Generation and characterization of polyclonal antibodies specific to N-terminal extension of p85 isoform of ribosomal protein S6 kinase 1 (p85 S6K1)

Generation and characterization of polyclonal antibodies specific to N-terminal extension of p85 isoform of ribosomal protein S6 kinase 1 (p85 S6K1)

Aim. Generation of polyclonal antibodies specific to the ribosomal protein S6 kinase isoform – p85S6K1 and directed to the N-terminal (1–23 aa) extension of p85S6K1. Methods. Animal immunization with synthetic (1–23 aa) peptide, ELISA, Western blot, Immunoprecipitation, immunofluorescent analysis. R...

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Bibliographic Details
Published in:Biopolimery i kletka Vol. 31; no. 4; pp. 294 - 300
Main Authors: Savinska, L. O., Klipa, O. M., Khoruzenko, A. I., Shkarina, K. S., Garifulin, O. M., Filonenko, V. V.
Format: Journal Article
Language:English
Published: Kiev Natsional'na Akademiya Nauk Ukrainy - National Academy of Sciences of Ukraine 2015
Subjects:
Enzyme-linked immunosorbent assay
Immunization
Immunofluorescence
Immunoprecipitation
Isoforms
Localization
Polyclonal antibodies
Proteins
Ribosomal protein S6
Ribosomal protein S6 kinase
Signal transduction
Transduction
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Summary:Aim. Generation of polyclonal antibodies specific to the ribosomal protein S6 kinase isoform – p85S6K1 and directed to the N-terminal (1–23 aa) extension of p85S6K1. Methods. Animal immunization with synthetic (1–23 aa) peptide, ELISA, Western blot, Immunoprecipitation, immunofluorescent analysis. Results. Polyclonal antibodies have been generated, which specifically recognize only p85 but not p70 isoform of S6K1 in western blot, immunoprecipitation and immunofluorescence analysis. Conclusions. The obtained antibodies can be recommended for studies on the p85S6K1 and other S6K1 isoforms possessing the N-terminal extension – the identification of binding protein partners, analysis of subcellular localization under different physiological conditions, elucidation of the signal transduction pathways involving different S6K1 isoforms.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.0008EE