Detection of human κ-opioid antibody using microresonators with integrated optical readout

Label-free detection of the interaction between hexahistidine-tagged human κ-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. Shift in resonance frequency due to accret...

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Bibliographic Details
Published in:	Biosensors & bioelectronics Vol. 26; no. 1; pp. 195 - 201
Main Authors:	Timurdogan, Erman, Ozber, Natali, Nargul, Sezin, Yavuz, Serhat, Kilic, M. Salih, Kavakli, I. Halil, Urey, Hakan, Alaca, B. Erdem
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier B.V 15-09-2010 Elsevier
Subjects:	Actuators Antibodies Antibodies - analysis Antibodies - immunology Bioelectricity Biological and medical sciences Biosensing Techniques - instrumentation Biosensors Biotechnology Devices Diffraction grating interferometry Electromagnetic actuation Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology Human Immunoassay - instrumentation Integrated readout Liquids Methods. Procedures. Technologies Micro-Electrical-Mechanical Systems - instrumentation Microcantilever immunosensor Miniaturization Optical Devices Protein–antibody interaction Receptors Receptors, Opioid, kappa - analysis Receptors, Opioid, kappa - immunology Refractometry - instrumentation Resonator Various methods and equipments Resonator Microcantilever immunosensor Electromagnetic actuation Integrated readout Protein–antibody interaction Diffraction grating interferometry Human Antibody Interaction Immunosensor Protein Protein-antibody interaction Diffraction Biosensor Detection Interferometry
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Summary:	Label-free detection of the interaction between hexahistidine-tagged human κ-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. Shift in resonance frequency due to accretion of mass on the sensitive surface of microresonators is monitored via an integrated optical readout. A frequency resolution of 2 Hz is obtained. Together with a sensitivity of 7 ppm/(ng/ml) this leads to a minimum detectable antibody concentration of 5.7 ng/ml for a 50-kHz device. The measurement principle is shown to impart immunity to environmental noise, facilitate operation in liquid media and bring about the prospect for further miniaturization of actuator and readout leading to a portable biochemical sensor.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0956-5663 1873-4235
DOI:	10.1016/j.bios.2010.06.004