Highly efficient transformation of the model zygnematophycean alga Closterium peracerosum‐strigosum‐littorale complex by square‐pulse electroporation

Highly efficient transformation of the model zygnematophycean alga Closterium peracerosum‐strigosum‐littorale complex by square‐pulse electroporation

Summary The zygnematophycean algae occupy an important phylogenetic position as the closest living relatives of land plants. Reverse genetics is quite useful for dissecting the functions of genes. However, this strategy requires genetic transformation, and there are only a few reports of successful...

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Bibliographic Details
Published in:The New phytologist Vol. 233; no. 1; pp. 569 - 578
Main Authors: Kawai, Junko, Kanazawa, Manaki, Suzuki, Rie, Kikuchi, Nanako, Hayakawa, Yasuhiko, Sekimoto, Hiroyuki
Format: Journal Article
Language:English
Published: England Wiley Subscription Services, Inc 01-01-2022
Subjects:
Algae
Cell walls
Closterium
Closterium peracerosum
CRISPR
Electroporation
evolution
Gene editing
Genes
Genetic transformation
Genetics
Genomes
Incubators
Labour
Particle bombardment
Phylogeny
Plants
transformation
Transformation, Genetic
Transformations
Zygnematophyceae
Closterium
electroporation
evolution
transformation
Zygnematophyceae
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Summary:Summary The zygnematophycean algae occupy an important phylogenetic position as the closest living relatives of land plants. Reverse genetics is quite useful for dissecting the functions of genes. However, this strategy requires genetic transformation, and there are only a few reports of successful transformation in zygnematophycean algae. Here, we established a simple and highly efficient transformation technique for the unicellular zygnematophycean alga Closterium peracerosum‐strigosum‐littorale complex using a square electric pulse‐generating electroporator without the need for cell wall removal. Using this method, the transformation efficiency increased > 100‐fold compared with our previous study using particle bombardment. We also succeeded in performing CRISPR/Cas9‐based gene knockout using this new method. Our method requires only small amounts of labor, time and incubator space. Moreover, our technique could also be utilized to transform other charophycean algae with available genome information by optimizing the electric pulse conditions.
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ISSN:0028-646X
1469-8137
DOI:10.1111/nph.17763