Glass, Boston University, Boston, Massachusetts, USA; Jeremy D. Wortzel, University of Pennsylvania, Philadelphia, Pennsylvania, USA. |
| ISSN: | 0019-9567 1098-5522 |
| DOI: | 10.1128/IAI.00892-17 |