Incidence of Mycobacterium Avium Subspecies paratuberculosis in Mehsani and Surti Goats of Indian Origin using Multiple Diagnostic Tests

This paper aims to estimate the incidence of Mycobacterium avium subspecies paratuberculosis (MAP) in two important breeds of goats, namely Mehsani and Surti, from South Gujarat region of India by applying conventional, molecular and serological methods. Percent positivity by faecal smear examinatio...

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Bibliographic Details
Published in:	Journal of biological sciences (Faisalabad, Pakistan) Vol. 14; no. 2; pp. 124 - 133
Main Authors:	Barad, D.B., Chandel, B.S., Dadawala, A.I., Chauhan, H.C., Kher, H.S., Shroff, S., Bhagat, A.G., Singh, S.V., Singh, P.K., Singh, A.V., Sohal, J.S., Gupta, S., Chaubey, K.K., Chakrabort, S., Tiwari, R., Deb, R., Dhama, K.
Format:	Journal Article
Language:	English
Published:	2014
Subjects:	Mycobacterium avium
Online Access:	Get full text
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Summary:	This paper aims to estimate the incidence of Mycobacterium avium subspecies paratuberculosis (MAP) in two important breeds of goats, namely Mehsani and Surti, from South Gujarat region of India by applying conventional, molecular and serological methods. Percent positivity by faecal smear examination, delayed type hypersensitivity (DTH), agar gel immunodiffusion, IS900 polymerase chain reaction and indigenous enzyme linked immunosorbent assay (ELISA) kit was 9.2%, 21.9%, 10.9%, 12.5% and 43.3%, respectively. Similar to these findings, sensitivity of Johnin test in goats ranged between 18%-30% with least specificity in both preclinical and advanced stage of disease. Rectal pinch smear examination was carried out in 27 DTH positive goats and all smears were negative for the presence of acid fast bacilli. Screening tests (Indigenous ELISA and DTH) showed very high incidence of MAP infection in the goat population. The utility of multiple diagnostic tests is suggested for confirmatory detection and epidemiological diseases investigations of MAP in animals.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1727-3048 1812-5719
DOI:	10.3923/jbs.2014.124.133