Enhanced Hyaluronic Acid Production from Priestia flexa N7 Isolates

Background: Hyaluronic acid (HA) is a gel-like substance made up of glucuronic acid and N-acetylglucosamine units, capable of absorbing and retaining water, present in hydrated gel form across human and animal tissues. It aids in joint lubrication and moisture retention and acts as a cushion for sho...

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Bibliographic Details
Published in:	Biomedical and biotechnology research journal Vol. 8; no. 1; pp. 19 - 26
Main Authors:	Vu, Dinh Giap, van Do, Thi Cam, Thi, Linh Mai Dau, Dang, Danh Phong, Khuat, Bich Ngoc Thi, Thi, Oanh Kim Hoang, Do, Thu Minh, Ha, Thi Dung, Mac, The Vinh, Nguyen, Phuong Dai Nguyen
Format:	Journal Article
Language:	English
Published:	Wolters Kluwer Medknow Publications 01-01-2024
Subjects:	hyaluronic acid microorganisms molecular biological optimal conditions priestia flexa n7
Online Access:	Get full text
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Summary:	Background: Hyaluronic acid (HA) is a gel-like substance made up of glucuronic acid and N-acetylglucosamine units, capable of absorbing and retaining water, present in hydrated gel form across human and animal tissues. It aids in joint lubrication and moisture retention and acts as a cushion for shock absorption. HA has unique biological properties, promoting fibroblast cell growth, aiding wound healing, and exhibiting low solubility and viscosity, making it an organic ingredient in tissue culture techniques. It is utilized in eye drops and skin ointments and plays a vital role in the extracellular matrix, rendering it invaluable in medical and cosmetic applications, such as treating osteoarthritis and enhancing skin wound recovery. Methods: The methods employed in this study involve isolating microorganisms, screening bacterial strains capable of synthesizing HA, identifying bacteria using molecular biological methods, and researching optimal conditions to select bacterial strains that produce the highest HA concentrations. Results: In this study, strain Priestia flexa N7 was studied for suitable conditions for HA biosynthesis. Bacterial strains were fermented for 48 h on medium containing the following ingredients: glucose (60 g/L); yeast extract (5.0 g/L); peptone (20 g/L); K2HPO4 (2.0 g/L); Na2HPO4 (1.0 g/L); NaCl (2.0 g/L); FeSO4 (1.0 g/l); sodium glutamate (9.0 g/L); and MgSO4.7H2O (2.0 g/L) and pH 8.0 at 37°C under the condition of continuous shaking at 150 rpm. The maximum HA production achieved was 1105 mg/L. Conclusions: The mentioned bacterial strain exhibits significant potential for HA synthesis and is extensively employed in producing items across the health care, medical, food, and cosmetic industries. These findings revealed the most effective HA acid manufacturing strategy for achieving maximum output.
ISSN:	2588-9834 2588-9842
DOI:	10.4103/bbrj.bbrj_301_23