Search Results - "Kilburn, D.G."

The adsorption of a bacterial cellulase and its two isolated domains to crystalline cellulose by Gilkes, N.R. (University of British Columbia, Vancouver, British Columbia, Canada), Jervis, E, Henrissat, B, Tekant, B, Miller, R.C. Jr, Warren, R.A.J, Kilburn, D.G

“…CenA is a bacterial cellulase (beta-1,4-glucanase) comprised of a globular catalytic domain joined to an extended cellulose-binding domain (CBD) by a short…”
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Inexpensive and Generic Affinity Purification of Recombinant Proteins Using a Family 2a CBM Fusion Tag by Rodriguez, Beatriz, Kavoosi, Mojgan, Koska, Jürgen, Creagh, A. Louise, Kilburn, Douglas G., Haynes, Charles A.

“…The selective binding of the family 2a carbohydrate binding module (CBM2a) of xylanase 10A of the soil bacterium Cellulomonas fimi to a variety of cellulosic…”
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Stereoselective hydrolysis catalyzed by related beta-1,4-glucanases and beta-1,4-xylanases by J Gebler, N R Gilkes, M Claeyssens, D B Wilson, P Béguin, W W Wakarchuk, D G Kilburn, R C Miller, Jr, R A Warren, S G Withers

“…Over 80 beta-1,4-glucanases and beta-1,4-xylanases can be classified into one of eight families on the basis of amino acid sequence similarities in their…”
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Glutamic acid 274 is the nucleophile in the active site of a "retaining" exoglucanase from Cellulomonas fimi by TULL, D, WITHERS, S. G, GILKES, N. R, KILBURN, D. G, ANTONY, R, WARREN, R. A. J, AEBERSOLD, R

“…In addition to its known substrate activity with p-nitrophenyl beta-cellobioside, the exoglucanase from Cellulomonas fimi has been shown to utilize substituted…”
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Deletion of the linker connecting the catalytic and cellulose-binding domains of endoglucanase A (CenA) of Cellulomonas fimi alters its conformation and catalytic activity by H Shen, M Schmuck, I Pilz, N R Gilkes, D G Kilburn, R C Miller, Jr, R A Warren

“…The Pro-Thr box is a linker of 23 amino acids ((PT)4T(PT)7) connecting the catalytic domain and the cellulose-binding domain (CBD) of endoglucanase A (CenA)…”
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Glycosylation of bacterial cellulases prevents proteolytic cleavage between functional domains by Langsford, M.L., Gilkes, N.R., Singh, B., Moser, B., Miller, R.C., Warren, R.A.J., Kilburn, D.G.

“…Glycosylated cellulases from Cellulomonas fimi were compared with their non-glycosylated counterparts synthesized in Escherichia coli from recombinant DNA…”
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Degradation of cellulases in cultures of Cellulomonas fimi by Sandercock, L.E, Meinke, A, Gilkes, N.R, Kilburn, D.G, Warren, R.A.J

“…Endoglucanases CenA, CenB and CenD, cellobiohydrolases CbhA and CbhB, and the mixed function xylanase-exoglucanase Cex are degraded proteolytically in the…”
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Structure of the gene encoding the exoglucanase of Cellulomonas fimi by O'Neill, G, Goh, S H, Warren, R A, Kilburn, D G, Miller, Jr, R C

“…In Cellulomonas fimi the cex gene encodes an exoglucanase (Exg) involved in the degradation of cellulose. The gene now has been sequenced as part of a 2.58-kb…”
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Stereochemical course of hydrolysis catalysed by Cellulomonas fimi CenE, a member of a new family of beta-1,4-glucanases by Shen, H, Tomme, P, Meinke, A, Gilkes, N R, Kilburn, D G, Warren, R A, Miller, Jr, R C

“…The gene for a previously identified, extracellular, 120 kDa cellulose-binding protein (Cbp120) was isolated from a Cellulomonas fimi genomic library and…”
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Rational design and PCR-based synthesis of an artificial Schizophyllum commune xylanase gene by Graham, R.W, Atkinson, T, Kilburn, D.G, Miller, R.C. Jr, Warren, R.A.J

