Simultaneous harvesting and cell disruption of microalgae using ozone bubbles: optimization and characterization study for biodiesel production

Simultaneous harvesting and cell disruption of microalgae using ozone bubbles: optimization and characterization study for biodiesel production

In the present study, ozone was introduced as an alternative approach to harvest and disrupt microalgae cells ( Chlorella vulgaris ) simultaneously for biodiesel production. At the optimum ozonation conditions (6.14 g·h −1 ozone concentration, 30 min ozonation time, 1 L·min −1 of ozone flowrate at m...

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Bibliographic Details
Published in:Frontiers of chemical science and engineering Vol. 15; no. 5; pp. 1257 - 1268
Main Authors: Kadir, Wan N. A., Lam, Man K., Uemura, Yoshimitsu, Lim, Jun W., Kiew, Peck L., Lim, Steven, Rosli, Siti S., Wong, Chung Y., Show, Pau L., Lee, Keat T.
Format: Journal Article
Language:English
Published: Beijing Higher Education Press 01-10-2021
Springer Nature B.V
Subjects:
Algae
Biodiesel fuels
Chemistry
Chemistry and Materials Science
Industrial Chemistry/Chemical Engineering
Lipids
Nanotechnology
Optimization
Ozone
Research Article
Surface charge
microalgae
ozonation
pre-treatment
lipid
biodiesel
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Summary:In the present study, ozone was introduced as an alternative approach to harvest and disrupt microalgae cells ( Chlorella vulgaris ) simultaneously for biodiesel production. At the optimum ozonation conditions (6.14 g·h −1 ozone concentration, 30 min ozonation time, 1 L·min −1 of ozone flowrate at medium pH of 10 and temperature of 30 °C), the sedimentation efficiency of microalgae cells increased significantly from 12.56% to 68.62%. It was observed that the microalgae cells aggregated to form flocs after pretreated with ozone due to the increment of surface charge from −20 to −6.59 mV. Besides, ozone had successfully disrupted the microalgae cells and resulted in efficient lipid extraction, which was 1.9 times higher than the control sample. The extracted microalgae lipid was mainly consisted of methyl palmitate (C16:0), methyl oleate (C18:1) and methyl linolenate (C18:3), making it suitable for biodiesel production. Finally, utilization of recycled culture media after ozonation pre-treatment showed robust growth of microalgae, in which the biomass yield was maintained in the range of 0.796 to 0.879 g ·h −1 for 5 cycles of cultivation.
ISSN:2095-0179
2095-0187
DOI:10.1007/s11705-020-2015-9