Upregulation of Gingival Tissue miR-200b in Obese Periodontitis Subjects

Increased local immune and inflammatory responses in obese individuals with periodontitis may explain the aggressive clinical presentation and altered treatment response when compared to that of normal weight subjects. Our goal was to identify any differences in microRNA (miRNA) expression profiles...

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Bibliographic Details
Published in:	Journal of dental research Vol. 94; no. 3_suppl; pp. 59S - 69S
Main Authors:	Kalea, A.Z., Hoteit, R., Suvan, J., Lovering, R.C., Palmen, J., Cooper, J.A., Khodiyar, V.K., Harrington, Z., Humphries, S.E., D’Aiuto, F.
Format:	Journal Article
Language:	English
Published:	Los Angeles, CA SAGE Publications 01-03-2015 SAGE PUBLICATIONS, INC
Subjects:	Adult Angiogenesis Bioinformatics Biomedical research Biopsy Body Weight Cell differentiation Cell Differentiation - genetics Cell Movement - genetics Clinical Investigations Data analysis DNA-Binding Proteins - genetics Female Funding GATA2 Transcription Factor - analysis Gene expression Gene Expression Profiling Genomics Gingiva Gingiva - metabolism Gum disease Homeodomain Proteins - analysis Humans Inflammation Male Medical research MicroRNAs MicroRNAs - analysis miRNA Neovascularization, Physiologic - genetics Obesity Obesity - genetics Patients Periodontitis Periodontitis - genetics Polymerase chain reaction Repressor Proteins - analysis Research centers Reverse Transcriptase Polymerase Chain Reaction - methods Reverse transcription Studies Teeth Therapeutic applications Transcription Factors - analysis University colleges Up-Regulation Vascular Endothelial Growth Factor Receptor-2 - analysis Wound healing Zinc Finger E-box Binding Homeobox 2 Zinc Finger E-box-Binding Homeobox 1 Zinc Fingers - genetics United Kingdom > UK United States > US epithelial wound healing angiogenesis obesity periodontal disease microRNA-200b
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Summary:	Increased local immune and inflammatory responses in obese individuals with periodontitis may explain the aggressive clinical presentation and altered treatment response when compared to that of normal weight subjects. Our goal was to identify any differences in microRNA (miRNA) expression profiles of gingival tissue in periodontitis when obesity is present, which may suggest novel molecular pathways that this miRNA network may affect. Total RNA was extracted from gingival tissue biopsies collected from normal weight and obese individuals with periodontitis; miRNA expression profiling was performed with Affymetrix GeneChip miRNA 3.0 arrays; and results were validated with quantitative reverse transcription polymerase chain reaction (qRT-PCR). In silico identification of previously confirmed miRNA gene targets was conducted through miRTarBase and miRWalk databases, and pathway enrichment analysis identified enriched miRNA gene sets. Expression of selected genes in the same biopsy samples was tested with qRT-PCR. The gingival tissue miRNA profile of obese patients, compared to that of normal weight patients, showed 13 upregulated and 22 downregulated miRNAs, among which miR-200b was validated by qRT-PCR to be significantly increased in obesity. Functional analysis of 51 experimentally validated miR-200b gene targets identified enrichment of genes involved in cell motility, differentiation, DNA binding, response to stimulus, and vasculature development pathways not previously identified in the obesity-specific disease profile. Furthermore, the expression of the miR-200b gene targets ZEB1/2, GATA2, and KDR was confirmed by qRT-PCR as being lower in obese patients with periodontitis versus normal weight patients, suggesting a role of miR-200b in regulation of a set of gene targets and biological pathways relevant to wound healing and angiogenesis. Functional studies to explore the role of miR-200b in the above processes may offer new insights on putative therapeutic targets for this group of patients.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-0345 1544-0591
DOI:	10.1177/0022034514568197