HPLC analysis and cytotoxic activity of Vernonia cinerea

HPLC analysis and cytotoxic activity of Vernonia cinerea

The extracts of five Cambodian medicinal plants (Aganosma marginata, Dracaena cambodiana, Harrisonia perforata, Hymenodictyon excelsum and Vernonia cinerea) were evaluated in vitro for their cytotoxic activity against HT29 colon adenocarcinoma cells and HepG2 hepatoma cells, using the MTT assay. Amo...

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Bibliographic Details
Published in:Natural product communications Vol. 7; no. 10; p. 1259
Main Authors: Khay, Mom, Toeng, Phirom, Mahiou-Leddet, Valérie, Mabrouki, Fathi, Sothea, Kim, Ollivier, Evelyne, Elias, Riad, Bun, Sok-Siya
Format: Journal Article
Language:English
Published: United States 01-10-2012
Subjects:
Antineoplastic Agents, Phytogenic - analysis
Antineoplastic Agents, Phytogenic - pharmacology
Cambodia
Chromatography, High Pressure Liquid
Drug Screening Assays, Antitumor
HT29 Cells
Humans
Indicators and Reagents
Magnetic Resonance Spectroscopy
Methanol
Methylene Chloride
Sesquiterpenes - chemistry
Sesquiterpenes - isolation & purification
Sesquiterpenes - pharmacology
Solvents
Tetrazolium Salts
Thiazoles
Vernonia - chemistry
Water
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Summary:The extracts of five Cambodian medicinal plants (Aganosma marginata, Dracaena cambodiana, Harrisonia perforata, Hymenodictyon excelsum and Vernonia cinerea) were evaluated in vitro for their cytotoxic activity against HT29 colon adenocarcinoma cells and HepG2 hepatoma cells, using the MTT assay. Among these five plants, Vernonia cinerea displayed potent cytotoxicity. One main sesquiterpene lactone, 8alpha-tigloyloxy-hirsutinolide-13-O-acetate was isolated from the whole plant of V. cinerea. This compound was active against both cancer cell lines (IC50 = 3.50 microM for HT29 and IC50 = 4.27 microM for HepG2). To quantify this compound in the plant, an analytical high-performance liquid chromatography (HPLC) method was developed and validated.
ISSN:1934-578X
DOI:10.1177/1934578X1200701001