Prokineticin receptor identified by phage display is an entry receptor for Trypanosoma cruzi into mammalian cells

Prokineticin receptor identified by phage display is an entry receptor for Trypanosoma cruzi into mammalian cells

Trypanosoma cruzi trypomastigotes invade a great variety of mammalian cells, with several molecules being implicated in this complex event. Herein, the sequence GGIALAG present in prokineticin-2 receptor (PKR2), selected by phage display technology, is described as a new T. cruzi receptor for the Tc...

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Bibliographic Details
Published in:Parasitology research (1987) Vol. 114; no. 1; pp. 155 - 165
Main Authors: Khusal, K. G, Tonelli, R. R, Mattos, E. C, Soares, C. O, Di Genova, B. M, Juliano, M. A, Urias, U, Colli, W, Alves, M. J. M
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer-Verlag 2015
Springer Berlin Heidelberg
Springer
Subjects:
Animals
antibodies
Bacteriophages
Binding Sites
Biomedical and Life Sciences
Biomedicine
blood cells
Cell interaction
cell invasion
Cell Line
central nervous system
Conserved Sequence
glycoproteins
Glycoproteins - genetics
Health aspects
Host-parasite relationships
hosts
Humans
Identification and classification
Immunology
Medical Microbiology
Microbiology
Original Paper
parasites
receptors
Receptors, G-Protein-Coupled - genetics
Receptors, G-Protein-Coupled - metabolism
Receptors, Peptide - genetics
Receptors, Peptide - metabolism
RNA interference
Trypanosoma cruzi
Trypanosoma cruzi - physiology
trypomastigotes
Phage display
invasion
iRNA
Prokineticin-2 receptor
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Summary:Trypanosoma cruzi trypomastigotes invade a great variety of mammalian cells, with several molecules being implicated in this complex event. Herein, the sequence GGIALAG present in prokineticin-2 receptor (PKR2), selected by phage display technology, is described as a new T. cruzi receptor for the Tc85 group of glycoproteins belonging to the gp85/TS superfamily and involved in cellular invasion of mammalian hosts. This finding is confirmed by the inhibitory activity of MCF10-A (human mammary) cell invasion by T. cruzi either by anti-PKR2 antibodies (77 %) or GGIALAG-synthetic peptide (42 %). Furthermore, interference RNA (iRNA) inhibition of PKR2 expression in MCF10-A cells reduces T. cruzi invasion by 50 %. The binding site of Tc85 to PKR2 was localized at the C-terminal end of the molecule, upstream of the conserved FLY sequence, previously implicated in parasite cell invasion. PKR2, a receptor formed by seven membrane-spanning α-helical segments, is mainly present in the central nervous system, peripheral organs, and mature blood cells. Due to its wide distribution, PKR2 could be a suitable receptor for T. cruzi natural infection, contributing to the parasite dissemination throughout the mammalian organism. These findings augment the number and diversity of possible in vivo receptors for T. cruzi and reassure the multiplicity of Tc85 binding sites to mammalian hosts.
Bibliography:http://dx.doi.org/10.1007/s00436-014-4172-6
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ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-014-4172-6