“…A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Three long oligonucleotides were synthesized…”
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The expression of Cellulomonas fimi cellulase genes in Brevibacterium lactofermentum by Paradis, F W, Warren, R A, Kilburn, D G, Miller, Jr, R C

“…The exoglucanase gene (cex) and the endoglucanase A gene (cenA) from Cellulomonas fimi were subcloned into the Escherichia coli/Brevibacterium lactofermentum…”
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A bifunctional exoglucanase-endoglucanase fusion protein by Warren, R A, Gerhard, B, Gilkes, N R, Owolabi, J B, Kilburn, D G, Miller, Jr, R C

“…A fusion was constructed between the cex gene of Cellulomonas fimi, which encodes an exoglucanase, and the cenA gene of the same organism, which encodes an…”
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Mannanase Man26A from Cellulomonas fimi has a mannan-binding module by Stoll, D, Boraston, A, Stalbrand, H, McLean, B.W, Kilburn, D.G, Warren, R.A.J

“…A modular mannanase (Man26A) from the bacterium Cellulomonas fimi contains a mannan-binding module (Man26Abm) that binds to soluble but not to insoluble…”
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Comparison of a fungal (family I) and bacterial (family II) cellulose-binding domain by Tomme, P. (University of British Columbia, Vancouver, BC, Canada.), Driver, D.P, Amandoron, E.A, Miller, R.C. Jr, Antony, R, Warren, J, Kilburn, D.G

“…A family II cellulose-binding domain (CBD) of an exoglucanase/xylanase (Cex) from the bacterium Cellulomonas fimi was replaced with the family I CBD of…”
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Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor-cellulose-binding domain fusion protein by Doheny, J.G, Jervis, E.J, Guarna, M.M, Humphries, R.K, Warren, R.A.J, Kilburn, D.G

“…A chimaera of stem cell factor (SCF) and a cellulose-binding domain from the xylanase Cex (CBDCex) effectively immobilizes SCF on a cellulose surface. The…”
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Enhancement of the Endo-β-1,4-glucanase Activity of an Exocellobiohydrolase by Deletion of a Surface Loop (∗) by Meinke, Andreas, Damude, Howard G., Tomme, Peter, Kwan, Emily, Kilburn, Douglas G., Miller, Robert C., Warren, R.Antony J., Gilkes, Neil R.

“…In the commonly accepted mechanism for enzymatic hydrolysis of cellulose, endo-β-1,4-glucanases randomly cleave glucosidic bonds within glucan polymers,…”
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Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi by Shen, H, Gilkes, N.R, Kilburn, D.G, Miller, R.C. Jr, Warren, R.A.J

“…The gene cbhB from the cellulolytic bacterium Cellulomonas fimi encodes a polypeptide of 1090 amino acids. Cellobiohydrolase B (CbhB) is 1037 amino acids long,…”
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Self-activating factor X derivative fused to the C-terminus of a cellulose-binding module: Production and properties by Kwan, Emily, Guarna, M. Marta, Boraston, Alisdair B., Gilkes, Neil R., Haynes, Charles A., Kilburn, Douglas G., Warren, R. Antony J.

“…In this work, a new derivative of FX was engineered. It comprises a cellulose‐binding module (CBM) fused to the N‐terminus of the truncated light chain (E2FX)…”
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Expression Analysis of a Modified Factor X in Stably Transformed Insect Cell Lines by Pfeifer, T.A., Guarna, M.M., Kwan, E.M., Lesnicki, G., Theilmann, D.A., Grigliatti, T.A., Kilburn, D.G.

“…A modified Factor X protein was combined with a cellulose-binding domain tag and expressed in insect cell lines. The protein, CBDFX, was expressed and secreted…”
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Analysis of Molecular Size Distributions of Cellulose Molecules during Hydrolysis of Cellulose by Recombinant Cellulomonas fimiβ-1,4-Glucanases by STALBRAND, H, MANSFIELD, S. D, SADDLER, J. N, KILBURN, D. G, WARREN, R. A. J, GILKES, N. R

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